##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I ApaI AscI AseI
AsnI Asp718I AviII BbrPI BcgI
BsaBI BsiEI BsiWI BspDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I FseI FspI
KpnI KspI MamI MluI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SmaI SpeI Sse8387I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 1364 AACGTT AA^CGTT
AcyI 204 GGCGCC G[GA]^CG[CT]C
AgeI 1611 ACCGGT A^CCGGT
Alw44I 2428 GTGCAC G^TGCAC
AocI 775 CCTAAGG CC^T[ACGT]AGG
ApaLI 2428 GTGCAC G^TGCAC
AspI 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4687 CTCGGG C^[CT]CG[GA]G
BalI 1534 TGGCCA TGG^CCA
BamHI 2597 GGATCC G^GATCC
BbeI 204 GGCGCC GGCGC^C
BbvCI 3524 GCTGAGG CCTCAGC(-5/-2)
BciVI 135 GGATAC GTATCC(6/5)
BclI 3745 TGATCA T^GATCA
BglI 3901 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2092 AGATCT A^GATCT
BsaAI 4622 TACGTA [CT]AC^GT[GA]
BsaHI 204 GGCGCC G[GA]^CG[CT]C
BsoBI 4687 CTCGGG C^[CT]CG[GA]G
BstXI 2273 CCAGCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 775 CCTAAGG CC^T[ACGT]AGG
DraIII 2018 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 187 CCATGG C^C[GA][CT]GG
EaeI 1534 TGGCCA [CT]^GGCC[GA]
Eco81I 775 CCTAAGG CC^T[ACGT]AGG
EcoRI 1073 GAATTC G^AATTC
EcoRV 1958 GATATC GAT^ATC
FbaI 3745 TGATCA T^GATCA
Hin1I 204 GGCGCC G[GA]^CG[CT]C
KasI 204 GGCGCC G^GCGCC
MfeI 2038 CAATTG C^AATTG
MluNI 1534 TGGCCA TGG^CCA
MscI 1534 TGGCCA TGG^CCA
MunI 2038 CAATTG C^AATTG
NarI 204 GGCGCC GG^CGCC
NcoI 187 CCATGG C^CATGG
NheI 1597 GCTAGC G^CTAGC
PflFI 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1611 ACCGGT A^CCGGT
PpuMI 70 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4446 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1364 AACGTT AA^CGTT
SalI 121 GTCGAC G^TCGAC
SfoI 204 GGCGCC GGC^GCC
SnaBI 4622 TACGTA TAC^GTA
StuI 1164 AGGCCT AGG^CCT
SwaI 4161 ATTTAAAT ATTT^AAAT
Tth111I 2767 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1297 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2778 CGCG CG^CG
3376 CGCG
AflII 1168 CTTAAG C^TTAAG
4448 CTTAAG
Aor51HI 3303 AGCGCT AGC^GCT
3359 AGCGCT
Asp700I 2048 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2453 GAAGAGCTTC
AvrII 868 CCTAGG C^CTAGG
4419 CCTAGG
BbsI 1 GAAGAC GAAGAC(2/6)
3038 GAAGAC
BfrI 1168 CTTAAG C^TTAAG
4448 CTTAAG
BlnI 868 CCTAGG C^CTAGG
4419 CCTAGG
BlpI 1045 GCTCAGC GC^T[ACGT]AGC
3728 GCTCAGC
Bpu1102I 1045 GCTCAGC GC^T[ACGT]AGC
3728 GCTCAGC
BpuAI 1 GAAGAC GAAGAC(2/6)
3038 GAAGAC
BseRI 1703 CTCCTC GAGGAG(10/8)
1784 GAGGAG
BsmBI 78 GAGACG CGTCTC(1/5)
1787 GAGACG
Bsp1407I 178 TGTACA T^GTACA
387 TGTACA
BsrBI 365 GAGCGG CCGCTC(-3/-3)
2705 GAGCGG
BsrFI 1611 ACCGGT [GA]^CCGG[CT]
1899 ACCGGC
BsrGI 178 TGTACA T^GTACA
387 TGTACA
BstUI 2778 CGCG CG^CG
3376 CGCG
CelII 1045 GCTCAGC GC^T[ACGT]AGC
3728 GCTCAGC
Cfr10I 1611 ACCGGT [GA]^CCGG[CT]
1899 ACCGGC
Eco47III 3303 AGCGCT AGC^GCT
3359 AGCGCT
EcoNI 1705 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3508 CCTACTTCAGG
HgaI 2010 GACGC GACGC(5/10)
3621 GACGC
MvnI 2778 CGCG CG^CG
3376 CGCG
ScaI 630 AGTACT AGT^ACT
3968 AGTACT
SspBI 178 TGTACA T^GTACA
387 TGTACA
SspI 4258 AATATT AAT^ATT
4334 AATATT
XmnI 2048 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2453 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseAI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspEI BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII Eam1105I EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII