##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AvaI AviII
BamHI BbeI BfrI BglII BlpI
BpmI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstZ17I CelII Cfr10I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 1981 CGCG CG^CG
ApaI 884 GGGCCC GGGCC^C
Asp700I 443 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1510 CCTAGG C^CTAGG
BbvCI 655 CCTCAGC CCTCAGC(-5/-2)
BciVI 1557 GGATAC GTATCC(6/5)
BclI 28 TGATCA T^GATCA
BlnI 1510 CCTAGG C^CTAGG
BsaWI 1444 ACCGGA [AT]^CCGG[AT]
BspDI 120 ATCGAT AT^CGAT
BspHI 1935 TCATGA T^CATGA
BsrDI 1811 GCAATG GCAATG(2/0)
BssSI 1583 CTCGTG CACGAG(-5/-1)
BstBI 682 TTCGAA TT^CGAA
BstEII 326 GGTGACC G^GT[ACGT]ACC
BstPI 326 GGTGACC G^GT[ACGT]ACC
BstUI 1981 CGCG CG^CG
BstXI 748 CCATGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1990 GGATCT [GA]^GATC[CT]
ClaI 120 ATCGAT AT^CGAT
EcoO65I 326 GGTGACC G^GT[ACGT]ACC
EcoRI 447 GAATTC G^AATTC
FbaI 28 TGATCA T^GATCA
MflI 1990 GGATCT [GA]^GATC[CT]
MseI 2013 TTAA T^TAA
MvnI 1981 CGCG CG^CG
NheI 1095 GCTAGC G^CTAGC
NspV 682 TTCGAA TT^CGAA
PflMI 1798 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 356 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1935 TCATGA T^CATGA
SfuI 682 TTCGAA TT^CGAA
SmlI 1077 CTCAAG C^T[CT][GA]AG
SphI 70 GCATGC GCATG^C
StuI 493 AGGCCT AGG^CCT
Tru9I 2013 TTAA T^TAA
Van91I 1798 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 733 CCACGGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1990 GGATCT [GA]^GATC[CT]
XmnI 443 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 651 GGTACC G^GTACC
1616 GGTACC
AcsI 177 GAATTT [GA]^AATT[CT]
447 GAATTC
AflIII 64 ACACGT A^C[GA][CT]GT
292 ACGTGT
Alw44I 556 GTGCAC G^TGCAC
568 GTGCAC
ApaLI 556 GTGCAC G^TGCAC
568 GTGCAC
ApoI 177 GAATTT [GA]^AATT[CT]
447 GAATTC
Asp718I 651 GGTACC G^GTACC
1616 GGTACC
AspI 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1367 GACTATGTC
BalI 745 TGGCCA TGG^CCA
1743 TGGCCA
BbrPI 57 CACGTG CAC^GTG
65 CACGTG
BbsI 1594 GAAGAC GAAGAC(2/6)
779 GTCTTC
BcgI 1338 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
694 GCAACAGCCTCG
BcnI 401 CCGGG CC^[GC]GG
785 CCCGG
BglI 1842 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1971 GCCTCCGAGGC
BpuAI 1594 GAAGAC GAAGAC(2/6)
779 GTCTTC
BsaAI 57 CACGTG [CT]AC^GT[GA]
65 CACGTG
BsgI 716 CTGCAC GTGCAG(16/14)
1380 GTGCAG
BstAPI 545 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1326 GCAGATTCTGC
DraIII 54 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
969 CACCCCGTG
KpnI 651 GGTACC GGTAC^C
1616 GGTACC
MluNI 745 TGGCCA TGG^CCA
1743 TGGCCA
MscI 745 TGGCCA TGG^CCA
1743 TGGCCA
MslI 65 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
749 CATGGTTGTG
NciI 401 CCGGG CC^[GC]GG
785 CCCGG
NspI 70 GCATGC [GA]CATG^[CT]
1697 GCATGT
PflFI 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1367 GACTATGTC
PmaCI 57 CACGTG CAC^GTG
65 CACGTG
PmlI 57 CACGTG CAC^GTG
65 CACGTG
Tth111I 773 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1367 GACTATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BanI BanII
BbvI BfaI BmyI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I