################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BglI BglII BlnI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrGI BssHII BstUI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HindIII HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PflMI PinAI PmeI PshBI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI Van91I ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 3072 TCCGGA T^CCGGA AclI 1450 AACGTT AA^CGTT AcyI 1378 GACGCC G[GA]^CG[CT]C AflII 1061 CTTAAG C^TTAAG BamHI 3682 GGATCC G^GATCC BbrPI 2401 CACGTG CAC^GTG BclI 3519 TGATCA T^GATCA BfrI 1061 CTTAAG C^TTAAG BsaBI 3749 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1378 GACGCC G[GA]^CG[CT]C BseAI 3072 TCCGGA T^CCGGA BsiEI 997 CGGTCG CG[GA][CT]^CG BspEI 3072 TCCGGA T^CCGGA BspHI 1483 TCATGA T^CATGA BspLU11I 1345 ACATGT A^CATGT BsrFI 1802 ACCGGC [GA]^CCGG[CT] BssSI 3617 CACGAG CACGAG(-5/-1) Bst1107I 3736 GTATAC GTA^TAC BstAPI 3273 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2937 TTCGAA TT^CGAA BstEII 2839 GGTGACC G^GT[ACGT]ACC BstPI 2839 GGTGACC G^GT[ACGT]ACC BstZ17I 3736 GTATAC GTA^TAC Cfr10I 1802 ACCGGC [GA]^CCGG[CT] DrdI 1718 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2161 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2839 GGTGACC G^GT[ACGT]ACC EcoRI 3810 GAATTC G^AATTC FbaI 3519 TGATCA T^GATCA HgaI 1378 GACGC GACGC(5/10) Hin1I 1378 GACGCC G[GA]^CG[CT]C MamI 3749 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3072 TCCGGA T^CCGGA NcoI 1626 CCATGG C^CATGG NdeI 83 CATATG CA^TATG NspV 2937 TTCGAA TT^CGAA PaeR7I 2607 CTCGAG C^TCGAG PmaCI 2401 CACGTG CAC^GTG PmlI 2401 CACGTG CAC^GTG PshAI 1310 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1450 AACGTT AA^CGTT RcaI 1483 TCATGA T^CATGA SacI 2966 GAGCTC GAGCT^C ScaI 2181 AGTACT AGT^ACT SfuI 2937 TTCGAA TT^CGAA SmaI 1787 CCCGGG CCC^GGG SpeI 3981 ACTAGT A^CTAGT TfiI 2107 GATTC G^A[AT]TC XcmI 3495 CCAATTCCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2607 CTCGAG C^TCGAG XmaCI 1787 CCCGGG C^CCGGG XmaI 1787 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 3127 GGTACC G^GTACC 3225 GGTACC Asp700I 1895 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3033 GAAGCCTTTC Asp718I 3127 GGTACC G^GTACC 3225 GGTACC AspHI 2966 GAGCTC G[AT]GC[AT]^C 3957 GTGCTC AspI 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2066 GACCGAGTC AvaI 1787 CCCGGG C^[CT]CG[GA]G 2607 CTCGAG BanII 2765 GAGCCC G[GA]GC[CT]^C 2966 GAGCTC BcgI 2609 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2599 GCAGAGGCCTCG BciVI 3990 GGATAC GTATCC(6/5) 2827 GTATCC BlpI 1432 GCTCAGC GC^T[ACGT]AGC 2692 GCTGAGC Bpu1102I 1432 GCTCAGC GC^T[ACGT]AGC 2692 GCTGAGC BsaI 1295 GAGACC GGTCTC(1/5) 4082 GGTCTC BsaWI 3072 TCCGGA [AT]^CCGG[AT] 3124 TCCGGT BsgI 1924 CTGCAC GTGCAG(16/14) 4069 CTGCAC BsiHKAI 2966 GAGCTC G[AT]GC[AT]^C 3957 GTGCTC BsmI 2311 GAATGC GAATGC(1/-1) 2934 GCATTC BsoBI 1787 CCCGGG C^[CT]CG[GA]G 2607 CTCGAG BspMI 475 ACCTGC ACCTGC(4/8) 3973 ACCTGC BstYI 1655 AGATCC [GA]^GATC[CT] 3682 GGATCC CelII 1432 GCTCAGC GC^T[ACGT]AGC 2692 GCTGAGC DraII 1866 GGGTCCC [GA]G^G[ACGT]CC[CT] 2526 AGGACCT DsaI 217 CCACGG C^C[GA][CT]GG 1626 CCATGG EcoO109I 1866 GGGTCCC [GA]G^G[ACGT]CC[CT] 2526 AGGACCT EcoT22I 1889 ATGCAT ATGCA^T 3168 ATGCAT HincII 48 GTCAAC GT[CT]^[GA]AC 3406 GTTGAC HindII 48 GTCAAC GT[CT]^[GA]AC 3406 GTTGAC KpnI 3127 GGTACC GGTAC^C 3225 GGTACC MflI 1655 AGATCC [GA]^GATC[CT] 3682 GGATCC NsiI 1889 ATGCAT ATGCA^T 3168 ATGCAT PflFI 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2066 GACCGAGTC PpuMI 1866 GGGTCCC [GA]G^G[AT]CC[CT] 2526 AGGACCT PstI 1995 CTGCAG CTGCA^G 3975 CTGCAG StuI 2058 AGGCCT AGG^CCT 2603 AGGCCT Tth111I 928 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2066 GACCGAGTC XhoII 1655 AGATCC [GA]^GATC[CT] 3682 GGATCC XmnI 1895 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3033 GAAGCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AvaII BalI BanI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI