##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AseI AsnI AspEI AspI AviII
BamHI BfrI BsaBI BsiWI BspDI
BspHI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PflFI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 276 GGTACC G^GTACC
AccIII 5062 TCCGGA T^CCGGA
AgeI 3324 ACCGGT A^CCGGT
Alw44I 2829 GTGCAC G^TGCAC
AocI 3247 CCTGAGG CC^T[ACGT]AGG
ApaI 1156 GGGCCC GGGCC^C
ApaLI 2829 GTGCAC G^TGCAC
AscI 1355 GGCGCGCC GG^CGCGCC
Asp718I 276 GGTACC G^GTACC
AvrII 603 CCTAGG C^CTAGG
BbrPI 930 CACGTG CAC^GTG
BcgI 3663 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4917 AGATCT A^GATCT
BlnI 603 CCTAGG C^CTAGG
BsaAI 930 CACGTG [CT]AC^GT[GA]
BsaI 4083 GAGACC GGTCTC(1/5)
BseAI 5062 TCCGGA T^CCGGA
BsiEI 1196 CGACCG CG[GA][CT]^CG
BsmI 3587 GAATGC GAATGC(1/-1)
Bsp1407I 2632 TGTACA T^GTACA
BspEI 5062 TCCGGA T^CCGGA
BsrDI 608 GCAATG GCAATG(2/0)
BsrGI 2632 TGTACA T^GTACA
Bsu36I 3247 CCTGAGG CC^T[ACGT]AGG
DrdI 117 GACTCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3247 CCTGAGG CC^T[ACGT]AGG
EcoRI 4000 GAATTC G^AATTC
HincII 4015 GTCAAC GT[CT]^[GA]AC
HindII 4015 GTCAAC GT[CT]^[GA]AC
KpnI 276 GGTACC GGTAC^C
MroI 5062 TCCGGA T^CCGGA
PaeR7I 3671 CTCGAG C^TCGAG
PinAI 3324 ACCGGT A^CCGGT
PmaCI 930 CACGTG CAC^GTG
PmlI 930 CACGTG CAC^GTG
PpuMI 4322 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4397 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1595 AGTACT AGT^ACT
SspBI 2632 TGTACA T^GTACA
XhoI 3671 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 354 GTCTAC GT^[AC][GT]AC
2334 GTCTAC
AcsI 4000 GAATTC [GA]^AATT[CT]
5081 AAATTC
ApoI 4000 GAATTC [GA]^AATT[CT]
5081 AAATTC
Asp700I 2886 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4366 GAACGGCTTC
BalI 627 TGGCCA TGG^CCA
4186 TGGCCA
BbeI 1332 GGCGCC GGCGC^C
3518 GGCGCC
BbsI 477 GTCTTC GAAGAC(2/6)
4771 GTCTTC
BbvCI 2789 GCTGAGG CCTCAGC(-5/-2)
3280 GCTGAGG
BclI 711 TGATCA T^GATCA
5050 TGATCA
BpuAI 477 GTCTTC GAAGAC(2/6)
4771 GTCTTC
BsaWI 3324 ACCGGT [AT]^CCGG[AT]
5062 TCCGGA
BsmBI 1142 GAGACG CGTCTC(1/5)
3651 GAGACG
BspLU11I 362 ACATGT A^CATGT
2330 ACATGT
BssHII 1282 GCGCGC G^CGCGC
1356 GCGCGC
BssSI 1151 CACGAG CACGAG(-5/-1)
4554 CTCGTG
BstAPI 213 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3385 GCAGAAACTGC
BstEII 3447 GGTGACC G^GT[ACGT]ACC
4140 GGTCACC
BstPI 3447 GGTGACC G^GT[ACGT]ACC
4140 GGTCACC
DraIII 75 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2732 CACTGAGTG
EcoO65I 3447 GGTGACC G^GT[ACGT]ACC
4140 GGTCACC
FbaI 711 TGATCA T^GATCA
5050 TGATCA
KasI 1332 GGCGCC G^GCGCC
3518 GGCGCC
MluNI 627 TGGCCA TGG^CCA
4186 TGGCCA
MscI 627 TGGCCA TGG^CCA
4186 TGGCCA
NaeI 1363 GCCGGC GCC^GGC
2298 GCCGGC
NarI 1332 GGCGCC GG^CGCC
3518 GGCGCC
NgoMIV 1363 GCCGGC G^CCGGC
2298 GCCGGC
NheI 1879 GCTAGC G^CTAGC
2444 GCTAGC
SapI 3975 GCTCTTC GCTCTTC(1/4)
4048 GCTCTTC
SfoI 1332 GGCGCC GGC^GCC
3518 GGCGCC
StuI 822 AGGCCT AGG^CCT
2608 AGGCCT
XcmI 2758 CCAGCCTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3494 CCACGCGGCTCCTGG
XmnI 2886 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4366 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SgrAI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI