################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AflII AhdI Aor51HI AseI AsnI AspEI AspI AviII BamHI BfrI BsaBI BsiWI BspDI BspHI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HindIII HpaI KspI MamI MfeI MluI MunI NdeI NotI NruI NspV PacI PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 276 GGTACC G^GTACC AccIII 5062 TCCGGA T^CCGGA AgeI 3324 ACCGGT A^CCGGT Alw44I 2829 GTGCAC G^TGCAC AocI 3247 CCTGAGG CC^T[ACGT]AGG ApaI 1156 GGGCCC GGGCC^C ApaLI 2829 GTGCAC G^TGCAC AscI 1355 GGCGCGCC GG^CGCGCC Asp718I 276 GGTACC G^GTACC AvrII 603 CCTAGG C^CTAGG BbrPI 930 CACGTG CAC^GTG BcgI 3663 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 4917 AGATCT A^GATCT BlnI 603 CCTAGG C^CTAGG BsaAI 930 CACGTG [CT]AC^GT[GA] BsaI 4083 GAGACC GGTCTC(1/5) BseAI 5062 TCCGGA T^CCGGA BsiEI 1196 CGACCG CG[GA][CT]^CG BsmI 3587 GAATGC GAATGC(1/-1) Bsp1407I 2632 TGTACA T^GTACA BspEI 5062 TCCGGA T^CCGGA BsrDI 608 GCAATG GCAATG(2/0) BsrGI 2632 TGTACA T^GTACA Bsu36I 3247 CCTGAGG CC^T[ACGT]AGG DrdI 117 GACTCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3247 CCTGAGG CC^T[ACGT]AGG EcoRI 4000 GAATTC G^AATTC HincII 4015 GTCAAC GT[CT]^[GA]AC HindII 4015 GTCAAC GT[CT]^[GA]AC KpnI 276 GGTACC GGTAC^C MroI 5062 TCCGGA T^CCGGA PaeR7I 3671 CTCGAG C^TCGAG PinAI 3324 ACCGGT A^CCGGT PmaCI 930 CACGTG CAC^GTG PmlI 930 CACGTG CAC^GTG PpuMI 4322 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 4397 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1595 AGTACT AGT^ACT SspBI 2632 TGTACA T^GTACA XhoI 3671 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 354 GTCTAC GT^[AC][GT]AC 2334 GTCTAC AcsI 4000 GAATTC [GA]^AATT[CT] 5081 AAATTC ApoI 4000 GAATTC [GA]^AATT[CT] 5081 AAATTC Asp700I 2886 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4366 GAACGGCTTC BalI 627 TGGCCA TGG^CCA 4186 TGGCCA BbeI 1332 GGCGCC GGCGC^C 3518 GGCGCC BbsI 477 GTCTTC GAAGAC(2/6) 4771 GTCTTC BbvCI 2789 GCTGAGG CCTCAGC(-5/-2) 3280 GCTGAGG BclI 711 TGATCA T^GATCA 5050 TGATCA BpuAI 477 GTCTTC GAAGAC(2/6) 4771 GTCTTC BsaWI 3324 ACCGGT [AT]^CCGG[AT] 5062 TCCGGA BsmBI 1142 GAGACG CGTCTC(1/5) 3651 GAGACG BspLU11I 362 ACATGT A^CATGT 2330 ACATGT BssHII 1282 GCGCGC G^CGCGC 1356 GCGCGC BssSI 1151 CACGAG CACGAG(-5/-1) 4554 CTCGTG BstAPI 213 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3385 GCAGAAACTGC BstEII 3447 GGTGACC G^GT[ACGT]ACC 4140 GGTCACC BstPI 3447 GGTGACC G^GT[ACGT]ACC 4140 GGTCACC DraIII 75 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 2732 CACTGAGTG EcoO65I 3447 GGTGACC G^GT[ACGT]ACC 4140 GGTCACC FbaI 711 TGATCA T^GATCA 5050 TGATCA KasI 1332 GGCGCC G^GCGCC 3518 GGCGCC MluNI 627 TGGCCA TGG^CCA 4186 TGGCCA MscI 627 TGGCCA TGG^CCA 4186 TGGCCA NaeI 1363 GCCGGC GCC^GGC 2298 GCCGGC NarI 1332 GGCGCC GG^CGCC 3518 GGCGCC NgoMIV 1363 GCCGGC G^CCGGC 2298 GCCGGC NheI 1879 GCTAGC G^CTAGC 2444 GCTAGC SapI 3975 GCTCTTC GCTCTTC(1/4) 4048 GCTCTTC SfoI 1332 GGCGCC GGC^GCC 3518 GGCGCC StuI 822 AGGCCT AGG^CCT 2608 AGGCCT XcmI 2758 CCAGCCTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3494 CCACGCGGCTCCTGG XmnI 2886 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4366 GAACGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SgrAI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI