##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AscI AseI AsnI Asp700I
AspI BbeI BcgI BclI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BssHII BstBI ClaI DrdI
EcoRI FbaI HpaI KasI MamI
MfeI MroI MunI NarI NheI
NotI NruI NspV PacI PflFI
PmeI PshBI PvuI SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 4596 AACGTT AA^CGTT
AgeI 2490 ACCGGT A^CCGGT
AhdI 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5335 AGCGCT AGC^GCT
AspEI 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 585 TGCGCA TGC^GCA
BbrPI 3240 CACGTG CAC^GTG
BstAPI 5498 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 194 GGTTACC G^GT[ACGT]ACC
BstPI 194 GGTTACC G^GT[ACGT]ACC
CpoI 2350 CGGACCG CG^G[AT]CCG
DraI 5278 TTTAAA TTT^AAA
EagI 1648 CGGCCG C^GGCCG
Eam1105I 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1648 CGGCCG C^GGCCG
Eco47III 5335 AGCGCT AGC^GCT
Eco52I 1648 CGGCCG C^GGCCG
EcoO65I 194 GGTTACC G^GT[ACGT]ACC
EcoRV 6503 GATATC GAT^ATC
FseI 1649 GGCCGGCC GGCCGG^CC
FspI 585 TGCGCA TGC^GCA
HincII 6608 GTTGAC GT[CT]^[GA]AC
HindII 6608 GTTGAC GT[CT]^[GA]AC
KspI 2354 CCGCGG CCGC^GG
MluI 2364 ACGCGT A^CGCGT
NdeI 1599 CATATG CA^TATG
PaeR7I 1698 CTCGAG C^TCGAG
PinAI 2490 ACCGGT A^CCGGT
PmaCI 3240 CACGTG CAC^GTG
PmlI 3240 CACGTG CAC^GTG
Psp1406I 4596 AACGTT AA^CGTT
RsrII 2350 CGGACCG CG^G[AT]CCG
SacI 758 GAGCTC GAGCT^C
SacII 2354 CCGCGG CCGC^GG
SbfI 3335 CCTGCAGG CCTGCA^GG
Sse8387I 3335 CCTGCAGG CCTGCA^GG
SspI 4918 AATATT AAT^ATT
XbaI 6346 TCTAGA T^CTAGA
XhoI 1698 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 2327 GACGTC GACGT^C
4115 GACGTC
AccI 4428 GTATAC GT^[AC][GT]AC
6067 GTATAC
AflII 4694 CTTAAG C^TTAAG
6192 CTTAAG
AvrII 3727 CCTAGG C^CTAGG
6452 CCTAGG
BciVI 1351 GTATCC GTATCC(6/5)
6591 GTATCC
BfrI 4694 CTTAAG C^TTAAG
6192 CTTAAG
BlnI 3727 CCTAGG C^CTAGG
6452 CCTAGG
BsiEI 1648 CGGCCG CG[GA][CT]^CG
2488 CGACCG
BsrBI 2482 CCGCTC CCGCTC(-3/-3)
1267 GAGCGG
Bst1107I 4428 GTATAC GTA^TAC
6067 GTATAC
BstZ17I 4428 GTATAC GTA^TAC
6067 GTATAC
PshAI 2729 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7360 GACCCGAGTC
SapI 2273 GCTCTTC GCTCTTC(1/4)
3876 GCTCTTC
SphI 4267 GCATGC GCATG^C
5238 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI