##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BbeI BbsI BclI BfrI BpuAI
BsaI BsaWI BseAI BsiEI BsiWI
BspEI BsrBI BsrFI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FbaI FseI FspI HgaI
KasI KpnI Ksp632I KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfoI SgrAI SpeI Sse8387I
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2582 GTAGAC GT^[AC][GT]AC
ApaI 137 GGGCCC GGGCC^C
AspI 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 67 GGACC G^G[AT]CC
AvrII 356 CCTAGG C^CTAGG
BbrPI 163 CACGTG CAC^GTG
BcgI 1160 GCACATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4390 GTATCC GTATCC(6/5)
BlnI 356 CCTAGG C^CTAGG
BlpI 1419 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1419 GCTTAGC GC^T[ACGT]AGC
BsmBI 200 CGTCTC CGTCTC(1/5)
BsmI 3510 GCATTC GAATGC(1/-1)
BspDI 5051 ATCGAT AT^CGAT
BssHII 2682 GCGCGC G^CGCGC
BstBI 1168 TTCGAA TT^CGAA
BstXI 3630 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1419 GCTTAGC GC^T[ACGT]AGC
ClaI 5051 ATCGAT AT^CGAT
DrdI 1691 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 42 GGCGCT [GA]GCGC^[CT]
HindIII 1417 AAGCTT A^AGCTT
NdeI 4099 CATATG CA^TATG
NruI 108 TCGCGA TCG^CGA
NspV 1168 TTCGAA TT^CGAA
PacI 3389 TTAATTAA TTAAT^TAA
PflFI 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 163 CACGTG CAC^GTG
PmlI 163 CACGTG CAC^GTG
ScaI 2934 AGTACT AGT^ACT
SexAI 1803 ACCAGGT A^CC[AT]GGT
SfiI 1330 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1168 TTCGAA TT^CGAA
SmaI 125 CCCGGG CCC^GGG
SphI 2686 GCATGC GCATG^C
Tth111I 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 198 GACGTC GACGT^C
2261 GACGTC
AccII 109 CGCG CG^CG
2683 CGCG
AcyI 198 GACGTC G[GA]^CG[CT]C
2261 GACGTC
Alw44I 2054 GTGCAC G^TGCAC
2645 GTGCAC
ApaLI 2054 GTGCAC G^TGCAC
2645 GTGCAC
Asp700I 215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3525 GAATGGATTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
1737 CTCGGG
BalI 1329 TGGCCA TGG^CCA
3001 TGGCCA
BanII 137 GGGCCC G[GA]GC[CT]^C
620 GGGCTC
BbvCI 2311 CCTCAGC CCTCAGC(-5/-2)
2420 GCTGAGG
BsaBI 4349 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5050 GATCGATATC
BsaHI 198 GACGTC G[GA]^CG[CT]C
2261 GACGTC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
1737 CTCGGG
BspHI 2845 TCATGA T^CATGA
4865 TCATGA
BstUI 109 CGCG CG^CG
2683 CGCG
EcoRI 1857 GAATTC G^AATTC
3543 GAATTC
Hin1I 198 GACGTC G[GA]^CG[CT]C
2261 GACGTC
HpaI 2341 GTTAAC GTT^AAC
4120 GTTAAC
MamI 4349 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5050 GATCGATATC
MluNI 1329 TGGCCA TGG^CCA
3001 TGGCCA
MscI 1329 TGGCCA TGG^CCA
3001 TGGCCA
MvnI 109 CGCG CG^CG
2683 CGCG
RcaI 2845 TCATGA T^CATGA
4865 TCATGA
SnaBI 2496 TACGTA TAC^GTA
4523 TACGTA
XcmI 625 CCAAATTGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1180 CCAAGTTGAAAATGG
XmnI 215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3525 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI BanI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII