################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AvaII AviII AvrII BamHI BanII BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BspDI BspEI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HapII HpaII KpnI KspI MamI MluI MroI MspI NaeI NciI NcoI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AhdI 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 47 GTGCTC G[AT]GC[AT]^C AspI 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2223 CTCGGG C^[CT]CG[GA]G BciVI 2306 GGATAC GTATCC(6/5) BclI 349 TGATCA T^GATCA BlpI 1944 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1944 GCTAAGC GC^T[ACGT]AGC BsgI 703 GTGCAG GTGCAG(16/14) BsiHKAI 47 GTGCTC G[AT]GC[AT]^C BsmFI 1407 GTCCC GGGAC(10/14) BsoBI 2223 CTCGGG C^[CT]CG[GA]G BspHI 1225 TCATGA T^CATGA BspLU11I 2636 ACATGT A^CATGT BspMI 1998 GCAGGT ACCTGC(4/8) BsrBI 379 GAGCGG CCGCTC(-3/-3) BssSI 1264 CTCGTG CACGAG(-5/-1) BstYI 778 AGATCC [GA]^GATC[CT] CelII 1944 GCTAAGC GC^T[ACGT]AGC Cfr13I 1893 GGCCC G^G[ACGT]CC DsaI 52 CCGTGG C^C[GA][CT]GG Eam1105I 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1770 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 795 CCAAGG C^C[AT][AT]GG EcoT22I 2756 ATGCAT ATGCA^T FbaI 349 TGATCA T^GATCA HgaI 15 GACGC GACGC(5/10) HindIII 2175 AAGCTT A^AGCTT HpaI 2110 GTTAAC GTT^AAC MflI 778 AGATCC [GA]^GATC[CT] MspA1I 698 CAGCTG C[AC]G^C[GT]G NdeI 1645 CATATG CA^TATG NsiI 2756 ATGCAT ATGCA^T PflFI 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 795 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1286 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 698 CAGCTG CAG^CTG RcaI 1225 TCATGA T^CATGA SapI 1202 GCTCTTC GCTCTTC(1/4) Sau96I 1893 GGCCC G^G[ACGT]CC SphI 2421 GCATGC GCATG^C SspI 1669 AATATT AAT^ATT StuI 927 AGGCCT AGG^CCT StyI 795 CCAAGG C^C[AT][AT]GG Tth111I 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 795 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 778 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 1 CGCG CG^CG 17 CGCG AcyI 501 GGCGCC G[GA]^CG[CT]C 750 GGCGCC AflIII 1336 ACGTGT A^C[GA][CT]GT 2636 ACATGT AlwI 779 GATCC GGATC(4/5) 1113 GGATC AseI 1721 ATTAAT AT^TAAT 2377 ATTAAT AsnI 1721 ATTAAT AT^TAAT 2377 ATTAAT BalI 316 TGGCCA TGG^CCA 1416 TGGCCA BbeI 501 GGCGCC GGCGC^C 750 GGCGCC BmyI 47 GTGCTC G[AGT]GC[ACT]^C 85 GTGCCC BsaAI 1172 CACGTA [CT]AC^GT[GA] 1335 TACGTG BsaHI 501 GGCGCC G[GA]^CG[CT]C 750 GGCGCC BseRI 333 CTCCTC GAGGAG(10/8) 738 CTCCTC BsmI 444 GCATTC GAATGC(1/-1) 2758 GCATTC Bsp1286I 47 GTGCTC G[AGT]GC[ACT]^C 85 GTGCCC Bsp1407I 1641 TGTACA T^GTACA 2355 TGTACA BsrGI 1641 TGTACA T^GTACA 2355 TGTACA BstUI 1 CGCG CG^CG 17 CGCG EarI 1203 CTCTTC CTCTTC(1/4) 1307 CTCTTC FauI 2047 CCCGC CCCGC(4/6) 498 GCGGG HaeII 501 GGCGCC [GA]GCGC^[CT] 750 GGCGCC Hin1I 501 GGCGCC G[GA]^CG[CT]C 750 GGCGCC KasI 501 GGCGCC G^GCGCC 750 GGCGCC Ksp632I 1203 CTCTTC CTCTTC(1/4) 1307 CTCTTC MaeII 1173 ACGT A^CGT 1336 ACGT MfeI 1241 CAATTG C^AATTG 2540 CAATTG MluNI 316 TGGCCA TGG^CCA 1416 TGGCCA MscI 316 TGGCCA TGG^CCA 1416 TGGCCA MunI 1241 CAATTG C^AATTG 2540 CAATTG MvnI 1 CGCG CG^CG 17 CGCG NarI 501 GGCGCC GG^CGCC 750 GGCGCC PacI 1722 TTAATTAA TTAAT^TAA 2580 TTAATTAA PshBI 1721 ATTAAT AT^TAAT 2377 ATTAAT SfoI 501 GGCGCC GGC^GCC 750 GGCGCC SpeI 527 ACTAGT A^CTAGT 1346 ACTAGT SspBI 1641 TGTACA T^GTACA 2355 TGTACA TaqI 1017 TCGA T^CGA 2556 TCGA TthHB8I 1017 TCGA T^CGA 2556 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccI AciI AcsI AfaI AluI ApoI BanI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I CfoI DdeI DpnII DraI EaeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI