################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I ApaI AscI AseI AsnI Asp718I AviII BbrPI BcgI BsaBI BsiEI BsiWI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I FseI FspI KpnI KspI MamI MluI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SbfI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 1364 AACGTT AA^CGTT AcyI 204 GGCGCC G[GA]^CG[CT]C AgeI 1611 ACCGGT A^CCGGT Alw44I 2428 GTGCAC G^TGCAC AocI 775 CCTAAGG CC^T[ACGT]AGG ApaLI 2428 GTGCAC G^TGCAC AspI 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 4687 CTCGGG C^[CT]CG[GA]G BalI 1534 TGGCCA TGG^CCA BamHI 2597 GGATCC G^GATCC BbeI 204 GGCGCC GGCGC^C BbvCI 3524 GCTGAGG CCTCAGC(-5/-2) BciVI 135 GGATAC GTATCC(6/5) BclI 3745 TGATCA T^GATCA BglI 3901 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2092 AGATCT A^GATCT BsaAI 4622 TACGTA [CT]AC^GT[GA] BsaHI 204 GGCGCC G[GA]^CG[CT]C BsoBI 4687 CTCGGG C^[CT]CG[GA]G BstXI 2273 CCAGCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 775 CCTAAGG CC^T[ACGT]AGG DraIII 2018 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 187 CCATGG C^C[GA][CT]GG EaeI 1534 TGGCCA [CT]^GGCC[GA] Eco81I 775 CCTAAGG CC^T[ACGT]AGG EcoRI 1073 GAATTC G^AATTC EcoRV 1958 GATATC GAT^ATC FbaI 3745 TGATCA T^GATCA Hin1I 204 GGCGCC G[GA]^CG[CT]C KasI 204 GGCGCC G^GCGCC MfeI 2038 CAATTG C^AATTG MluNI 1534 TGGCCA TGG^CCA MscI 1534 TGGCCA TGG^CCA MunI 2038 CAATTG C^AATTG NarI 204 GGCGCC GG^CGCC NcoI 187 CCATGG C^CATGG NheI 1597 GCTAGC G^CTAGC PflFI 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1611 ACCGGT A^CCGGT PpuMI 70 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 4446 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1364 AACGTT AA^CGTT SalI 121 GTCGAC G^TCGAC SfoI 204 GGCGCC GGC^GCC SnaBI 4622 TACGTA TAC^GTA StuI 1164 AGGCCT AGG^CCT SwaI 4161 ATTTAAAT ATTT^AAAT Tth111I 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1297 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 2778 CGCG CG^CG 3376 CGCG AflII 1168 CTTAAG C^TTAAG 4448 CTTAAG Aor51HI 3303 AGCGCT AGC^GCT 3359 AGCGCT Asp700I 2048 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2453 GAAGAGCTTC AvrII 868 CCTAGG C^CTAGG 4419 CCTAGG BbsI 1 GAAGAC GAAGAC(2/6) 3038 GAAGAC BfrI 1168 CTTAAG C^TTAAG 4448 CTTAAG BlnI 868 CCTAGG C^CTAGG 4419 CCTAGG BlpI 1045 GCTCAGC GC^T[ACGT]AGC 3728 GCTCAGC Bpu1102I 1045 GCTCAGC GC^T[ACGT]AGC 3728 GCTCAGC BpuAI 1 GAAGAC GAAGAC(2/6) 3038 GAAGAC BseRI 1703 CTCCTC GAGGAG(10/8) 1784 GAGGAG BsmBI 78 GAGACG CGTCTC(1/5) 1787 GAGACG Bsp1407I 178 TGTACA T^GTACA 387 TGTACA BsrBI 365 GAGCGG CCGCTC(-3/-3) 2705 GAGCGG BsrFI 1611 ACCGGT [GA]^CCGG[CT] 1899 ACCGGC BsrGI 178 TGTACA T^GTACA 387 TGTACA BstUI 2778 CGCG CG^CG 3376 CGCG CelII 1045 GCTCAGC GC^T[ACGT]AGC 3728 GCTCAGC Cfr10I 1611 ACCGGT [GA]^CCGG[CT] 1899 ACCGGC Eco47III 3303 AGCGCT AGC^GCT 3359 AGCGCT EcoNI 1705 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3508 CCTACTTCAGG HgaI 2010 GACGC GACGC(5/10) 3621 GACGC MvnI 2778 CGCG CG^CG 3376 CGCG ScaI 630 AGTACT AGT^ACT 3968 AGTACT SspBI 178 TGTACA T^GTACA 387 TGTACA SspI 4258 AATATT AAT^ATT 4334 AATATT XmnI 2048 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2453 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AccIII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI ApoI AspEI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsaWI BseAI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspEI BspHI BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII Eam1105I EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII