################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII Alw44I Aor51HI ApaLI AscI Asp718I AspI AvaI BbeI BbrPI BcgI BcnI BglII BsaBI BseAI BsiEI BsiWI BsoBI BspEI BspLU11I BsrBI BssHII BstBI BstXI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HaeII KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 4961 CGCG CG^CG AcyI 772 GACGCC G[GA]^CG[CT]C AflII 2152 CTTAAG C^TTAAG AflIII 4874 ACACGT A^C[GA][CT]GT AgeI 81 ACCGGT A^CCGGT AhdI 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 5247 CCTAAGG CC^T[ACGT]AGG ApaI 1375 GGGCCC GGGCC^C Asp700I 4042 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4404 TGCGCA TGC^GCA BamHI 2126 GGATCC G^GATCC BanI 4895 GGTGCC G^G[CT][GA]CC BciVI 2288 GTATCC GTATCC(6/5) BfrI 2152 CTTAAG C^TTAAG BsaHI 772 GACGCC G[GA]^CG[CT]C BsaWI 81 ACCGGT [AT]^CCGG[AT] BsmBI 770 GAGACG CGTCTC(1/5) BsmI 1477 GAATGC GAATGC(1/-1) BspDI 2448 ATCGAT AT^CGAT BsrFI 81 ACCGGT [GA]^CCGG[CT] Bst1107I 3261 GTATAC GTA^TAC BstAPI 4980 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3029 GGTGACC G^GT[ACGT]ACC BstPI 3029 GGTGACC G^GT[ACGT]ACC BstUI 4961 CGCG CG^CG BstZ17I 3261 GTATAC GTA^TAC Bsu36I 5247 CCTAAGG CC^T[ACGT]AGG CfoI 4405 GCGC GCG^C Cfr10I 81 ACCGGT [GA]^CCGG[CT] ClaI 2448 ATCGAT AT^CGAT DraIII 4167 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 535 GACCTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 4960 CCGCGG C^C[GA][CT]GG Eam1105I 2411 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 5247 CCTAAGG CC^T[ACGT]AGG EcoNI 1806 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3029 GGTGACC G^GT[ACGT]ACC FspI 4404 TGCGCA TGC^GCA HapII 82 CCGG C^CGG HgaI 772 GACGC GACGC(5/10) HhaI 4405 GCGC GCG^C Hin1I 772 GACGCC G[GA]^CG[CT]C HinP1I 4405 GCGC G^CGC HpaII 82 CCGG C^CGG KspI 4960 CCGCGG CCGC^GG MspI 82 CCGG C^CGG MvnI 4961 CGCG CG^CG NdeI 1050 CATATG CA^TATG PinAI 81 ACCGGT A^CCGGT PpuMI 954 AGGACCC [GA]G^G[AT]CC[CT] SacII 4960 CCGCGG CCGC^GG SnaBI 2111 TACGTA TAC^GTA StuI 188 AGGCCT AGG^CCT XbaI 2742 TCTAGA T^CTAGA XmnI 4042 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 3261 GTATAC GT^[AC][GT]AC 4527 GTAGAC AclI 2116 AACGTT AA^CGTT 4475 AACGTT AvrII 89 CCTAGG C^CTAGG 1811 CCTAGG BalI 1104 TGGCCA TGG^CCA 2340 TGGCCA BglI 3098 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4513 GCCAGAAAGGC BlnI 89 CCTAGG C^CTAGG 1811 CCTAGG BlpI 1304 GCTCAGC GC^T[ACGT]AGC 4968 GCTCAGC Bpu1102I 1304 GCTCAGC GC^T[ACGT]AGC 4968 GCTCAGC BseRI 2091 CTCCTC GAGGAG(10/8) 3598 CTCCTC BspHI 3913 TCATGA T^CATGA 5223 TCATGA BssSI 3071 CACGAG CACGAG(-5/-1) 4696 CTCGTG CelII 1304 GCTCAGC GC^T[ACGT]AGC 4968 GCTCAGC EaeI 1104 TGGCCA [CT]^GGCC[GA] 2340 TGGCCA FauI 3593 CCCGC CCCGC(4/6) 4786 CCCGC HpaI 2814 GTTAAC GTT^AAC 2984 GTTAAC MluNI 1104 TGGCCA TGG^CCA 2340 TGGCCA MscI 1104 TGGCCA TGG^CCA 2340 TGGCCA Psp1406I 2116 AACGTT AA^CGTT 4475 AACGTT PstI 2547 CTGCAG CTGCA^G 4978 CTGCAG PvuII 3042 CAGCTG CAG^CTG 4975 CAGCTG RcaI 3913 TCATGA T^CATGA 5223 TCATGA SmlI 2085 CTCAAG C^T[CT][GA]AG 2152 CTTAAG SpeI 3513 ACTAGT A^CTAGT 5303 ACTAGT SphI 3450 GCATGC GCATG^C 4401 GCATGC XcmI 755 CCAAGTAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 999 CCAACCAACCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanII BbsI BbvCI BbvI BclI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII