##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AgeI Alw44I ApaLI AscI AseI
AsnI BclI BsaBI BsiWI BspDI
BspLU11I BssHII BstAPI BstBI ClaI
CpoI DraI EcoRI EcoT22I FbaI
FseI HpaI KspI MamI MluI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SfuI
SgrAI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 4076 TCCGGA T^CCGGA
AclI 6100 AACGTT AA^CGTT
AflII 7079 CTTAAG C^TTAAG
Aor51HI 5385 AGCGCT AGC^GCT
AvrII 6932 CCTAGG C^CTAGG
BbrPI 6203 CACGTG CAC^GTG
BcgI 1761 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 7079 CTTAAG C^TTAAG
BlnI 6932 CCTAGG C^CTAGG
BseAI 4076 TCCGGA T^CCGGA
BspEI 4076 TCCGGA T^CCGGA
BspHI 1586 TCATGA T^CATGA
Bst1107I 1164 GTATAC GTA^TAC
BstZ17I 1164 GTATAC GTA^TAC
Eco47III 5385 AGCGCT AGC^GCT
MroI 4076 TCCGGA T^CCGGA
NdeI 1740 CATATG CA^TATG
NheI 7024 GCTAGC G^CTAGC
PmaCI 6203 CACGTG CAC^GTG
PmlI 6203 CACGTG CAC^GTG
Psp1406I 6100 AACGTT AA^CGTT
RcaI 1586 TCATGA T^CATGA
SapI 6474 GCTCTTC GCTCTTC(1/4)
SnaBI 5035 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1875 AAATTC [GA]^AATT[CT]
2053 AAATTC
AhdI 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
916 GACGAGGCGTC
ApoI 1875 AAATTC [GA]^AATT[CT]
2053 AAATTC
AspEI 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
916 GACGAGGCGTC
AspI 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5797 GACCACGTC
BciVI 4438 GGATAC GTATCC(6/5)
6198 GGATAC
BglII 5615 AGATCT A^GATCT
6395 AGATCT
BsmI 1902 GAATGC GAATGC(1/-1)
6750 GAATGC
Bsp1407I 5495 TGTACA T^GTACA
7011 TGTACA
BsrGI 5495 TGTACA T^GTACA
7011 TGTACA
BssSI 2137 CACGAG CACGAG(-5/-1)
3157 CTCGTG
Eam1105I 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
916 GACGAGGCGTC
EcoRV 5089 GATATC GAT^ATC
7087 GATATC
HindIII 5409 AAGCTT A^AGCTT
6351 AAGCTT
MfeI 7589 CAATTG C^AATTG
8086 CAATTG
MunI 7589 CAATTG C^AATTG
8086 CAATTG
NaeI 301 GCCGGC GCC^GGC
874 GCCGGC
NgoMIV 301 GCCGGC G^CCGGC
874 GCCGGC
PflFI 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5797 GACCACGTC
SacI 4162 GAGCTC GAGCT^C
5440 GAGCTC
SbfI 3954 CCTGCAGG CCTGCA^GG
6833 CCTGCAGG
ScaI 2063 AGTACT AGT^ACT
5342 AGTACT
SfiI 1071 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
7731 GGCCTTCCAGGCC
Sse8387I 3954 CCTGCAGG CCTGCA^GG
6833 CCTGCAGG
SspBI 5495 TGTACA T^GTACA
7011 TGTACA
Tth111I 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5797 GACCACGTC
XbaI 2722 TCTAGA T^CTAGA
6356 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI Asp700I Asp718I
AspHI AvaI AvaII AviII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI