################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AhdI AocI ApaI AscI Asp718I AspEI AspI AviII AvrII BbeI BcgI BglI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BspDI BspEI BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FseI FspI Hin1I HindIII HpaI KasI KpnI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PacI PflFI PmeI PpuMI PshAI Psp1406I PvuI RsrII SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 5174 CGCG CG^CG AgeI 1417 ACCGGT A^CCGGT Aor51HI 5229 AGCGCT AGC^GCT AseI 3349 ATTAAT AT^TAAT AsnI 3349 ATTAAT AT^TAAT AvaI 5267 CTCGAG C^[CT]CG[GA]G BalI 2125 TGGCCA TGG^CCA BamHI 1915 GGATCC G^GATCC BanI 1392 GGCACC G^G[CT][GA]CC BbrPI 4299 CACGTG CAC^GTG BbvCI 774 GCTGAGG CCTCAGC(-5/-2) BciVI 3997 GTATCC GTATCC(6/5) BclI 1696 TGATCA T^GATCA BsmBI 4440 GAGACG CGTCTC(1/5) BsmI 823 GAATGC GAATGC(1/-1) BsoBI 5267 CTCGAG C^[CT]CG[GA]G BsrFI 1417 ACCGGT [GA]^CCGG[CT] BstBI 4730 TTCGAA TT^CGAA BstUI 5174 CGCG CG^CG Cfr10I 1417 ACCGGT [GA]^CCGG[CT] EaeI 2125 TGGCCA [CT]^GGCC[GA] Eco47III 5229 AGCGCT AGC^GCT FbaI 1696 TGATCA T^GATCA HgaI 1973 GACGC GACGC(5/10) KspI 5173 CCGCGG CCGC^GG MfeI 3280 CAATTG C^AATTG MluNI 2125 TGGCCA TGG^CCA MscI 2125 TGGCCA TGG^CCA MunI 3280 CAATTG C^AATTG MvnI 5174 CGCG CG^CG NcoI 1953 CCATGG C^CATGG NdeI 5025 CATATG CA^TATG NspV 4730 TTCGAA TT^CGAA PaeR7I 5267 CTCGAG C^TCGAG PinAI 1417 ACCGGT A^CCGGT PmaCI 4299 CACGTG CAC^GTG PmlI 4299 CACGTG CAC^GTG PshBI 3349 ATTAAT AT^TAAT PstI 5478 CTGCAG CTGCA^G PvuII 114 CAGCTG CAG^CTG SacII 5173 CCGCGG CCGC^GG SalI 5075 GTCGAC G^TCGAC SexAI 612 ACCAGGT A^CC[AT]GGT SfuI 4730 TTCGAA TT^CGAA SpeI 3602 ACTAGT A^CTAGT StuI 1379 AGGCCT AGG^CCT XhoI 5267 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflII 1157 CTTAAG C^TTAAG 5321 CTTAAG Alw44I 1612 GTGCAC G^TGCAC 4039 GTGCAC ApaLI 1612 GTGCAC G^TGCAC 4039 GTGCAC Asp700I 4723 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5402 GAAGAAATTC BanII 4482 GAGCTC G[GA]GC[CT]^C 5039 GAGCTC BfrI 1157 CTTAAG C^TTAAG 5321 CTTAAG BsaAI 3491 CACGTA [CT]AC^GT[GA] 4299 CACGTG BsaWI 1417 ACCGGT [AT]^CCGG[AT] 4233 ACCGGA BspHI 1542 TCATGA T^CATGA 5277 TCATGA CfoI 4474 GCGC GCG^C 5230 GCGC DraII 308 AGGGCCT [GA]G^G[ACGT]CC[CT] 1352 AGGCCCT DraIII 3336 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 5545 CACGAAGTG DsaI 1953 CCATGG C^C[GA][CT]GG 5173 CCGCGG EcoNI 1431 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1919 CCTTACAGAGG EcoO109I 308 AGGGCCT [GA]G^G[ACGT]CC[CT] 1352 AGGCCCT EcoRI 302 GAATTC G^AATTC 2252 GAATTC EcoT14I 1953 CCATGG C^C[AT][AT]GG 3049 CCTTGG EcoT22I 3072 ATGCAT ATGCA^T 3323 ATGCAT HaeII 4473 GGCGCT [GA]GCGC^[CT] 5229 AGCGCT HhaI 4474 GCGC GCG^C 5230 GCGC HinP1I 4474 GCGC G^CGC 5230 GCGC NsiI 3072 ATGCAT ATGCA^T 3323 ATGCAT RcaI 1542 TCATGA T^CATGA 5277 TCATGA SacI 4482 GAGCTC GAGCT^C 5039 GAGCTC ScaI 4377 AGTACT AGT^ACT 5167 AGTACT StyI 1953 CCATGG C^C[AT][AT]GG 3049 CCTTGG XbaI 1181 TCTAGA T^CTAGA 4354 TCTAGA XmnI 4723 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5402 GAAGAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII