##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AviII AvrII BlnI BsaBI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI FseI FspI KpnI KspI
MamI MfeI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2190 GACGTC GACGT^C
AccI 4263 GTCTAC GT^[AC][GT]AC
AccIII 1921 TCCGGA T^CCGGA
AcsI 6100 GAATTT [GA]^AATT[CT]
AflII 6074 CTTAAG C^TTAAG
AhdI 4976 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3122 AGCGCT AGC^GCT
ApoI 6100 GAATTT [GA]^AATT[CT]
AspEI 4976 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1692 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1236 GGATCC G^GATCC
BbrPI 4068 CACGTG CAC^GTG
BcgI 3539 CGACTTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2103 GGATAC GTATCC(6/5)
BfrI 6074 CTTAAG C^TTAAG
BsaWI 1921 TCCGGA [AT]^CCGG[AT]
BseAI 1921 TCCGGA T^CCGGA
BspEI 1921 TCCGGA T^CCGGA
BspLU11I 3931 ACATGT A^CATGT
BsrBI 1470 GAGCGG CCGCTC(-3/-3)
DraI 5804 TTTAAA TTT^AAA
Eam1105I 4976 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3122 AGCGCT AGC^GCT
EcoNI 6031 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3947 GATATC GAT^ATC
EcoT22I 3919 ATGCAT ATGCA^T
HpaI 5344 GTTAAC GTT^AAC
MluI 1076 ACGCGT A^CGCGT
MroI 1921 TCCGGA T^CCGGA
NaeI 3386 GCCGGC GCC^GGC
NgoMIV 3386 GCCGGC G^CCGGC
NsiI 3919 ATGCAT ATGCA^T
PflFI 1692 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4068 CACGTG CAC^GTG
PmlI 4068 CACGTG CAC^GTG
SbfI 3499 CCTGCAGG CCTGCA^GG
Sse8387I 3499 CCTGCAGG CCTGCA^GG
StuI 649 AGGCCT AGG^CCT
Tth111I 1692 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1992 GTGCAC G^TGCAC
2982 GTGCAC
ApaLI 1992 GTGCAC G^TGCAC
2982 GTGCAC
AseI 5551 ATTAAT AT^TAAT
6280 ATTAAT
AsnI 5551 ATTAAT AT^TAAT
6280 ATTAAT
BbeI 1138 GGCGCC GGCGC^C
2336 GGCGCC
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
3519 GCTGAGG
BclI 4185 TGATCA T^GATCA
6254 TGATCA
BsaAI 1626 TACGTG [CT]AC^GT[GA]
4068 CACGTG
BsrFI 2659 ACCGGC [GA]^CCGG[CT]
3386 GCCGGC
BssHII 1023 GCGCGC G^CGCGC
1175 GCGCGC
BssSI 2292 CACGAG CACGAG(-5/-1)
3186 CTCGTG
Cfr10I 2659 ACCGGC [GA]^CCGG[CT]
3386 GCCGGC
DraIII 1984 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2601 CACGGAGTG
DrdI 4725 GACCCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5579 GACAGGATTGTC
FbaI 4185 TGATCA T^GATCA
6254 TGATCA
HindIII 387 AAGCTT A^AGCTT
2182 AAGCTT
KasI 1138 GGCGCC G^GCGCC
2336 GGCGCC
NarI 1138 GGCGCC GG^CGCC
2336 GGCGCC
PshAI 65 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1418 GACTCGGGTC
PshBI 5551 ATTAAT AT^TAAT
6280 ATTAAT
SacI 1103 GAGCTC GAGCT^C
3136 GAGCTC
SexAI 511 ACCAGGT A^CC[AT]GGT
1985 ACCTGGT
SfoI 1138 GGCGCC GGC^GCC
2336 GGCGCC
XcmI 1666 CCAGCCCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2240 CCAGTTAGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI