##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AscI AseI AsnI BbeI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BssSI BstBI
ClaI CpoI EagI EclXI Eco52I
EcoRV FseI Hin1I HindIII KasI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SfiI SfoI SfuI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 8 CGCG CG^CG
AflII 6812 CTTAAG C^TTAAG
AflIII 4776 ACATGT A^C[GA][CT]GT
AhdI 1643 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1643 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3369 TGCGCA TGC^GCA
AvrII 4414 CCTAGG C^CTAGG
BamHI 755 GGATCC G^GATCC
BbrPI 4046 CACGTG CAC^GTG
BciVI 259 GGATAC GTATCC(6/5)
BclI 3235 TGATCA T^GATCA
BfrI 6812 CTTAAG C^TTAAG
BlnI 4414 CCTAGG C^CTAGG
BsaAI 4046 CACGTG [CT]AC^GT[GA]
BspLU11I 4776 ACATGT A^CATGT
Bst1107I 6817 GTATAC GTA^TAC
BstUI 8 CGCG CG^CG
BstZ17I 6817 GTATAC GTA^TAC
DraI 5859 TTTAAA TTT^AAA
DraIII 632 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6995 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1643 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3235 TGATCA T^GATCA
FspI 3369 TGCGCA TGC^GCA
HpaI 3422 GTTAAC GTT^AAC
KspI 7 CCGCGG CCGC^GG
MvnI 8 CGCG CG^CG
NaeI 318 GCCGGC GCC^GGC
NgoMIV 318 GCCGGC G^CCGGC
NheI 6176 GCTAGC G^CTAGC
PaeR7I 1586 CTCGAG C^TCGAG
PmaCI 4046 CACGTG CAC^GTG
PmlI 4046 CACGTG CAC^GTG
SacII 7 CCGCGG CCGC^GG
SmaI 3516 CCCGGG CCC^GGG
SspI 1150 AATATT AAT^ATT
XhoI 1586 CTCGAG C^TCGAG
XmaCI 3516 CCCGGG C^CCGGG
XmaI 3516 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 4043 GTGCAC G^TGCAC
4677 GTGCAC
Aor51HI 529 AGCGCT AGC^GCT
5972 AGCGCT
ApaLI 4043 GTGCAC G^TGCAC
4677 GTGCAC
BsaBI 3230 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6786 GATTAAAATC
BsmI 6068 GAATGC GAATGC(1/-1)
6581 GCATTC
BsrFI 318 GCCGGC [GA]^CCGG[CT]
7456 GCCGGT
BstAPI 416 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2155 GCAGTGGCTGC
BstEII 4166 GGTGACC G^GT[ACGT]ACC
4669 GGTTACC
BstPI 4166 GGTGACC G^GT[ACGT]ACC
4669 GGTTACC
Cfr10I 318 GCCGGC [GA]^CCGG[CT]
7456 GCCGGT
Eco47III 529 AGCGCT AGC^GCT
5972 AGCGCT
EcoO65I 4166 GGTGACC G^GT[ACGT]ACC
4669 GGTTACC
EcoRI 170 GAATTC G^AATTC
3461 GAATTC
EcoT22I 3305 ATGCAT ATGCA^T
5271 ATGCAT
HgaI 1856 GCGTC GACGC(5/10)
3987 GCGTC
MamI 3230 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6786 GATTAAAATC
NdeI 4474 CATATG CA^TATG
6698 CATATG
NsiI 3305 ATGCAT ATGCA^T
5271 ATGCAT
SbfI 68 CCTGCAGG CCTGCA^GG
5934 CCTGCAGG
ScaI 773 AGTACT AGT^ACT
5757 AGTACT
SexAI 2530 ACCAGGT A^CC[AT]GGT
5484 ACCAGGT
SpeI 5134 ACTAGT A^CTAGT
5817 ACTAGT
Sse8387I 68 CCTGCAGG CCTGCA^GG
5934 CCTGCAGG
XcmI 657 CCATTGTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6315 CCATTGGGGTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I Asp718I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoII XmnI