##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BpmI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrDI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI FseI FspI HaeII
Hin1I KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1753 GGTACC G^GTACC
AclI 50 AACGTT AA^CGTT
AflIII 396 ACATGT A^C[GA][CT]GT
ApaI 1676 GGGCCC GGGCC^C
AseI 2440 ATTAAT AT^TAAT
AsnI 2440 ATTAAT AT^TAAT
Asp718I 1753 GGTACC G^GTACC
BamHI 8 GGATCC G^GATCC
BanII 1676 GGGCCC G[GA]GC[CT]^C
BbsI 605 GAAGAC GAAGAC(2/6)
BbvCI 2221 CCTCAGC CCTCAGC(-5/-2)
BcnI 533 CCCGG CC^[GC]GG
BlpI 91 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 91 GCTGAGC GC^T[ACGT]AGC
BpuAI 605 GAAGAC GAAGAC(2/6)
BsaAI 1812 CACGTA [CT]AC^GT[GA]
BsaI 359 GGTCTC GGTCTC(1/5)
BsmI 1964 GCATTC GAATGC(1/-1)
BspLU11I 396 ACATGT A^CATGT
BsrFI 974 GCCGGT [GA]^CCGG[CT]
BssSI 820 CACGAG CACGAG(-5/-1)
BstAPI 503 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1605 GGTGACC G^GT[ACGT]ACC
BstPI 1605 GGTGACC G^GT[ACGT]ACC
BstXI 1019 CCAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 91 GCTGAGC GC^T[ACGT]AGC
Cfr10I 974 GCCGGT [GA]^CCGG[CT]
EarI 191 GAAGAG CTCTTC(1/4)
EcoO65I 1605 GGTGACC G^GT[ACGT]ACC
HgaI 137 GCGTC GACGC(5/10)
HindIII 1542 AAGCTT A^AGCTT
HpaI 806 GTTAAC GTT^AAC
KpnI 1753 GGTACC GGTAC^C
Ksp632I 191 GAAGAG CTCTTC(1/4)
NciI 533 CCCGG CC^[GC]GG
NheI 2226 GCTAGC G^CTAGC
PshBI 2440 ATTAAT AT^TAAT
Psp1406I 50 AACGTT AA^CGTT
SapI 191 GAAGAGC GCTCTTC(1/4)
SphI 508 GCATGC GCATG^C
SspI 1165 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 977 GGTGCC G^G[CT][GA]CC
1753 GGTACC
BsmFI 706 GGGAC GGGAC(10/14)
44 GTCCC
BspHI 276 TCATGA T^CATGA
2426 TCATGA
BspMI 1513 GCAGGT ACCTGC(4/8)
2146 GCAGGT
DraII 706 GGGACCT [GA]G^G[ACGT]CC[CT]
1302 AGGACCT
DrdI 60 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
708 GACCTGACAGTC
EcoO109I 706 GGGACCT [GA]G^G[ACGT]CC[CT]
1302 AGGACCT
EcoRI 2015 GAATTC G^AATTC
2249 GAATTC
EcoRV 1638 GATATC GAT^ATC
1931 GATATC
FauI 1674 GCGGG CCCGC(4/6)
2381 GCGGG
HapII 534 CCGG C^CGG
975 CCGG
HincII 746 GTTGAC GT[CT]^[GA]AC
806 GTTAAC
HindII 746 GTTGAC GT[CT]^[GA]AC
806 GTTAAC
HpaII 534 CCGG C^CGG
975 CCGG
MspA1I 229 CCGCTG C[AC]G^C[GT]G
1601 CAGCGG
MspI 534 CCGG C^CGG
975 CCGG
NcoI 829 CCATGG C^CATGG
1129 CCATGG
PflMI 697 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1274 CCACACAATGG
PleI 2309 GACTC GAGTC(4/5)
590 GAGTC
PpuMI 706 GGGACCT [GA]G^G[AT]CC[CT]
1302 AGGACCT
RcaI 276 TCATGA T^CATGA
2426 TCATGA
Van91I 697 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1274 CCACACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BclI BfaI BglII BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV NsiI NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII