##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AgeI Aor51HI AscI AspI AviII
AvrII BamHI BlnI BsiWI BspDI
BstEII BstPI ClaI Eco47III EcoNI
EcoO65I FseI FspI HindIII HpaI
MluI NdeI NheI NotI NruI
PacI PflFI PinAI PmeI PvuI
SalI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 6653 GACGTC GACGT^C
AccIII 2542 TCCGGA T^CCGGA
AclI 3911 AACGTT AA^CGTT
AflII 5988 CTTAAG C^TTAAG
Alw44I 6205 GTGCAC G^TGCAC
ApaLI 6205 GTGCAC G^TGCAC
AseI 5355 ATTAAT AT^TAAT
AsnI 5355 ATTAAT AT^TAAT
BbeI 173 GGCGCC GGCGC^C
BbvCI 241 CCTCAGC CCTCAGC(-5/-2)
BfrI 5988 CTTAAG C^TTAAG
BsaBI 1943 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2542 TCCGGA [AT]^CCGG[AT]
BseAI 2542 TCCGGA T^CCGGA
BsiEI 195 CGGCCG CG[GA][CT]^CG
BsmBI 327 GAGACG CGTCTC(1/5)
Bsp1407I 1378 TGTACA T^GTACA
BspEI 2542 TCCGGA T^CCGGA
BspMI 2482 ACCTGC ACCTGC(4/8)
BsrBI 567 GAGCGG CCGCTC(-3/-3)
BsrGI 1378 TGTACA T^GTACA
BssSI 2985 CACGAG CACGAG(-5/-1)
Bst1107I 7400 GTATAC GTA^TAC
BstBI 625 TTCGAA TT^CGAA
BstZ17I 7400 GTATAC GTA^TAC
CpoI 2515 CGGTCCG CG^G[AT]CCG
DraIII 3129 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 195 CGGCCG C^GGCCG
EclXI 195 CGGCCG C^GGCCG
Eco52I 195 CGGCCG C^GGCCG
KasI 173 GGCGCC G^GCGCC
KspI 258 CCGCGG CCGC^GG
MamI 1943 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1419 CAATTG C^AATTG
MroI 2542 TCCGGA T^CCGGA
MunI 1419 CAATTG C^AATTG
NarI 173 GGCGCC GG^CGCC
NspV 625 TTCGAA TT^CGAA
PshAI 5501 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5355 ATTAAT AT^TAAT
Psp1406I 3911 AACGTT AA^CGTT
RsrII 2515 CGGTCCG CG^G[AT]CCG
SacII 258 CCGCGG CCGC^GG
SapI 2075 GCTCTTC GCTCTTC(1/4)
SfoI 173 GGCGCC GGC^GCC
SfuI 625 TTCGAA TT^CGAA
SmaI 6964 CCCGGG CCC^GGG
SphI 5031 GCATGC GCATG^C
SspBI 1378 TGTACA T^GTACA
XbaI 5960 TCTAGA T^CTAGA
XmaCI 6964 CCCGGG C^CCGGG
XmaI 6964 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 173 GGCGCC G[GA]^CG[CT]C
6653 GACGTC
ApaI 5020 GGGCCC GGGCC^C
5152 GGGCCC
BalI 919 TGGCCA TGG^CCA
1801 TGGCCA
BbrPI 2744 CACGTG CAC^GTG
6608 CACGTG
BciVI 5171 GGATAC GTATCC(6/5)
798 GTATCC
BclI 2876 TGATCA T^GATCA
6351 TGATCA
BsaHI 173 GGCGCC G[GA]^CG[CT]C
6653 GACGTC
BssHII 158 GCGCGC G^CGCGC
412 GCGCGC
BstXI 181 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3039 CCAGCCTTCTGG
DrdI 329 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1718 GACTCGAGTGTC
FbaI 2876 TGATCA T^GATCA
6351 TGATCA
Hin1I 173 GGCGCC G[GA]^CG[CT]C
6653 GACGTC
MluNI 919 TGGCCA TGG^CCA
1801 TGGCCA
MscI 919 TGGCCA TGG^CCA
1801 TGGCCA
NaeI 170 GCCGGC GCC^GGC
6141 GCCGGC
NgoMIV 170 GCCGGC G^CCGGC
6141 GCCGGC
PmaCI 2744 CACGTG CAC^GTG
6608 CACGTG
PmlI 2744 CACGTG CAC^GTG
6608 CACGTG
SexAI 772 ACCTGGT A^CC[AT]GGT
993 ACCAGGT
StuI 3744 AGGCCT AGG^CCT
5301 AGGCCT
SwaI 467 ATTTAAAT ATTT^AAAT
4371 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApoI Asp700I Asp718I
AspEI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI Eco81I EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmnI