##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII Acc65I AclI Alw44I ApaI
ApaLI AscI Asp718I AviII BbeI
BbvCI BsaBI BsiWI BssHII CpoI
DrdI EcoT22I FseI FspI KasI
KpnI MamI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PmeI Psp1406I PvuI
RsrII SalI SbfI SfoI Sse8387I
SwaI XcmI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 5308 CGCG CG^CG
AccIII 3300 TCCGGA T^CCGGA
AcyI 4170 GGCGTC G[GA]^CG[CT]C
AflII 1483 CTTAAG C^TTAAG
AgeI 4153 ACCGGT A^CCGGT
AocI 6346 CCTAAGG CC^T[ACGT]AGG
AseI 3721 ATTAAT AT^TAAT
AsnI 3721 ATTAAT AT^TAAT
AspI 1510 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3055 GGATCC G^GATCC
BbrPI 4942 CACGTG CAC^GTG
BcgI 2185 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1483 CTTAAG C^TTAAG
BsaHI 4170 GGCGTC G[GA]^CG[CT]C
BsaI 4237 GGTCTC GGTCTC(1/5)
BseAI 3300 TCCGGA T^CCGGA
BsiEI 5251 CGGCCG CG[GA][CT]^CG
BsmI 4680 GCATTC GAATGC(1/-1)
Bsp1407I 2326 TGTACA T^GTACA
BspDI 4606 ATCGAT AT^CGAT
BspEI 3300 TCCGGA T^CCGGA
BspLU11I 4021 ACATGT A^CATGT
BsrFI 4153 ACCGGT [GA]^CCGG[CT]
BsrGI 2326 TGTACA T^GTACA
BstBI 5118 TTCGAA TT^CGAA
BstUI 5308 CGCG CG^CG
BstXI 2990 CCATTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 6346 CCTAAGG CC^T[ACGT]AGG
Cfr10I 4153 ACCGGT [GA]^CCGG[CT]
ClaI 4606 ATCGAT AT^CGAT
DraIII 6000 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5251 CGGCCG C^GGCCG
EclXI 5251 CGGCCG C^GGCCG
Eco52I 5251 CGGCCG C^GGCCG
Eco81I 6346 CCTAAGG CC^T[ACGT]AGG
Hin1I 4170 GGCGTC G[GA]^CG[CT]C
HpaI 1140 GTTAAC GTT^AAC
KspI 5307 CCGCGG CCGC^GG
MroI 3300 TCCGGA T^CCGGA
MvnI 5308 CGCG CG^CG
NspV 5118 TTCGAA TT^CGAA
PflFI 1510 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 592 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4153 ACCGGT A^CCGGT
PmaCI 4942 CACGTG CAC^GTG
PmlI 4942 CACGTG CAC^GTG
PshAI 4020 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3721 ATTAAT AT^TAAT
SacII 5307 CCGCGG CCGC^GG
SexAI 3652 ACCAGGT A^CC[AT]GGT
SfiI 5305 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5118 TTCGAA TT^CGAA
SgrAI 4152 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 5605 TACGTA TAC^GTA
SpeI 5868 ACTAGT A^CTAGT
SspBI 2326 TGTACA T^GTACA
Tth111I 1510 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 592 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 2787 ACGTGT A^C[GA][CT]GT
4021 ACATGT
AhdI 577 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4272 GACGATGAGTC
Aor51HI 43 AGCGCT AGC^GCT
4694 AGCGCT
AspEI 577 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4272 GACGATGAGTC
AvrII 2493 CCTAGG C^CTAGG
5786 CCTAGG
BglII 887 AGATCT A^GATCT
3758 AGATCT
BlnI 2493 CCTAGG C^CTAGG
5786 CCTAGG
BlpI 46 GCTGAGC GC^T[ACGT]AGC
1637 GCTCAGC
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
1637 GCTCAGC
BsaWI 3300 TCCGGA [AT]^CCGG[AT]
4153 ACCGGT
BsmBI 4172 CGTCTC CGTCTC(1/5)
4226 GAGACG
BsrBI 5883 CCGCTC CCGCTC(-3/-3)
1978 GAGCGG
BsrDI 3867 CATTGC GCAATG(2/0)
6140 CATTGC
BssSI 4920 CACGAG CACGAG(-5/-1)
5042 CTCGTG
Bst1107I 960 GTATAC GTA^TAC
3636 GTATAC
BstZ17I 960 GTATAC GTA^TAC
3636 GTATAC
CelII 46 GCTGAGC GC^T[ACGT]AGC
1637 GCTCAGC
DsaI 523 CCACGG C^C[GA][CT]GG
5307 CCGCGG
Eam1105I 577 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4272 GACGATGAGTC
Eco47III 43 AGCGCT AGC^GCT
4694 AGCGCT
EcoRI 663 GAATTC G^AATTC
1110 GAATTC
EcoRV 3172 GATATC GAT^ATC
3181 GATATC
HgaI 2934 GACGC GACGC(5/10)
4171 GCGTC
HincII 1140 GTTAAC GT[CT]^[GA]AC
4633 GTTGAC
HindII 1140 GTTAAC GT[CT]^[GA]AC
4633 GTTGAC
MfeI 1243 CAATTG C^AATTG
3532 CAATTG
MunI 1243 CAATTG C^AATTG
3532 CAATTG
PpuMI 3897 AGGTCCT [GA]G^G[AT]CC[CT]
5232 GGGTCCT
SapI 3559 GCTCTTC GCTCTTC(1/4)
3664 GCTCTTC
SmaI 120 CCCGGG CCC^GGG
5285 CCCGGG
SspI 2737 AATATT AAT^ATT
5535 AATATT
StuI 2223 AGGCCT AGG^CCT
5890 AGGCCT
XbaI 1197 TCTAGA T^CTAGA
5299 TCTAGA
XmaCI 120 CCCGGG C^CCGGG
5285 CCCGGG
XmaI 120 CCCGGG C^CCGGG
5285 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PaeR7I PleI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoI XhoII XmnI