##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII Alw44I ApaI ApaLI
AseI AsnI Asp700I Asp718I AviII
AvrII BalI BbrPI BbvCI BglI
BlnI BsaI BseAI BspEI CpoI
EcoRI EcoRV FseI FspI HindIII
KpnI KspI MluNI MroI MscI
NaeI NdeI NgoMIV NheI NotI
PacI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SapI SbfI
ScaI SfiI SgrAI SpeI Sse8387I
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 5749 AACGTT AA^CGTT
AflII 419 CTTAAG C^TTAAG
AgeI 2864 ACCGGT A^CCGGT
Aor51HI 5366 AGCGCT AGC^GCT
AscI 5257 GGCGCGCC GG^CGCGCC
BbeI 4829 GGCGCC GGCGC^C
BfrI 419 CTTAAG C^TTAAG
BlpI 5362 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5362 GCTGAGC GC^T[ACGT]AGC
BsaBI 2093 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3774 CGTACG C^GTACG
BsmI 5244 GCATTC GAATGC(1/-1)
Bsp1407I 1842 TGTACA T^GTACA
BspLU11I 3039 ACATGT A^CATGT
BsrGI 1842 TGTACA T^GTACA
Bst1107I 747 GTATAC GTA^TAC
BstZ17I 747 GTATAC GTA^TAC
CelII 5362 GCTGAGC GC^T[ACGT]AGC
DraI 5664 TTTAAA TTT^AAA
Eco47III 5366 AGCGCT AGC^GCT
EcoT22I 1391 ATGCAT ATGCA^T
HpaI 4604 GTTAAC GTT^AAC
KasI 4829 GGCGCC G^GCGCC
MamI 2093 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5701 CAATTG C^AATTG
MluI 3771 ACGCGT A^CGCGT
MunI 5701 CAATTG C^AATTG
NarI 4829 GGCGCC GG^CGCC
NruI 1431 TCGCGA TCG^CGA
NsiI 1391 ATGCAT ATGCA^T
PaeR7I 4029 CTCGAG C^TCGAG
PinAI 2864 ACCGGT A^CCGGT
Psp1406I 5749 AACGTT AA^CGTT
PstI 5571 CTGCAG CTGCA^G
SacI 1946 GAGCTC GAGCT^C
SalI 4376 GTCGAC G^TCGAC
SexAI 2133 ACCAGGT A^CC[AT]GGT
SfoI 4829 GGCGCC GGC^GCC
SspBI 1842 TGTACA T^GTACA
StuI 4773 AGGCCT AGG^CCT
XhoI 4029 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2042 GACGTC GACGT^C
3785 GACGTC
AocI 302 CCTGAGG CC^T[ACGT]AGG
2776 CCTGAGG
AspI 1052 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5119 GACCGTGTC
BamHI 1869 GGATCC G^GATCC
2092 GGATCC
BcgI 2097 CGAATCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5317 CGAGGAGGGTGC
BfaI 1386 CTAG C^TAG
4098 CTAG
BsmBI 1075 CGTCTC CGTCTC(1/5)
1773 GAGACG
BspDI 1259 ATCGAT AT^CGAT
2429 ATCGAT
BssSI 2896 CACGAG CACGAG(-5/-1)
4156 CACGAG
BstAPI 837 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5299 GCAGCGCGTGC
BstEII 3721 GGTAACC G^GT[ACGT]ACC
5484 GGTAACC
BstPI 3721 GGTAACC G^GT[ACGT]ACC
5484 GGTAACC
Bsu36I 302 CCTGAGG CC^T[ACGT]AGG
2776 CCTGAGG
ClaI 1259 ATCGAT AT^CGAT
2429 ATCGAT
DraIII 146 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
148 CACTGTGTG
Eco81I 302 CCTGAGG CC^T[ACGT]AGG
2776 CCTGAGG
EcoNI 285 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
457 CCTCATGAAGG
EcoO65I 3721 GGTAACC G^GT[ACGT]ACC
5484 GGTAACC
MaeI 1386 CTAG C^TAG
4098 CTAG
PflFI 1052 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5119 GACCGTGTC
SmaI 5277 CCCGGG CCC^GGG
5491 CCCGGG
SmlI 419 CTTAAG C^T[CT][GA]AG
4029 CTCGAG
SnaBI 926 TACGTA TAC^GTA
5399 TACGTA
SphI 1389 GCATGC GCATG^C
3474 GCATGC
Tth111I 1052 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5119 GACCGTGTC
XmaCI 5277 CCCGGG C^CCGGG
5491 CCCGGG
XmaI 5277 CCCGGG C^CCGGG
5491 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI ApoI AspEI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BclI BcnI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstBI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
NspV PflMI PleI PpuMI PshAI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII