##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccII AflII AflIII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BciVI BfrI BlnI BsaBI
BspDI BspLU11I BssHII BstBI BstEII
BstPI BstUI ClaI CpoI EcoO65I
EcoT22I FseI FspI HpaI KpnI
KspI MamI MfeI MluI MunI
MvnI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 933 AACGTT AA^CGTT
AhdI 5158 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5158 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1500 GGCGCC GGCGC^C
BbvCI 3122 GCTGAGG CCTCAGC(-5/-2)
BglI 2246 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4636 AGATCT A^GATCT
BsiWI 1557 CGTACG C^GTACG
Bsp1407I 5687 TGTACA T^GTACA
BspHI 3583 TCATGA T^CATGA
BsrBI 3497 GAGCGG CCGCTC(-3/-3)
BsrDI 5547 CATTGC GCAATG(2/0)
BsrGI 5687 TGTACA T^GTACA
Bst1107I 2791 GTATAC GTA^TAC
BstZ17I 2791 GTATAC GTA^TAC
EagI 2748 CGGCCG C^GGCCG
Eam1105I 5158 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2748 CGGCCG C^GGCCG
Eco52I 2748 CGGCCG C^GGCCG
HindIII 994 AAGCTT A^AGCTT
KasI 1500 GGCGCC G^GCGCC
NaeI 1695 GCCGGC GCC^GGC
NarI 1500 GGCGCC GG^CGCC
NgoMIV 1695 GCCGGC G^CCGGC
NheI 4378 GCTAGC G^CTAGC
PaeR7I 2898 CTCGAG C^TCGAG
PshAI 3514 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 933 AACGTT AA^CGTT
RcaI 3583 TCATGA T^CATGA
ScaI 106 AGTACT AGT^ACT
SexAI 2393 ACCTGGT A^CC[AT]GGT
SfoI 1500 GGCGCC GGC^GCC
SspBI 5687 TGTACA T^GTACA
SspI 5449 AATATT AAT^ATT
XhoI 2898 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 4666 TCCGGA T^CCGGA
5243 TCCGGA
Asp700I 869 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3145 GAACGGCTTC
BalI 3912 TGGCCA TGG^CCA
5792 TGGCCA
BbrPI 896 CACGTG CAC^GTG
4589 CACGTG
BcgI 2670 GCATGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5580 GCATGGATGTCG
BsaWI 4666 TCCGGA [AT]^CCGG[AT]
5243 TCCGGA
BseAI 4666 TCCGGA T^CCGGA
5243 TCCGGA
BsmI 3054 GCATTC GAATGC(1/-1)
5207 GCATTC
BspEI 4666 TCCGGA T^CCGGA
5243 TCCGGA
DraIII 3470 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5281 CACACTGTG
DrdI 910 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4433 GACCGCCAGGTC
EcoRI 1304 GAATTC G^AATTC
1619 GAATTC
EcoRV 551 GATATC GAT^ATC
2501 GATATC
HincII 5040 GTTGAC GT[CT]^[GA]AC
5837 GTCAAC
HindII 5040 GTTGAC GT[CT]^[GA]AC
5837 GTCAAC
MluNI 3912 TGGCCA TGG^CCA
5792 TGGCCA
MroI 4666 TCCGGA T^CCGGA
5243 TCCGGA
MscI 3912 TGGCCA TGG^CCA
5792 TGGCCA
NspI 3724 GCATGT [GA]CATG^[CT]
4411 GCATGT
PmaCI 896 CACGTG CAC^GTG
4589 CACGTG
PmlI 896 CACGTG CAC^GTG
4589 CACGTG
SacI 2417 GAGCTC GAGCT^C
2867 GAGCTC
SapI 1519 GAAGAGC GCTCTTC(1/4)
5272 GAAGAGC
XmnI 869 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3145 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII AccI AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI