##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI AocI ApaI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BcgI BglI BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV FseI FspI
Hin1I HindIII HpaI KasI KpnI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI PacI PflFI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 5174 CGCG CG^CG
AgeI 1417 ACCGGT A^CCGGT
Aor51HI 5229 AGCGCT AGC^GCT
AseI 3349 ATTAAT AT^TAAT
AsnI 3349 ATTAAT AT^TAAT
AvaI 5267 CTCGAG C^[CT]CG[GA]G
BalI 2125 TGGCCA TGG^CCA
BamHI 1915 GGATCC G^GATCC
BanI 1392 GGCACC G^G[CT][GA]CC
BbrPI 4299 CACGTG CAC^GTG
BbvCI 774 GCTGAGG CCTCAGC(-5/-2)
BciVI 3997 GTATCC GTATCC(6/5)
BclI 1696 TGATCA T^GATCA
BsmBI 4440 GAGACG CGTCTC(1/5)
BsmI 823 GAATGC GAATGC(1/-1)
BsoBI 5267 CTCGAG C^[CT]CG[GA]G
BsrFI 1417 ACCGGT [GA]^CCGG[CT]
BstBI 4730 TTCGAA TT^CGAA
BstUI 5174 CGCG CG^CG
Cfr10I 1417 ACCGGT [GA]^CCGG[CT]
EaeI 2125 TGGCCA [CT]^GGCC[GA]
Eco47III 5229 AGCGCT AGC^GCT
FbaI 1696 TGATCA T^GATCA
HgaI 1973 GACGC GACGC(5/10)
KspI 5173 CCGCGG CCGC^GG
MfeI 3280 CAATTG C^AATTG
MluNI 2125 TGGCCA TGG^CCA
MscI 2125 TGGCCA TGG^CCA
MunI 3280 CAATTG C^AATTG
MvnI 5174 CGCG CG^CG
NcoI 1953 CCATGG C^CATGG
NdeI 5025 CATATG CA^TATG
NspV 4730 TTCGAA TT^CGAA
PaeR7I 5267 CTCGAG C^TCGAG
PinAI 1417 ACCGGT A^CCGGT
PmaCI 4299 CACGTG CAC^GTG
PmlI 4299 CACGTG CAC^GTG
PshBI 3349 ATTAAT AT^TAAT
PstI 5478 CTGCAG CTGCA^G
PvuII 114 CAGCTG CAG^CTG
SacII 5173 CCGCGG CCGC^GG
SalI 5075 GTCGAC G^TCGAC
SexAI 612 ACCAGGT A^CC[AT]GGT
SfuI 4730 TTCGAA TT^CGAA
SpeI 3602 ACTAGT A^CTAGT
StuI 1379 AGGCCT AGG^CCT
XhoI 5267 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 1157 CTTAAG C^TTAAG
5321 CTTAAG
Alw44I 1612 GTGCAC G^TGCAC
4039 GTGCAC
ApaLI 1612 GTGCAC G^TGCAC
4039 GTGCAC
Asp700I 4723 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5402 GAAGAAATTC
BanII 4482 GAGCTC G[GA]GC[CT]^C
5039 GAGCTC
BfrI 1157 CTTAAG C^TTAAG
5321 CTTAAG
BsaAI 3491 CACGTA [CT]AC^GT[GA]
4299 CACGTG
BsaWI 1417 ACCGGT [AT]^CCGG[AT]
4233 ACCGGA
BspHI 1542 TCATGA T^CATGA
5277 TCATGA
CfoI 4474 GCGC GCG^C
5230 GCGC
DraII 308 AGGGCCT [GA]G^G[ACGT]CC[CT]
1352 AGGCCCT
DraIII 3336 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
5545 CACGAAGTG
DsaI 1953 CCATGG C^C[GA][CT]GG
5173 CCGCGG
EcoNI 1431 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1919 CCTTACAGAGG
EcoO109I 308 AGGGCCT [GA]G^G[ACGT]CC[CT]
1352 AGGCCCT
EcoRI 302 GAATTC G^AATTC
2252 GAATTC
EcoT14I 1953 CCATGG C^C[AT][AT]GG
3049 CCTTGG
EcoT22I 3072 ATGCAT ATGCA^T
3323 ATGCAT
HaeII 4473 GGCGCT [GA]GCGC^[CT]
5229 AGCGCT
HhaI 4474 GCGC GCG^C
5230 GCGC
HinP1I 4474 GCGC G^CGC
5230 GCGC
NsiI 3072 ATGCAT ATGCA^T
3323 ATGCAT
RcaI 1542 TCATGA T^CATGA
5277 TCATGA
SacI 4482 GAGCTC GAGCT^C
5039 GAGCTC
ScaI 4377 AGTACT AGT^ACT
5167 AGTACT
StyI 1953 CCATGG C^C[AT][AT]GG
3049 CCTTGG
XbaI 1181 TCTAGA T^CTAGA
4354 TCTAGA
XmnI 4723 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5402 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII