##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BbeI
BglI BglII BlnI BsiWI BsmBI
Bsp1407I BspDI BsrBI BsrGI BssHII
BstUI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HindIII HpaI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PflMI PinAI
PmeI PshBI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SspBI SspI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 3072 TCCGGA T^CCGGA
AclI 1450 AACGTT AA^CGTT
AcyI 1378 GACGCC G[GA]^CG[CT]C
AflII 1061 CTTAAG C^TTAAG
BamHI 3682 GGATCC G^GATCC
BbrPI 2401 CACGTG CAC^GTG
BclI 3519 TGATCA T^GATCA
BfrI 1061 CTTAAG C^TTAAG
BsaBI 3749 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1378 GACGCC G[GA]^CG[CT]C
BseAI 3072 TCCGGA T^CCGGA
BsiEI 997 CGGTCG CG[GA][CT]^CG
BspEI 3072 TCCGGA T^CCGGA
BspHI 1483 TCATGA T^CATGA
BspLU11I 1345 ACATGT A^CATGT
BsrFI 1802 ACCGGC [GA]^CCGG[CT]
BssSI 3617 CACGAG CACGAG(-5/-1)
Bst1107I 3736 GTATAC GTA^TAC
BstAPI 3273 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2937 TTCGAA TT^CGAA
BstEII 2839 GGTGACC G^GT[ACGT]ACC
BstPI 2839 GGTGACC G^GT[ACGT]ACC
BstZ17I 3736 GTATAC GTA^TAC
Cfr10I 1802 ACCGGC [GA]^CCGG[CT]
DrdI 1718 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2161 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2839 GGTGACC G^GT[ACGT]ACC
EcoRI 3810 GAATTC G^AATTC
FbaI 3519 TGATCA T^GATCA
HgaI 1378 GACGC GACGC(5/10)
Hin1I 1378 GACGCC G[GA]^CG[CT]C
MamI 3749 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3072 TCCGGA T^CCGGA
NcoI 1626 CCATGG C^CATGG
NdeI 83 CATATG CA^TATG
NspV 2937 TTCGAA TT^CGAA
PaeR7I 2607 CTCGAG C^TCGAG
PmaCI 2401 CACGTG CAC^GTG
PmlI 2401 CACGTG CAC^GTG
PshAI 1310 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1450 AACGTT AA^CGTT
RcaI 1483 TCATGA T^CATGA
SacI 2966 GAGCTC GAGCT^C
ScaI 2181 AGTACT AGT^ACT
SfuI 2937 TTCGAA TT^CGAA
SmaI 1787 CCCGGG CCC^GGG
SpeI 3981 ACTAGT A^CTAGT
TfiI 2107 GATTC G^A[AT]TC
XcmI 3495 CCAATTCCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2607 CTCGAG C^TCGAG
XmaCI 1787 CCCGGG C^CCGGG
XmaI 1787 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3127 GGTACC G^GTACC
3225 GGTACC
Asp700I 1895 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3033 GAAGCCTTTC
Asp718I 3127 GGTACC G^GTACC
3225 GGTACC
AspHI 2966 GAGCTC G[AT]GC[AT]^C
3957 GTGCTC
AspI 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2066 GACCGAGTC
AvaI 1787 CCCGGG C^[CT]CG[GA]G
2607 CTCGAG
BanII 2765 GAGCCC G[GA]GC[CT]^C
2966 GAGCTC
BcgI 2609 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2599 GCAGAGGCCTCG
BciVI 3990 GGATAC GTATCC(6/5)
2827 GTATCC
BlpI 1432 GCTCAGC GC^T[ACGT]AGC
2692 GCTGAGC
Bpu1102I 1432 GCTCAGC GC^T[ACGT]AGC
2692 GCTGAGC
BsaI 1295 GAGACC GGTCTC(1/5)
4082 GGTCTC
BsaWI 3072 TCCGGA [AT]^CCGG[AT]
3124 TCCGGT
BsgI 1924 CTGCAC GTGCAG(16/14)
4069 CTGCAC
BsiHKAI 2966 GAGCTC G[AT]GC[AT]^C
3957 GTGCTC
BsmI 2311 GAATGC GAATGC(1/-1)
2934 GCATTC
BsoBI 1787 CCCGGG C^[CT]CG[GA]G
2607 CTCGAG
BspMI 475 ACCTGC ACCTGC(4/8)
3973 ACCTGC
BstYI 1655 AGATCC [GA]^GATC[CT]
3682 GGATCC
CelII 1432 GCTCAGC GC^T[ACGT]AGC
2692 GCTGAGC
DraII 1866 GGGTCCC [GA]G^G[ACGT]CC[CT]
2526 AGGACCT
DsaI 217 CCACGG C^C[GA][CT]GG
1626 CCATGG
EcoO109I 1866 GGGTCCC [GA]G^G[ACGT]CC[CT]
2526 AGGACCT
EcoT22I 1889 ATGCAT ATGCA^T
3168 ATGCAT
HincII 48 GTCAAC GT[CT]^[GA]AC
3406 GTTGAC
HindII 48 GTCAAC GT[CT]^[GA]AC
3406 GTTGAC
KpnI 3127 GGTACC GGTAC^C
3225 GGTACC
MflI 1655 AGATCC [GA]^GATC[CT]
3682 GGATCC
NsiI 1889 ATGCAT ATGCA^T
3168 ATGCAT
PflFI 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2066 GACCGAGTC
PpuMI 1866 GGGTCCC [GA]G^G[AT]CC[CT]
2526 AGGACCT
PstI 1995 CTGCAG CTGCA^G
3975 CTGCAG
StuI 2058 AGGCCT AGG^CCT
2603 AGGCCT
Tth111I 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2066 GACCGAGTC
XhoII 1655 AGATCC [GA]^GATC[CT]
3682 GGATCC
XmnI 1895 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3033 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AvaII BalI BanI BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI Eco81I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI