##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AvrII BfrI
BglII BlnI BsiWI BspDI Bst1107I
BstBI BstZ17I ClaI DraI EcoRI
EcoRV FseI HpaI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I SalI SfiI
SfuI SgrAI SnaBI SphI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2331 GGTACC G^GTACC
AccI 1304 GTAGAC GT^[AC][GT]AC
AccIII 3597 TCCGGA T^CCGGA
Aor51HI 2217 AGCGCT AGC^GCT
Asp718I 2331 GGTACC G^GTACC
AviII 1371 TGCGCA TGC^GCA
BclI 2071 TGATCA T^GATCA
BsaBI 1708 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3597 TCCGGA T^CCGGA
Bsp1407I 1710 TGTACA T^GTACA
BspEI 3597 TCCGGA T^CCGGA
BspLU11I 5611 ACATGT A^CATGT
BsrDI 1795 CATTGC GCAATG(2/0)
BsrGI 1710 TGTACA T^GTACA
BssHII 463 GCGCGC G^CGCGC
DraIII 1763 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3996 GACCCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 33 CGGCCG C^GGCCG
EclXI 33 CGGCCG C^GGCCG
Eco47III 2217 AGCGCT AGC^GCT
Eco52I 33 CGGCCG C^GGCCG
EcoNI 1231 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3536 ATGCAT ATGCA^T
FbaI 2071 TGATCA T^GATCA
FspI 1371 TGCGCA TGC^GCA
HindIII 656 AAGCTT A^AGCTT
KpnI 2331 GGTACC GGTAC^C
MamI 1708 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3597 TCCGGA T^CCGGA
NsiI 3536 ATGCAT ATGCA^T
PvuI 310 CGATCG CGAT^CG
SacI 739 GAGCTC GAGCT^C
SapI 1757 GAAGAGC GCTCTTC(1/4)
SpeI 2318 ACTAGT A^CTAGT
SspBI 1710 TGTACA T^GTACA
StuI 3870 AGGCCT AGG^CCT
Tsp509I 4294 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 1106 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3033 GACTGGTGGTC
AspEI 1106 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3033 GACTGGTGGTC
AspI 1199 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2294 GACTGTGTC
BamHI 3729 GGATCC G^GATCC
4509 GGATCC
BcgI 467 GCACAGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
486 GCACGTCGTTCG
BsiEI 33 CGGCCG CG[GA][CT]^CG
310 CGATCG
BsmBI 507 GAGACG CGTCTC(1/5)
1931 GAGACG
BsmI 4198 GAATGC GAATGC(1/-1)
3365 GCATTC
BsrBI 24 CCGCTC CCGCTC(-3/-3)
1969 CCGCTC
BsrFI 436 GCCGGT [GA]^CCGG[CT]
443 GCCGGT
BssSI 330 CACGAG CACGAG(-5/-1)
3528 CTCGTG
BstXI 170 CCACGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCAGGGAGCTGG
Cfr10I 436 GCCGGT [GA]^CCGG[CT]
443 GCCGGT
CpoI 132 CGGACCG CG^G[AT]CCG
361 CGGTCCG
Eam1105I 1106 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3033 GACTGGTGGTC
NcoI 78 CCATGG C^CATGG
3724 CCATGG
PflFI 1199 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2294 GACTGTGTC
RsrII 132 CGGACCG CG^G[AT]CCG
361 CGGTCCG
SbfI 2425 CCTGCAGG CCTGCA^GG
2695 CCTGCAGG
ScaI 579 AGTACT AGT^ACT
1173 AGTACT
Sse8387I 2425 CCTGCAGG CCTGCA^GG
2695 CCTGCAGG
Tth111I 1199 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2294 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RcaI RsaI SacII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI