################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## Acc65I AccI AclI AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AviII AvrII BamHI BlnI BsiWI BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII Eam1105I EcoRV EcoT22I FseI FspI KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I RsrII SalI SbfI ScaI SfuI SnaBI SpeI Sse8387I SwaI XcmI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflII 907 CTTAAG C^TTAAG AocI 5723 CCTGAGG CC^T[ACGT]AGG AseI 3297 ATTAAT AT^TAAT AsnI 3297 ATTAAT AT^TAAT AspI 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 273 GGCGCC GGCGC^C BbvCI 1811 CCTCAGC CCTCAGC(-5/-2) BfrI 907 CTTAAG C^TTAAG Bsp1407I 5974 TGTACA T^GTACA BspHI 957 TCATGA T^CATGA BsrGI 5974 TGTACA T^GTACA BssSI 1372 CTCGTG CACGAG(-5/-1) BstXI 1991 CCATGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 5723 CCTGAGG CC^T[ACGT]AGG DrdI 244 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 5723 CCTGAGG CC^T[ACGT]AGG EcoRI 1336 GAATTC G^AATTC KasI 273 GGCGCC G^GCGCC KspI 228 CCGCGG CCGC^GG NarI 273 GGCGCC GG^CGCC NcoI 2294 CCATGG C^CATGG PaeR7I 2331 CTCGAG C^TCGAG PflFI 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3297 ATTAAT AT^TAAT PvuI 3689 CGATCG CGAT^CG RcaI 957 TCATGA T^CATGA SacI 2 GAGCTC GAGCT^C SacII 228 CCGCGG CCGC^GG SexAI 5539 ACCTGGT A^CC[AT]GGT SfoI 273 GGCGCC GGC^GCC SspBI 5974 TGTACA T^GTACA StuI 4149 AGGCCT AGG^CCT Tth111I 540 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2331 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 3054 GACGTC GACGT^C 5602 GACGTC AccIII 51 TCCGGA T^CCGGA 85 TCCGGA AflIII 101 ACGTGT A^C[GA][CT]GT 5703 ACGTGT Aor51HI 2197 AGCGCT AGC^GCT 4450 AGCGCT BbrPI 4908 CACGTG CAC^GTG 5702 CACGTG BciVI 1892 GGATAC GTATCC(6/5) 5562 GTATCC BlpI 3523 GCTCAGC GC^T[ACGT]AGC 5877 GCTGAGC Bpu1102I 3523 GCTCAGC GC^T[ACGT]AGC 5877 GCTGAGC BsaAI 4908 CACGTG [CT]AC^GT[GA] 5702 CACGTG BsaBI 1893 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4792 GATTTGTATC BsaWI 51 TCCGGA [AT]^CCGG[AT] 85 TCCGGA BseAI 51 TCCGGA T^CCGGA 85 TCCGGA BsmI 622 GAATGC GAATGC(1/-1) 1710 GAATGC BspEI 51 TCCGGA T^CCGGA 85 TCCGGA BstAPI 3204 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5582 GCATCACCTGC BstEII 527 GGTGACC G^GT[ACGT]ACC 5893 GGTGACC BstPI 527 GGTGACC G^GT[ACGT]ACC 5893 GGTGACC CelII 3523 GCTCAGC GC^T[ACGT]AGC 5877 GCTGAGC Eco47III 2197 AGCGCT AGC^GCT 4450 AGCGCT EcoNI 4274 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5616 CCTGAAAGAGG EcoO65I 527 GGTGACC G^GT[ACGT]ACC 5893 GGTGACC HincII 1219 GTTAAC GT[CT]^[GA]AC 5855 GTTAAC HindII 1219 GTTAAC GT[CT]^[GA]AC 5855 GTTAAC HpaI 1219 GTTAAC GTT^AAC 5855 GTTAAC MamI 1893 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4792 GATTTGTATC MroI 51 TCCGGA T^CCGGA 85 TCCGGA PmaCI 4908 CACGTG CAC^GTG 5702 CACGTG PmlI 4908 CACGTG CAC^GTG 5702 CACGTG SapI 3660 GAAGAGC GCTCTTC(1/4) 1194 GCTCTTC SfiI 191 GGCCAGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3236 GGCCTGGGTGGCC SgrAI 275 CGCCGGTG C[GA]^CCGG[CT]G 4727 CACCGGCG SphI 1469 GCATGC GCATG^C 4974 GCATGC XbaI 2785 TCTAGA T^CTAGA 2862 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI