##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII Alw44I Aor51HI
ApaLI AscI Asp718I AspI AvaI
BbeI BbrPI BcgI BcnI BglII
BsaBI BseAI BsiEI BsiWI BsoBI
BspEI BspLU11I BsrBI BssHII BstBI
BstXI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HaeII
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 4961 CGCG CG^CG
AcyI 772 GACGCC G[GA]^CG[CT]C
AflII 2152 CTTAAG C^TTAAG
AflIII 4874 ACACGT A^C[GA][CT]GT
AgeI 81 ACCGGT A^CCGGT
AhdI 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5247 CCTAAGG CC^T[ACGT]AGG
ApaI 1375 GGGCCC GGGCC^C
Asp700I 4042 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4404 TGCGCA TGC^GCA
BamHI 2126 GGATCC G^GATCC
BanI 4895 GGTGCC G^G[CT][GA]CC
BciVI 2288 GTATCC GTATCC(6/5)
BfrI 2152 CTTAAG C^TTAAG
BsaHI 772 GACGCC G[GA]^CG[CT]C
BsaWI 81 ACCGGT [AT]^CCGG[AT]
BsmBI 770 GAGACG CGTCTC(1/5)
BsmI 1477 GAATGC GAATGC(1/-1)
BspDI 2448 ATCGAT AT^CGAT
BsrFI 81 ACCGGT [GA]^CCGG[CT]
Bst1107I 3261 GTATAC GTA^TAC
BstAPI 4980 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3029 GGTGACC G^GT[ACGT]ACC
BstPI 3029 GGTGACC G^GT[ACGT]ACC
BstUI 4961 CGCG CG^CG
BstZ17I 3261 GTATAC GTA^TAC
Bsu36I 5247 CCTAAGG CC^T[ACGT]AGG
CfoI 4405 GCGC GCG^C
Cfr10I 81 ACCGGT [GA]^CCGG[CT]
ClaI 2448 ATCGAT AT^CGAT
DraIII 4167 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 535 GACCTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4960 CCGCGG C^C[GA][CT]GG
Eam1105I 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5247 CCTAAGG CC^T[ACGT]AGG
EcoNI 1806 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3029 GGTGACC G^GT[ACGT]ACC
FspI 4404 TGCGCA TGC^GCA
HapII 82 CCGG C^CGG
HgaI 772 GACGC GACGC(5/10)
HhaI 4405 GCGC GCG^C
Hin1I 772 GACGCC G[GA]^CG[CT]C
HinP1I 4405 GCGC G^CGC
HpaII 82 CCGG C^CGG
KspI 4960 CCGCGG CCGC^GG
MspI 82 CCGG C^CGG
MvnI 4961 CGCG CG^CG
NdeI 1050 CATATG CA^TATG
PinAI 81 ACCGGT A^CCGGT
PpuMI 954 AGGACCC [GA]G^G[AT]CC[CT]
SacII 4960 CCGCGG CCGC^GG
SnaBI 2111 TACGTA TAC^GTA
StuI 188 AGGCCT AGG^CCT
XbaI 2742 TCTAGA T^CTAGA
XmnI 4042 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3261 GTATAC GT^[AC][GT]AC
4527 GTAGAC
AclI 2116 AACGTT AA^CGTT
4475 AACGTT
AvrII 89 CCTAGG C^CTAGG
1811 CCTAGG
BalI 1104 TGGCCA TGG^CCA
2340 TGGCCA
BglI 3098 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4513 GCCAGAAAGGC
BlnI 89 CCTAGG C^CTAGG
1811 CCTAGG
BlpI 1304 GCTCAGC GC^T[ACGT]AGC
4968 GCTCAGC
Bpu1102I 1304 GCTCAGC GC^T[ACGT]AGC
4968 GCTCAGC
BseRI 2091 CTCCTC GAGGAG(10/8)
3598 CTCCTC
BspHI 3913 TCATGA T^CATGA
5223 TCATGA
BssSI 3071 CACGAG CACGAG(-5/-1)
4696 CTCGTG
CelII 1304 GCTCAGC GC^T[ACGT]AGC
4968 GCTCAGC
EaeI 1104 TGGCCA [CT]^GGCC[GA]
2340 TGGCCA
FauI 3593 CCCGC CCCGC(4/6)
4786 CCCGC
HpaI 2814 GTTAAC GTT^AAC
2984 GTTAAC
MluNI 1104 TGGCCA TGG^CCA
2340 TGGCCA
MscI 1104 TGGCCA TGG^CCA
2340 TGGCCA
Psp1406I 2116 AACGTT AA^CGTT
4475 AACGTT
PstI 2547 CTGCAG CTGCA^G
4978 CTGCAG
PvuII 3042 CAGCTG CAG^CTG
4975 CAGCTG
RcaI 3913 TCATGA T^CATGA
5223 TCATGA
SmlI 2085 CTCAAG C^T[CT][GA]AG
2152 CTTAAG
SpeI 3513 ACTAGT A^CTAGT
5303 ACTAGT
SphI 3450 GCATGC GCATG^C
4401 GCATGC
XcmI 755 CCAAGTAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
999 CCAACCAACCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvCI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII