################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvaI AviII BamHI BbeI BfrI BglII BlpI BpmI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstZ17I CelII Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 1981 CGCG CG^CG ApaI 884 GGGCCC GGGCC^C Asp700I 443 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1510 CCTAGG C^CTAGG BbvCI 655 CCTCAGC CCTCAGC(-5/-2) BciVI 1557 GGATAC GTATCC(6/5) BclI 28 TGATCA T^GATCA BlnI 1510 CCTAGG C^CTAGG BsaWI 1444 ACCGGA [AT]^CCGG[AT] BspDI 120 ATCGAT AT^CGAT BspHI 1935 TCATGA T^CATGA BsrDI 1811 GCAATG GCAATG(2/0) BssSI 1583 CTCGTG CACGAG(-5/-1) BstBI 682 TTCGAA TT^CGAA BstEII 326 GGTGACC G^GT[ACGT]ACC BstPI 326 GGTGACC G^GT[ACGT]ACC BstUI 1981 CGCG CG^CG BstXI 748 CCATGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1990 GGATCT [GA]^GATC[CT] ClaI 120 ATCGAT AT^CGAT EcoO65I 326 GGTGACC G^GT[ACGT]ACC EcoRI 447 GAATTC G^AATTC FbaI 28 TGATCA T^GATCA MflI 1990 GGATCT [GA]^GATC[CT] MseI 2013 TTAA T^TAA MvnI 1981 CGCG CG^CG NheI 1095 GCTAGC G^CTAGC NspV 682 TTCGAA TT^CGAA PflMI 1798 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 356 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1935 TCATGA T^CATGA SfuI 682 TTCGAA TT^CGAA SmlI 1077 CTCAAG C^T[CT][GA]AG SphI 70 GCATGC GCATG^C StuI 493 AGGCCT AGG^CCT Tru9I 2013 TTAA T^TAA Van91I 1798 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 733 CCACGGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1990 GGATCT [GA]^GATC[CT] XmnI 443 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 651 GGTACC G^GTACC 1616 GGTACC AcsI 177 GAATTT [GA]^AATT[CT] 447 GAATTC AflIII 64 ACACGT A^C[GA][CT]GT 292 ACGTGT Alw44I 556 GTGCAC G^TGCAC 568 GTGCAC ApaLI 556 GTGCAC G^TGCAC 568 GTGCAC ApoI 177 GAATTT [GA]^AATT[CT] 447 GAATTC Asp718I 651 GGTACC G^GTACC 1616 GGTACC AspI 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1367 GACTATGTC BalI 745 TGGCCA TGG^CCA 1743 TGGCCA BbrPI 57 CACGTG CAC^GTG 65 CACGTG BbsI 1594 GAAGAC GAAGAC(2/6) 779 GTCTTC BcgI 1338 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 694 GCAACAGCCTCG BcnI 401 CCGGG CC^[GC]GG 785 CCCGG BglI 1842 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1971 GCCTCCGAGGC BpuAI 1594 GAAGAC GAAGAC(2/6) 779 GTCTTC BsaAI 57 CACGTG [CT]AC^GT[GA] 65 CACGTG BsgI 716 CTGCAC GTGCAG(16/14) 1380 GTGCAG BstAPI 545 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1326 GCAGATTCTGC DraIII 54 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 969 CACCCCGTG KpnI 651 GGTACC GGTAC^C 1616 GGTACC MluNI 745 TGGCCA TGG^CCA 1743 TGGCCA MscI 745 TGGCCA TGG^CCA 1743 TGGCCA MslI 65 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 749 CATGGTTGTG NciI 401 CCGGG CC^[GC]GG 785 CCCGG NspI 70 GCATGC [GA]CATG^[CT] 1697 GCATGT PflFI 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1367 GACTATGTC PmaCI 57 CACGTG CAC^GTG 65 CACGTG PmlI 57 CACGTG CAC^GTG 65 CACGTG Tth111I 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1367 GACTATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaII BanI BanII BbvI BfaI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I