##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AvaII AviII AvrII BamHI BanII
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BpmI
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BspDI
BspEI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FseI FspI HapII
HpaII KpnI KspI MamI MluI
MroI MspI NaeI NciI NcoI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AhdI 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 47 GTGCTC G[AT]GC[AT]^C
AspI 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2223 CTCGGG C^[CT]CG[GA]G
BciVI 2306 GGATAC GTATCC(6/5)
BclI 349 TGATCA T^GATCA
BlpI 1944 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1944 GCTAAGC GC^T[ACGT]AGC
BsgI 703 GTGCAG GTGCAG(16/14)
BsiHKAI 47 GTGCTC G[AT]GC[AT]^C
BsmFI 1407 GTCCC GGGAC(10/14)
BsoBI 2223 CTCGGG C^[CT]CG[GA]G
BspHI 1225 TCATGA T^CATGA
BspLU11I 2636 ACATGT A^CATGT
BspMI 1998 GCAGGT ACCTGC(4/8)
BsrBI 379 GAGCGG CCGCTC(-3/-3)
BssSI 1264 CTCGTG CACGAG(-5/-1)
BstYI 778 AGATCC [GA]^GATC[CT]
CelII 1944 GCTAAGC GC^T[ACGT]AGC
Cfr13I 1893 GGCCC G^G[ACGT]CC
DsaI 52 CCGTGG C^C[GA][CT]GG
Eam1105I 942 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1770 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 795 CCAAGG C^C[AT][AT]GG
EcoT22I 2756 ATGCAT ATGCA^T
FbaI 349 TGATCA T^GATCA
HgaI 15 GACGC GACGC(5/10)
HindIII 2175 AAGCTT A^AGCTT
HpaI 2110 GTTAAC GTT^AAC
MflI 778 AGATCC [GA]^GATC[CT]
MspA1I 698 CAGCTG C[AC]G^C[GT]G
NdeI 1645 CATATG CA^TATG
NsiI 2756 ATGCAT ATGCA^T
PflFI 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 795 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1286 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 698 CAGCTG CAG^CTG
RcaI 1225 TCATGA T^CATGA
SapI 1202 GCTCTTC GCTCTTC(1/4)
Sau96I 1893 GGCCC G^G[ACGT]CC
SphI 2421 GCATGC GCATG^C
SspI 1669 AATATT AAT^ATT
StuI 927 AGGCCT AGG^CCT
StyI 795 CCAAGG C^C[AT][AT]GG
Tth111I 848 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 795 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 778 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1 CGCG CG^CG
17 CGCG
AcyI 501 GGCGCC G[GA]^CG[CT]C
750 GGCGCC
AflIII 1336 ACGTGT A^C[GA][CT]GT
2636 ACATGT
AlwI 779 GATCC GGATC(4/5)
1113 GGATC
AseI 1721 ATTAAT AT^TAAT
2377 ATTAAT
AsnI 1721 ATTAAT AT^TAAT
2377 ATTAAT
BalI 316 TGGCCA TGG^CCA
1416 TGGCCA
BbeI 501 GGCGCC GGCGC^C
750 GGCGCC
BmyI 47 GTGCTC G[AGT]GC[ACT]^C
85 GTGCCC
BsaAI 1172 CACGTA [CT]AC^GT[GA]
1335 TACGTG
BsaHI 501 GGCGCC G[GA]^CG[CT]C
750 GGCGCC
BseRI 333 CTCCTC GAGGAG(10/8)
738 CTCCTC
BsmI 444 GCATTC GAATGC(1/-1)
2758 GCATTC
Bsp1286I 47 GTGCTC G[AGT]GC[ACT]^C
85 GTGCCC
Bsp1407I 1641 TGTACA T^GTACA
2355 TGTACA
BsrGI 1641 TGTACA T^GTACA
2355 TGTACA
BstUI 1 CGCG CG^CG
17 CGCG
EarI 1203 CTCTTC CTCTTC(1/4)
1307 CTCTTC
FauI 2047 CCCGC CCCGC(4/6)
498 GCGGG
HaeII 501 GGCGCC [GA]GCGC^[CT]
750 GGCGCC
Hin1I 501 GGCGCC G[GA]^CG[CT]C
750 GGCGCC
KasI 501 GGCGCC G^GCGCC
750 GGCGCC
Ksp632I 1203 CTCTTC CTCTTC(1/4)
1307 CTCTTC
MaeII 1173 ACGT A^CGT
1336 ACGT
MfeI 1241 CAATTG C^AATTG
2540 CAATTG
MluNI 316 TGGCCA TGG^CCA
1416 TGGCCA
MscI 316 TGGCCA TGG^CCA
1416 TGGCCA
MunI 1241 CAATTG C^AATTG
2540 CAATTG
MvnI 1 CGCG CG^CG
17 CGCG
NarI 501 GGCGCC GG^CGCC
750 GGCGCC
PacI 1722 TTAATTAA TTAAT^TAA
2580 TTAATTAA
PshBI 1721 ATTAAT AT^TAAT
2377 ATTAAT
SfoI 501 GGCGCC GGC^GCC
750 GGCGCC
SpeI 527 ACTAGT A^CTAGT
1346 ACTAGT
SspBI 1641 TGTACA T^GTACA
2355 TGTACA
TaqI 1017 TCGA T^CGA
2556 TCGA
TthHB8I 1017 TCGA T^CGA
2556 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI BanI BbvI BfaI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I CfoI DdeI DpnII
DraI EaeI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI