################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AgeI Alw44I ApaLI AscI AseI AsnI BclI BsaBI BsiWI BspDI BspLU11I BssHII BstAPI BstBI ClaI CpoI DraI EcoRI EcoT22I FbaI FseI HpaI KspI MamI MluI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SfuI SgrAI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AccIII 4076 TCCGGA T^CCGGA AclI 6100 AACGTT AA^CGTT AflII 7079 CTTAAG C^TTAAG Aor51HI 5385 AGCGCT AGC^GCT AvrII 6932 CCTAGG C^CTAGG BbrPI 6203 CACGTG CAC^GTG BcgI 1761 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 7079 CTTAAG C^TTAAG BlnI 6932 CCTAGG C^CTAGG BseAI 4076 TCCGGA T^CCGGA BspEI 4076 TCCGGA T^CCGGA BspHI 1586 TCATGA T^CATGA Bst1107I 1164 GTATAC GTA^TAC BstZ17I 1164 GTATAC GTA^TAC Eco47III 5385 AGCGCT AGC^GCT MroI 4076 TCCGGA T^CCGGA NdeI 1740 CATATG CA^TATG NheI 7024 GCTAGC G^CTAGC PmaCI 6203 CACGTG CAC^GTG PmlI 6203 CACGTG CAC^GTG Psp1406I 6100 AACGTT AA^CGTT RcaI 1586 TCATGA T^CATGA SapI 6474 GCTCTTC GCTCTTC(1/4) SnaBI 5035 TACGTA TAC^GTA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 1875 AAATTC [GA]^AATT[CT] 2053 AAATTC AhdI 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 916 GACGAGGCGTC ApoI 1875 AAATTC [GA]^AATT[CT] 2053 AAATTC AspEI 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 916 GACGAGGCGTC AspI 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5797 GACCACGTC BciVI 4438 GGATAC GTATCC(6/5) 6198 GGATAC BglII 5615 AGATCT A^GATCT 6395 AGATCT BsmI 1902 GAATGC GAATGC(1/-1) 6750 GAATGC Bsp1407I 5495 TGTACA T^GTACA 7011 TGTACA BsrGI 5495 TGTACA T^GTACA 7011 TGTACA BssSI 2137 CACGAG CACGAG(-5/-1) 3157 CTCGTG Eam1105I 709 GACGGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 916 GACGAGGCGTC EcoRV 5089 GATATC GAT^ATC 7087 GATATC HindIII 5409 AAGCTT A^AGCTT 6351 AAGCTT MfeI 7589 CAATTG C^AATTG 8086 CAATTG MunI 7589 CAATTG C^AATTG 8086 CAATTG NaeI 301 GCCGGC GCC^GGC 874 GCCGGC NgoMIV 301 GCCGGC G^CCGGC 874 GCCGGC PflFI 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5797 GACCACGTC SacI 4162 GAGCTC GAGCT^C 5440 GAGCTC SbfI 3954 CCTGCAGG CCTGCA^GG 6833 CCTGCAGG ScaI 2063 AGTACT AGT^ACT 5342 AGTACT SfiI 1071 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 7731 GGCCTTCCAGGCC Sse8387I 3954 CCTGCAGG CCTGCA^GG 6833 CCTGCAGG SspBI 5495 TGTACA T^GTACA 7011 TGTACA Tth111I 1140 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5797 GACCACGTC XbaI 2722 TCTAGA T^CTAGA 6356 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 162 enzymes) # ######################################### AatII Acc65I AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI Asp700I Asp718I AspHI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI