##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII Acc65I AclI AflII AscI
AseI AsnI Asp718I BfrI BsaBI
BsiWI Bst1107I BstBI BstZ17I CpoI
DraI EcoRI EcoRV FseI HpaI
KpnI MamI MluI NdeI NheI
NotI NspV PacI PmeI PshAI
PshBI Psp1406I RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 4456 ACATGT A^C[GA][CT]GT
AgeI 2106 ACCGGT A^CCGGT
AhdI 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4834 AGCGCT AGC^GCT
Asp700I 2130 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1508 TGCGCA TGC^GCA
AvrII 63 CCTAGG C^CTAGG
BbrPI 334 CACGTG CAC^GTG
BciVI 2178 GGATAC GTATCC(6/5)
BclI 1818 TGATCA T^GATCA
BglII 1839 AGATCT A^GATCT
BlnI 63 CCTAGG C^CTAGG
BsmBI 862 CGTCTC CGTCTC(1/5)
BspHI 419 TCATGA T^CATGA
BspLU11I 4456 ACATGT A^CATGT
EagI 13 CGGCCG C^GGCCG
Eam1105I 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 13 CGGCCG C^GGCCG
Eco47III 4834 AGCGCT AGC^GCT
Eco52I 13 CGGCCG C^GGCCG
EcoT22I 5075 ATGCAT ATGCA^T
FbaI 1818 TGATCA T^GATCA
FspI 1508 TGCGCA TGC^GCA
HincII 4566 GTTGAC GT[CT]^[GA]AC
HindII 4566 GTTGAC GT[CT]^[GA]AC
MfeI 6172 CAATTG C^AATTG
MunI 6172 CAATTG C^AATTG
NruI 4375 TCGCGA TCG^CGA
NsiI 5075 ATGCAT ATGCA^T
PaeR7I 1460 CTCGAG C^TCGAG
PinAI 2106 ACCGGT A^CCGGT
PmaCI 334 CACGTG CAC^GTG
PmlI 334 CACGTG CAC^GTG
PvuI 4309 CGATCG CGAT^CG
RcaI 419 TCATGA T^CATGA
SapI 588 GCTCTTC GCTCTTC(1/4)
SbfI 1410 CCTGCAGG CCTGCA^GG
ScaI 2161 AGTACT AGT^ACT
SexAI 842 ACCTGGT A^CC[AT]GGT
Sse8387I 1410 CCTGCAGG CCTGCA^GG
XbaI 6148 TCTAGA T^CTAGA
XhoI 1460 CTCGAG C^TCGAG
XmnI 2130 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2316 TCCGGA T^CCGGA
3325 TCCGGA
Alw44I 331 GTGCAC G^TGCAC
2467 GTGCAC
AocI 1614 CCTGAGG CC^T[ACGT]AGG
3857 CCTGAGG
ApaLI 331 GTGCAC G^TGCAC
2467 GTGCAC
AspI 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACCTTGTC
BalI 1812 TGGCCA TGG^CCA
3629 TGGCCA
BsaAI 334 CACGTG [CT]AC^GT[GA]
4419 TACGTG
BseAI 2316 TCCGGA T^CCGGA
3325 TCCGGA
BsgI 4129 CTGCAC GTGCAG(16/14)
4422 GTGCAG
BsmI 833 GCATTC GAATGC(1/-1)
4573 GCATTC
Bsp1407I 2687 TGTACA T^GTACA
3475 TGTACA
BspDI 2415 ATCGAT AT^CGAT
4311 ATCGAT
BspEI 2316 TCCGGA T^CCGGA
3325 TCCGGA
BsrGI 2687 TGTACA T^GTACA
3475 TGTACA
BssHII 3213 GCGCGC G^CGCGC
4983 GCGCGC
BstAPI 4424 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5068 GCAGATGATGC
BstEII 196 GGTGACC G^GT[ACGT]ACC
368 GGTGACC
BstPI 196 GGTGACC G^GT[ACGT]ACC
368 GGTGACC
Bsu36I 1614 CCTGAGG CC^T[ACGT]AGG
3857 CCTGAGG
ClaI 2415 ATCGAT AT^CGAT
4311 ATCGAT
DrdI 2217 GACGAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2936 GACAGGTAGGTC
Eco81I 1614 CCTGAGG CC^T[ACGT]AGG
3857 CCTGAGG
EcoO65I 196 GGTGACC G^GT[ACGT]ACC
368 GGTGACC
HindIII 75 AAGCTT A^AGCTT
3823 AAGCTT
MluNI 1812 TGGCCA TGG^CCA
3629 TGGCCA
MroI 2316 TCCGGA T^CCGGA
3325 TCCGGA
MscI 1812 TGGCCA TGG^CCA
3629 TGGCCA
NaeI 11 GCCGGC GCC^GGC
1499 GCCGGC
NgoMIV 11 GCCGGC G^CCGGC
1499 GCCGGC
PflFI 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACCTTGTC
SspBI 2687 TGTACA T^GTACA
3475 TGTACA
Tth111I 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI