##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BlnI BsiWI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraIII Eam1105I
EcoRV EcoT22I FseI FspI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I RsrII SalI
SbfI ScaI SfuI SnaBI SpeI
Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 907 CTTAAG C^TTAAG
AocI 5723 CCTGAGG CC^T[ACGT]AGG
AseI 3297 ATTAAT AT^TAAT
AsnI 3297 ATTAAT AT^TAAT
AspI 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 273 GGCGCC GGCGC^C
BbvCI 1811 CCTCAGC CCTCAGC(-5/-2)
BfrI 907 CTTAAG C^TTAAG
Bsp1407I 5974 TGTACA T^GTACA
BspHI 957 TCATGA T^CATGA
BsrGI 5974 TGTACA T^GTACA
BssSI 1372 CTCGTG CACGAG(-5/-1)
BstXI 1991 CCATGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5723 CCTGAGG CC^T[ACGT]AGG
DrdI 244 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5723 CCTGAGG CC^T[ACGT]AGG
EcoRI 1336 GAATTC G^AATTC
KasI 273 GGCGCC G^GCGCC
KspI 228 CCGCGG CCGC^GG
NarI 273 GGCGCC GG^CGCC
NcoI 2294 CCATGG C^CATGG
PaeR7I 2331 CTCGAG C^TCGAG
PflFI 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3297 ATTAAT AT^TAAT
PvuI 3689 CGATCG CGAT^CG
RcaI 957 TCATGA T^CATGA
SacI 2 GAGCTC GAGCT^C
SacII 228 CCGCGG CCGC^GG
SexAI 5539 ACCTGGT A^CC[AT]GGT
SfoI 273 GGCGCC GGC^GCC
SspBI 5974 TGTACA T^GTACA
StuI 4149 AGGCCT AGG^CCT
Tth111I 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2331 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3054 GACGTC GACGT^C
5602 GACGTC
AccIII 51 TCCGGA T^CCGGA
85 TCCGGA
AflIII 101 ACGTGT A^C[GA][CT]GT
5703 ACGTGT
Aor51HI 2197 AGCGCT AGC^GCT
4450 AGCGCT
BbrPI 4908 CACGTG CAC^GTG
5702 CACGTG
BciVI 1892 GGATAC GTATCC(6/5)
5562 GTATCC
BlpI 3523 GCTCAGC GC^T[ACGT]AGC
5877 GCTGAGC
Bpu1102I 3523 GCTCAGC GC^T[ACGT]AGC
5877 GCTGAGC
BsaAI 4908 CACGTG [CT]AC^GT[GA]
5702 CACGTG
BsaBI 1893 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4792 GATTTGTATC
BsaWI 51 TCCGGA [AT]^CCGG[AT]
85 TCCGGA
BseAI 51 TCCGGA T^CCGGA
85 TCCGGA
BsmI 622 GAATGC GAATGC(1/-1)
1710 GAATGC
BspEI 51 TCCGGA T^CCGGA
85 TCCGGA
BstAPI 3204 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5582 GCATCACCTGC
BstEII 527 GGTGACC G^GT[ACGT]ACC
5893 GGTGACC
BstPI 527 GGTGACC G^GT[ACGT]ACC
5893 GGTGACC
CelII 3523 GCTCAGC GC^T[ACGT]AGC
5877 GCTGAGC
Eco47III 2197 AGCGCT AGC^GCT
4450 AGCGCT
EcoNI 4274 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5616 CCTGAAAGAGG
EcoO65I 527 GGTGACC G^GT[ACGT]ACC
5893 GGTGACC
HincII 1219 GTTAAC GT[CT]^[GA]AC
5855 GTTAAC
HindII 1219 GTTAAC GT[CT]^[GA]AC
5855 GTTAAC
HpaI 1219 GTTAAC GTT^AAC
5855 GTTAAC
MamI 1893 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4792 GATTTGTATC
MroI 51 TCCGGA T^CCGGA
85 TCCGGA
PmaCI 4908 CACGTG CAC^GTG
5702 CACGTG
PmlI 4908 CACGTG CAC^GTG
5702 CACGTG
SapI 3660 GAAGAGC GCTCTTC(1/4)
1194 GCTCTTC
SfiI 191 GGCCAGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3236 GGCCTGGGTGGCC
SgrAI 275 CGCCGGTG C[GA]^CCGG[CT]G
4727 CACCGGCG
SphI 1469 GCATGC GCATG^C
4974 GCATGC
XbaI 2785 TCTAGA T^CTAGA
2862 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BclI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI XmnI