##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AcyI Aor51HI
AscI AspI BbeI BlpI Bpu1102I
BsaHI BseAI BsiEI BspEI BspHI
BstBI CelII CpoI EagI EclXI
Eco47III Eco52I EcoT22I FseI Hin1I
KasI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1508 GGTACC G^GTACC
AflII 5097 CTTAAG C^TTAAG
AgeI 1511 ACCGGT A^CCGGT
AhdI 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 438 GTGCAC G^TGCAC
ApaLI 438 GTGCAC G^TGCAC
Asp718I 1508 GGTACC G^GTACC
AspEI 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 919 TGCGCA TGC^GCA
AvrII 1969 CCTAGG C^CTAGG
BamHI 1631 GGATCC G^GATCC
BbvCI 2816 CCTCAGC CCTCAGC(-5/-2)
BcgI 1628 GCAGGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4782 TGATCA T^GATCA
BfrI 5097 CTTAAG C^TTAAG
BlnI 1969 CCTAGG C^CTAGG
BsaBI 3750 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1752 CGTACG C^GTACG
BspDI 2843 ATCGAT AT^CGAT
BspLU11I 3566 ACATGT A^CATGT
BssHII 1480 GCGCGC G^CGCGC
Bst1107I 2742 GTATAC GTA^TAC
BstEII 1596 GGTCACC G^GT[ACGT]ACC
BstPI 1596 GGTCACC G^GT[ACGT]ACC
BstXI 4579 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2742 GTATAC GTA^TAC
ClaI 2843 ATCGAT AT^CGAT
DrdI 546 GACCTATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 907 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1596 GGTCACC G^GT[ACGT]ACC
FbaI 4782 TGATCA T^GATCA
FspI 919 TGCGCA TGC^GCA
HpaI 5062 GTTAAC GTT^AAC
KpnI 1508 GGTACC GGTAC^C
MamI 3750 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 5071 CCATGG C^CATGG
PflMI 1352 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1511 ACCGGT A^CCGGT
PshAI 2160 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 866 GAGCTC GAGCT^C
SspI 3156 AATATT AAT^ATT
SwaI 4673 ATTTAAAT ATTT^AAAT
Van91I 1352 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 758 CCACAGCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2742 GTATAC GT^[AC][GT]AC
5055 GTAGAC
AocI 381 CCTGAGG CC^T[ACGT]AGG
2657 CCTAAGG
ApaI 120 GGGCCC GGGCC^C
1443 GGGCCC
AseI 889 ATTAAT AT^TAAT
3331 ATTAAT
AsnI 889 ATTAAT AT^TAAT
3331 ATTAAT
Asp700I 144 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
975 GAACACATTC
BalI 732 TGGCCA TGG^CCA
4860 TGGCCA
BciVI 3188 GGATAC GTATCC(6/5)
1612 GTATCC
BglII 356 AGATCT A^GATCT
4952 AGATCT
BsaI 1959 GAGACC GGTCTC(1/5)
3661 GGTCTC
BsaWI 1082 TCCGGT [AT]^CCGG[AT]
1511 ACCGGT
BseRI 1773 GAGGAG GAGGAG(10/8)
3211 GAGGAG
BsmI 452 GCATTC GAATGC(1/-1)
4422 GCATTC
BstAPI 3926 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4219 GCAGCTGGTGC
Bsu36I 381 CCTGAGG CC^T[ACGT]AGG
2657 CCTAAGG
Eco81I 381 CCTGAGG CC^T[ACGT]AGG
2657 CCTAAGG
EcoRI 1099 GAATTC G^AATTC
4989 GAATTC
EcoRV 181 GATATC GAT^ATC
2551 GATATC
HgaI 3971 GACGC GACGC(5/10)
4728 GACGC
HincII 4725 GTTGAC GT[CT]^[GA]AC
5062 GTTAAC
HindII 4725 GTTGAC GT[CT]^[GA]AC
5062 GTTAAC
HindIII 631 AAGCTT A^AGCTT
4103 AAGCTT
KspI 855 CCGCGG CCGC^GG
1291 CCGCGG
MluNI 732 TGGCCA TGG^CCA
4860 TGGCCA
MscI 732 TGGCCA TGG^CCA
4860 TGGCCA
PshBI 889 ATTAAT AT^TAAT
3331 ATTAAT
SacII 855 CCGCGG CCGC^GG
1291 CCGCGG
SbfI 1104 CCTGCAGG CCTGCA^GG
1304 CCTGCAGG
SmaI 797 CCCGGG CCC^GGG
1504 CCCGGG
SmlI 715 CTCAAG C^T[CT][GA]AG
5097 CTTAAG
SpeI 3181 ACTAGT A^CTAGT
4172 ACTAGT
Sse8387I 1104 CCTGCAGG CCTGCA^GG
1304 CCTGCAGG
XmaCI 797 CCCGGG C^CCGGG
1504 CCCGGG
XmaI 797 CCCGGG C^CCGGG
1504 CCCGGG
XmnI 144 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
975 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII