##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AhdI AscI AseI AsnI AspEI
AviII BbeI BbrPI BsiWI BssHII
BstBI CpoI EagI Eam1105I EclXI
Eco52I FseI FspI KasI KspI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3505 GACGTC GACGT^C
AclI 5582 AACGTT AA^CGTT
AgeI 1640 ACCGGT A^CCGGT
Aor51HI 3119 AGCGCT AGC^GCT
ApaI 3479 GGGCCC GGGCC^C
AspI 7939 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1287 CCTAGG C^CTAGG
BglII 4872 AGATCT A^GATCT
BlnI 1287 CCTAGG C^CTAGG
BsaAI 7010 TACGTG [CT]AC^GT[GA]
BsiEI 3300 CGGTCG CG[GA][CT]^CG
BsmBI 1006 CGTCTC CGTCTC(1/5)
BspDI 7811 ATCGAT AT^CGAT
BsrFI 1640 ACCGGT [GA]^CCGG[CT]
Cfr10I 1640 ACCGGT [GA]^CCGG[CT]
ClaI 7811 ATCGAT AT^CGAT
Eco47III 3119 AGCGCT AGC^GCT
HindIII 2846 AAGCTT A^AGCTT
HpaI 4203 GTTAAC GTT^AAC
MluI 4199 ACGCGT A^CGCGT
PflFI 7939 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1640 ACCGGT A^CCGGT
PshAI 7077 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5582 AACGTT AA^CGTT
SmaI 6518 CCCGGG CCC^GGG
SpeI 2331 ACTAGT A^CTAGT
SspI 7676 AATATT AAT^ATT
Tth111I 7939 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 6518 CCCGGG C^CCGGG
XmaI 6518 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 4200 CGCG CG^CG
7028 CGCG
AccIII 7085 TCCGGA T^CCGGA
7715 TCCGGA
AcyI 692 GACGCC G[GA]^CG[CT]C
3505 GACGTC
AflII 1137 CTTAAG C^TTAAG
6532 CTTAAG
Alw44I 3824 GTGCAC G^TGCAC
9232 GTGCAC
ApaLI 3824 GTGCAC G^TGCAC
9232 GTGCAC
BamHI 1155 GGATCC G^GATCC
4575 GGATCC
BfrI 1137 CTTAAG C^TTAAG
6532 CTTAAG
BsaHI 692 GACGCC G[GA]^CG[CT]C
3505 GACGTC
BseAI 7085 TCCGGA T^CCGGA
7715 TCCGGA
BspEI 7085 TCCGGA T^CCGGA
7715 TCCGGA
BsrBI 534 CCGCTC CCGCTC(-3/-3)
7357 GAGCGG
BssSI 4490 CTCGTG CACGAG(-5/-1)
6500 CTCGTG
BstEII 7936 GGTGACC G^GT[ACGT]ACC
7944 GGTCACC
BstPI 7936 GGTGACC G^GT[ACGT]ACC
7944 GGTCACC
BstUI 4200 CGCG CG^CG
7028 CGCG
DraI 7269 TTTAAA TTT^AAA
8872 TTTAAA
DraIII 7931 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
8926 CACCATGTG
DrdI 7657 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9073 GACCTGTTTGTC
EcoO65I 7936 GGTGACC G^GT[ACGT]ACC
7944 GGTCACC
EcoRI 3706 GAATTC G^AATTC
4496 GAATTC
Hin1I 692 GACGCC G[GA]^CG[CT]C
3505 GACGTC
MroI 7085 TCCGGA T^CCGGA
7715 TCCGGA
MvnI 4200 CGCG CG^CG
7028 CGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NsiI NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmlI SphI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI