##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AflIII Aor51HI
AscI Asp700I BbrPI BcgI BsaAI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BssHII BssSI BstEII
BstPI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
HpaI KspI MluI MroI NaeI
NgoMIV NotI NruI PacI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SgrAI SnaBI SpeI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AgeI 2904 ACCGGT A^CCGGT
AhdI 5938 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5390 GTGCAC G^TGCAC
ApaLI 5390 GTGCAC G^TGCAC
AseI 2167 ATTAAT AT^TAAT
AsnI 2167 ATTAAT AT^TAAT
AspEI 5938 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4687 TGCGCA TGC^GCA
BbeI 4498 GGCGCC GGCGC^C
BciVI 582 GGATAC GTATCC(6/5)
BclI 3724 TGATCA T^GATCA
BglI 401 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3725 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2904 ACCGGT [AT]^CCGG[AT]
BsgI 2773 GTGCAG GTGCAG(16/14)
BsiEI 498 CGGTCG CG[GA][CT]^CG
BsmI 3711 GAATGC GAATGC(1/-1)
BsrDI 5341 GCAATG GCAATG(2/0)
BsrFI 2904 ACCGGT [GA]^CCGG[CT]
Bst1107I 773 GTATAC GTA^TAC
BstBI 231 TTCGAA TT^CGAA
BstZ17I 773 GTATAC GTA^TAC
Cfr10I 2904 ACCGGT [GA]^CCGG[CT]
DraIII 1561 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5938 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3022 GAATTC G^AATTC
FbaI 3724 TGATCA T^GATCA
FspI 4687 TGCGCA TGC^GCA
KasI 4498 GGCGCC G^GCGCC
MamI 3725 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4498 GGCGCC GG^CGCC
NspV 231 TTCGAA TT^CGAA
PaeR7I 5095 CTCGAG C^TCGAG
PflFI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2904 ACCGGT A^CCGGT
PshAI 650 GACGTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2167 ATTAAT AT^TAAT
SbfI 828 CCTGCAGG CCTGCA^GG
SexAI 630 ACCAGGT A^CC[AT]GGT
SfoI 4498 GGCGCC GGC^GCC
SfuI 231 TTCGAA TT^CGAA
SmaI 4117 CCCGGG CCC^GGG
Sse8387I 828 CCTGCAGG CCTGCA^GG
SwaI 6158 ATTTAAAT ATTT^AAAT
Tth111I 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5095 CTCGAG C^TCGAG
XmaCI 4117 CCCGGG C^CCGGG
XmaI 4117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1520 GGTACC G^GTACC
5760 GGTACC
AccI 773 GTATAC GT^[AC][GT]AC
2035 GTAGAC
AccII 255 CGCG CG^CG
5970 CGCG
AflII 1415 CTTAAG C^TTAAG
5790 CTTAAG
Asp718I 1520 GGTACC G^GTACC
5760 GGTACC
AvrII 488 CCTAGG C^CTAGG
578 CCTAGG
BfrI 1415 CTTAAG C^TTAAG
5790 CTTAAG
BglII 2063 AGATCT A^GATCT
3342 AGATCT
BlnI 488 CCTAGG C^CTAGG
578 CCTAGG
BsrBI 4089 GAGCGG CCGCTC(-3/-3)
4233 GAGCGG
BstUI 255 CGCG CG^CG
5970 CGCG
DrdI 541 GACCAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4809 GACATCGGAGTC
EcoT22I 1657 ATGCAT ATGCA^T
3713 ATGCAT
HincII 1319 GTTGAC GT[CT]^[GA]AC
5935 GTTGAC
HindII 1319 GTTGAC GT[CT]^[GA]AC
5935 GTTGAC
HindIII 847 AAGCTT A^AGCTT
4673 AAGCTT
KpnI 1520 GGTACC GGTAC^C
5760 GGTACC
MfeI 5540 CAATTG C^AATTG
5726 CAATTG
MunI 5540 CAATTG C^AATTG
5726 CAATTG
MvnI 255 CGCG CG^CG
5970 CGCG
NcoI 1088 CCATGG C^CATGG
4886 CCATGG
NdeI 2225 CATATG CA^TATG
2311 CATATG
NsiI 1657 ATGCAT ATGCA^T
3713 ATGCAT
PvuII 1274 CAGCTG CAG^CTG
4971 CAGCTG
SphI 358 GCATGC GCATG^C
4102 GCATGC
XcmI 5546 CCAAGGTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5907 CCACACCTGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII