################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AccIII AscI AseI AsnI Asp700I AspI BbeI BcgI BclI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BssHII BstBI ClaI DrdI EcoRI FbaI HpaI KasI MamI MfeI MroI MunI NarI NheI NotI NruI NspV PacI PflFI PmeI PshBI PvuI SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 4596 AACGTT AA^CGTT AgeI 2490 ACCGGT A^CCGGT AhdI 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 5335 AGCGCT AGC^GCT AspEI 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 585 TGCGCA TGC^GCA BbrPI 3240 CACGTG CAC^GTG BstAPI 5498 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 194 GGTTACC G^GT[ACGT]ACC BstPI 194 GGTTACC G^GT[ACGT]ACC CpoI 2350 CGGACCG CG^G[AT]CCG DraI 5278 TTTAAA TTT^AAA EagI 1648 CGGCCG C^GGCCG Eam1105I 740 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1648 CGGCCG C^GGCCG Eco47III 5335 AGCGCT AGC^GCT Eco52I 1648 CGGCCG C^GGCCG EcoO65I 194 GGTTACC G^GT[ACGT]ACC EcoRV 6503 GATATC GAT^ATC FseI 1649 GGCCGGCC GGCCGG^CC FspI 585 TGCGCA TGC^GCA HincII 6608 GTTGAC GT[CT]^[GA]AC HindII 6608 GTTGAC GT[CT]^[GA]AC KspI 2354 CCGCGG CCGC^GG MluI 2364 ACGCGT A^CGCGT NdeI 1599 CATATG CA^TATG PaeR7I 1698 CTCGAG C^TCGAG PinAI 2490 ACCGGT A^CCGGT PmaCI 3240 CACGTG CAC^GTG PmlI 3240 CACGTG CAC^GTG Psp1406I 4596 AACGTT AA^CGTT RsrII 2350 CGGACCG CG^G[AT]CCG SacI 758 GAGCTC GAGCT^C SacII 2354 CCGCGG CCGC^GG SbfI 3335 CCTGCAGG CCTGCA^GG Sse8387I 3335 CCTGCAGG CCTGCA^GG SspI 4918 AATATT AAT^ATT XbaI 6346 TCTAGA T^CTAGA XhoI 1698 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AatII 2327 GACGTC GACGT^C 4115 GACGTC AccI 4428 GTATAC GT^[AC][GT]AC 6067 GTATAC AflII 4694 CTTAAG C^TTAAG 6192 CTTAAG AvrII 3727 CCTAGG C^CTAGG 6452 CCTAGG BciVI 1351 GTATCC GTATCC(6/5) 6591 GTATCC BfrI 4694 CTTAAG C^TTAAG 6192 CTTAAG BlnI 3727 CCTAGG C^CTAGG 6452 CCTAGG BsiEI 1648 CGGCCG CG[GA][CT]^CG 2488 CGACCG BsrBI 2482 CCGCTC CCGCTC(-3/-3) 1267 GAGCGG Bst1107I 4428 GTATAC GTA^TAC 6067 GTATAC BstZ17I 4428 GTATAC GTA^TAC 6067 GTATAC PshAI 2729 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 7360 GACCCGAGTC SapI 2273 GCTCTTC GCTCTTC(1/4) 3876 GCTCTTC SphI 4267 GCATGC GCATG^C 5238 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 155 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI