################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BamHI BbeI BbsI BclI BfrI BpuAI BsaI BsaWI BseAI BsiEI BsiWI BspEI BsrBI BsrFI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FbaI FseI FspI HgaI KasI KpnI Ksp632I KspI MluI MroI NaeI NarI NgoMIV NheI NotI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfoI SgrAI SpeI Sse8387I SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 2582 GTAGAC GT^[AC][GT]AC ApaI 137 GGGCCC GGGCC^C AspI 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 67 GGACC G^G[AT]CC AvrII 356 CCTAGG C^CTAGG BbrPI 163 CACGTG CAC^GTG BcgI 1160 GCACATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4390 GTATCC GTATCC(6/5) BlnI 356 CCTAGG C^CTAGG BlpI 1419 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1419 GCTTAGC GC^T[ACGT]AGC BsmBI 200 CGTCTC CGTCTC(1/5) BsmI 3510 GCATTC GAATGC(1/-1) BspDI 5051 ATCGAT AT^CGAT BssHII 2682 GCGCGC G^CGCGC BstBI 1168 TTCGAA TT^CGAA BstXI 3630 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1419 GCTTAGC GC^T[ACGT]AGC ClaI 5051 ATCGAT AT^CGAT DrdI 1691 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 42 GGCGCT [GA]GCGC^[CT] HindIII 1417 AAGCTT A^AGCTT NdeI 4099 CATATG CA^TATG NruI 108 TCGCGA TCG^CGA NspV 1168 TTCGAA TT^CGAA PacI 3389 TTAATTAA TTAAT^TAA PflFI 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 163 CACGTG CAC^GTG PmlI 163 CACGTG CAC^GTG ScaI 2934 AGTACT AGT^ACT SexAI 1803 ACCAGGT A^CC[AT]GGT SfiI 1330 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1168 TTCGAA TT^CGAA SmaI 125 CCCGGG CCC^GGG SphI 2686 GCATGC GCATG^C Tth111I 2258 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 125 CCCGGG C^CCGGG XmaI 125 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 198 GACGTC GACGT^C 2261 GACGTC AccII 109 CGCG CG^CG 2683 CGCG AcyI 198 GACGTC G[GA]^CG[CT]C 2261 GACGTC Alw44I 2054 GTGCAC G^TGCAC 2645 GTGCAC ApaLI 2054 GTGCAC G^TGCAC 2645 GTGCAC Asp700I 215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3525 GAATGGATTC AvaI 125 CCCGGG C^[CT]CG[GA]G 1737 CTCGGG BalI 1329 TGGCCA TGG^CCA 3001 TGGCCA BanII 137 GGGCCC G[GA]GC[CT]^C 620 GGGCTC BbvCI 2311 CCTCAGC CCTCAGC(-5/-2) 2420 GCTGAGG BsaBI 4349 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5050 GATCGATATC BsaHI 198 GACGTC G[GA]^CG[CT]C 2261 GACGTC BsoBI 125 CCCGGG C^[CT]CG[GA]G 1737 CTCGGG BspHI 2845 TCATGA T^CATGA 4865 TCATGA BstUI 109 CGCG CG^CG 2683 CGCG EcoRI 1857 GAATTC G^AATTC 3543 GAATTC Hin1I 198 GACGTC G[GA]^CG[CT]C 2261 GACGTC HpaI 2341 GTTAAC GTT^AAC 4120 GTTAAC MamI 4349 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5050 GATCGATATC MluNI 1329 TGGCCA TGG^CCA 3001 TGGCCA MscI 1329 TGGCCA TGG^CCA 3001 TGGCCA MvnI 109 CGCG CG^CG 2683 CGCG RcaI 2845 TCATGA T^CATGA 4865 TCATGA SnaBI 2496 TACGTA TAC^GTA 4523 TACGTA XcmI 625 CCAAATTGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1180 CCAAGTTGAAAATGG XmnI 215 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3525 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI BanI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EcoO109I EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII