################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AatII Acc65I AclI AflII AscI AseI AsnI Asp718I BfrI BsaBI BsiWI Bst1107I BstBI BstZ17I CpoI DraI EcoRI EcoRV FseI HpaI KpnI MamI MluI NdeI NheI NotI NspV PacI PmeI PshAI PshBI Psp1406I RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 4456 ACATGT A^C[GA][CT]GT AgeI 2106 ACCGGT A^CCGGT AhdI 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4834 AGCGCT AGC^GCT Asp700I 2130 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1508 TGCGCA TGC^GCA AvrII 63 CCTAGG C^CTAGG BbrPI 334 CACGTG CAC^GTG BciVI 2178 GGATAC GTATCC(6/5) BclI 1818 TGATCA T^GATCA BglII 1839 AGATCT A^GATCT BlnI 63 CCTAGG C^CTAGG BsmBI 862 CGTCTC CGTCTC(1/5) BspHI 419 TCATGA T^CATGA BspLU11I 4456 ACATGT A^CATGT EagI 13 CGGCCG C^GGCCG Eam1105I 1308 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 13 CGGCCG C^GGCCG Eco47III 4834 AGCGCT AGC^GCT Eco52I 13 CGGCCG C^GGCCG EcoT22I 5075 ATGCAT ATGCA^T FbaI 1818 TGATCA T^GATCA FspI 1508 TGCGCA TGC^GCA HincII 4566 GTTGAC GT[CT]^[GA]AC HindII 4566 GTTGAC GT[CT]^[GA]AC MfeI 6172 CAATTG C^AATTG MunI 6172 CAATTG C^AATTG NruI 4375 TCGCGA TCG^CGA NsiI 5075 ATGCAT ATGCA^T PaeR7I 1460 CTCGAG C^TCGAG PinAI 2106 ACCGGT A^CCGGT PmaCI 334 CACGTG CAC^GTG PmlI 334 CACGTG CAC^GTG PvuI 4309 CGATCG CGAT^CG RcaI 419 TCATGA T^CATGA SapI 588 GCTCTTC GCTCTTC(1/4) SbfI 1410 CCTGCAGG CCTGCA^GG ScaI 2161 AGTACT AGT^ACT SexAI 842 ACCTGGT A^CC[AT]GGT Sse8387I 1410 CCTGCAGG CCTGCA^GG XbaI 6148 TCTAGA T^CTAGA XhoI 1460 CTCGAG C^TCGAG XmnI 2130 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 2316 TCCGGA T^CCGGA 3325 TCCGGA Alw44I 331 GTGCAC G^TGCAC 2467 GTGCAC AocI 1614 CCTGAGG CC^T[ACGT]AGG 3857 CCTGAGG ApaLI 331 GTGCAC G^TGCAC 2467 GTGCAC AspI 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4955 GACCTTGTC BalI 1812 TGGCCA TGG^CCA 3629 TGGCCA BsaAI 334 CACGTG [CT]AC^GT[GA] 4419 TACGTG BseAI 2316 TCCGGA T^CCGGA 3325 TCCGGA BsgI 4129 CTGCAC GTGCAG(16/14) 4422 GTGCAG BsmI 833 GCATTC GAATGC(1/-1) 4573 GCATTC Bsp1407I 2687 TGTACA T^GTACA 3475 TGTACA BspDI 2415 ATCGAT AT^CGAT 4311 ATCGAT BspEI 2316 TCCGGA T^CCGGA 3325 TCCGGA BsrGI 2687 TGTACA T^GTACA 3475 TGTACA BssHII 3213 GCGCGC G^CGCGC 4983 GCGCGC BstAPI 4424 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5068 GCAGATGATGC BstEII 196 GGTGACC G^GT[ACGT]ACC 368 GGTGACC BstPI 196 GGTGACC G^GT[ACGT]ACC 368 GGTGACC Bsu36I 1614 CCTGAGG CC^T[ACGT]AGG 3857 CCTGAGG ClaI 2415 ATCGAT AT^CGAT 4311 ATCGAT DrdI 2217 GACGAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2936 GACAGGTAGGTC Eco81I 1614 CCTGAGG CC^T[ACGT]AGG 3857 CCTGAGG EcoO65I 196 GGTGACC G^GT[ACGT]ACC 368 GGTGACC HindIII 75 AAGCTT A^AGCTT 3823 AAGCTT MluNI 1812 TGGCCA TGG^CCA 3629 TGGCCA MroI 2316 TCCGGA T^CCGGA 3325 TCCGGA MscI 1812 TGGCCA TGG^CCA 3629 TGGCCA NaeI 11 GCCGGC GCC^GGC 1499 GCCGGC NgoMIV 11 GCCGGC G^CCGGC 1499 GCCGGC PflFI 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4955 GACCTTGTC SspBI 2687 TGTACA T^GTACA 3475 TGTACA Tth111I 1996 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4955 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI