##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AvrII BcgI
BfrI BlnI BsaBI BsiWI BsmBI
BspDI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoNI
FseI HindIII HpaI MamI MfeI
MunI NaeI NgoMIV NheI NspV
PacI PinAI PmeI PvuI RsrII
ScaI SexAI SfiI SfuI SnaBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1639 GACGTC GACGT^C
Acc65I 2842 GGTACC G^GTACC
AccIII 3190 TCCGGA T^CCGGA
AclI 798 AACGTT AA^CGTT
ApaI 4327 GGGCCC GGGCC^C
Asp718I 2842 GGTACC G^GTACC
AviII 1433 TGCGCA TGC^GCA
BamHI 1631 GGATCC G^GATCC
BbvCI 3298 GCTGAGG CCTCAGC(-5/-2)
BglI 775 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4272 AGATCT A^GATCT
BlpI 1353 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1353 GCTCAGC GC^T[ACGT]AGC
BseAI 3190 TCCGGA T^CCGGA
Bsp1407I 5438 TGTACA T^GTACA
BspEI 3190 TCCGGA T^CCGGA
BspHI 3260 TCATGA T^CATGA
BsrBI 5405 CCGCTC CCGCTC(-3/-3)
BsrGI 5438 TGTACA T^GTACA
BssHII 1569 GCGCGC G^CGCGC
BstEII 2363 GGTCACC G^GT[ACGT]ACC
BstPI 2363 GGTCACC G^GT[ACGT]ACC
CelII 1353 GCTCAGC GC^T[ACGT]AGC
EagI 1341 CGGCCG C^GGCCG
EclXI 1341 CGGCCG C^GGCCG
Eco52I 1341 CGGCCG C^GGCCG
EcoO65I 2363 GGTCACC G^GT[ACGT]ACC
EcoRV 5606 GATATC GAT^ATC
FspI 1433 TGCGCA TGC^GCA
KpnI 2842 GGTACC GGTAC^C
MluI 1223 ACGCGT A^CGCGT
MroI 3190 TCCGGA T^CCGGA
NcoI 2643 CCATGG C^CATGG
NdeI 3335 CATATG CA^TATG
NotI 1340 GCGGCCGC GC^GGCCGC
NruI 2440 TCGCGA TCG^CGA
PflMI 539 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 857 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 798 AACGTT AA^CGTT
RcaI 3260 TCATGA T^CATGA
SapI 1539 GCTCTTC GCTCTTC(1/4)
SbfI 4795 CCTGCAGG CCTGCA^GG
SgrAI 662 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1182 CCCGGG CCC^GGG
SpeI 2142 ACTAGT A^CTAGT
Sse8387I 4795 CCTGCAGG CCTGCA^GG
SspBI 5438 TGTACA T^GTACA
Van91I 539 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1182 CCCGGG C^CCGGG
XmaI 1182 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1792 GTCGAC GT^[AC][GT]AC
5094 GTCGAC
AocI 135 CCTCAGG CC^T[ACGT]AGG
4595 CCTCAGG
AseI 2113 ATTAAT AT^TAAT
4702 ATTAAT
AsnI 2113 ATTAAT AT^TAAT
4702 ATTAAT
Asp700I 490 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4222 GAAGGCTTTC
AspI 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4853 GACTTGGTC
BbeI 1186 GGCGCC GGCGC^C
1417 GGCGCC
BbrPI 2812 CACGTG CAC^GTG
5473 CACGTG
BciVI 815 GTATCC GTATCC(6/5)
3341 GTATCC
BclI 3561 TGATCA T^GATCA
3594 TGATCA
BsaWI 3190 TCCGGA [AT]^CCGG[AT]
3276 TCCGGT
BsgI 4621 CTGCAC GTGCAG(16/14)
3153 GTGCAG
BsiEI 1341 CGGCCG CG[GA][CT]^CG
1473 CGACCG
BsmI 2571 GAATGC GAATGC(1/-1)
5716 GAATGC
BssSI 4523 CACGAG CACGAG(-5/-1)
1279 CTCGTG
Bsu36I 135 CCTCAGG CC^T[ACGT]AGG
4595 CCTCAGG
Eco81I 135 CCTCAGG CC^T[ACGT]AGG
4595 CCTCAGG
EcoRI 2261 GAATTC G^AATTC
4841 GAATTC
FbaI 3561 TGATCA T^GATCA
3594 TGATCA
KasI 1186 GGCGCC G^GCGCC
1417 GGCGCC
KspI 1272 CCGCGG CCGC^GG
1574 CCGCGG
NarI 1186 GGCGCC GG^CGCC
1417 GGCGCC
PflFI 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4853 GACTTGGTC
PmaCI 2812 CACGTG CAC^GTG
5473 CACGTG
PmlI 2812 CACGTG CAC^GTG
5473 CACGTG
PshBI 2113 ATTAAT AT^TAAT
4702 ATTAAT
SacII 1272 CCGCGG CCGC^GG
1574 CCGCGG
SalI 1792 GTCGAC G^TCGAC
5094 GTCGAC
SfoI 1186 GGCGCC GGC^GCC
1417 GGCGCC
SphI 160 GCATGC GCATG^C
420 GCATGC
Tth111I 1038 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4853 GACTTGGTC
XmnI 490 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4222 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoI XhoII