##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccII AccIII AgeI AscI AspI
BamHI BbrPI BcgI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraIII
EagI EclXI Eco52I EcoO65I EcoRV
FseI KspI MluI MroI MvnI
NaeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SphI Sse8387I Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4132 GACGTC GACGT^C
Acc65I 891 GGTACC G^GTACC
AclI 8514 AACGTT AA^CGTT
AflII 6736 CTTAAG C^TTAAG
AhdI 3442 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 558 AGCGCT AGC^GCT
ApaI 1782 GGGCCC GGGCC^C
Asp700I 3876 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 891 GGTACC G^GTACC
AspEI 3442 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 8298 TGCGCA TGC^GCA
BbeI 2496 GGCGCC GGCGC^C
BbvCI 499 GCTGAGG CCTCAGC(-5/-2)
BfrI 6736 CTTAAG C^TTAAG
BglI 337 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2888 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 1506 CCGCTC CCGCTC(-3/-3)
BssSI 987 CACGAG CACGAG(-5/-1)
Bst1107I 5543 GTATAC GTA^TAC
BstZ17I 5543 GTATAC GTA^TAC
DrdI 2682 GACCAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3442 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 558 AGCGCT AGC^GCT
FauI 6488 CCCGC CCCGC(4/6)
FspI 8298 TGCGCA TGC^GCA
HgaI 639 GCGTC GACGC(5/10)
KasI 2496 GGCGCC G^GCGCC
KpnI 891 GGTACC GGTAC^C
MamI 2888 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7182 CAATTG C^AATTG
MunI 7182 CAATTG C^AATTG
NarI 2496 GGCGCC GG^CGCC
NheI 1841 GCTAGC G^CTAGC
Psp1406I 8514 AACGTT AA^CGTT
SfoI 2496 GGCGCC GGC^GCC
SnaBI 623 TACGTA TAC^GTA
StuI 4167 AGGCCT AGG^CCT
SwaI 8226 ATTTAAAT ATTT^AAAT
XmnI 3876 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 2496 GGCGCC G[GA]^CG[CT]C
4132 GACGTC
Alw44I 4544 GTGCAC G^TGCAC
8101 GTGCAC
AocI 721 CCTGAGG CC^T[ACGT]AGG
872 CCTCAGG
ApaLI 4544 GTGCAC G^TGCAC
8101 GTGCAC
AseI 367 ATTAAT AT^TAAT
6729 ATTAAT
AsnI 367 ATTAAT AT^TAAT
6729 ATTAAT
AvaI 634 CTCGAG C^[CT]CG[GA]G
896 CTCGAG
AvrII 6711 CCTAGG C^CTAGG
8114 CCTAGG
BalI 6871 TGGCCA TGG^CCA
7838 TGGCCA
BcnI 1220 CCGGG CC^[GC]GG
4220 CCCGG
BglII 1427 AGATCT A^GATCT
5914 AGATCT
BlnI 6711 CCTAGG C^CTAGG
8114 CCTAGG
BlpI 346 GCTGAGC GC^T[ACGT]AGC
1811 GCTCAGC
Bpu1102I 346 GCTGAGC GC^T[ACGT]AGC
1811 GCTCAGC
BsaAI 623 TACGTA [CT]AC^GT[GA]
4229 CACGTA
BsaHI 2496 GGCGCC G[GA]^CG[CT]C
4132 GACGTC
BsoBI 634 CTCGAG C^[CT]CG[GA]G
896 CTCGAG
BspHI 6836 TCATGA T^CATGA
8305 TCATGA
BstAPI 7375 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7598 GCATTGGCTGC
Bsu36I 721 CCTGAGG CC^T[ACGT]AGG
872 CCTCAGG
CelII 346 GCTGAGC GC^T[ACGT]AGC
1811 GCTCAGC
EaeI 6871 TGGCCA [CT]^GGCC[GA]
7838 TGGCCA
Eco81I 721 CCTGAGG CC^T[ACGT]AGG
872 CCTCAGG
EcoNI 1797 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6138 CCTATTGCAGG
HaeII 558 AGCGCT [GA]GCGC^[CT]
2496 GGCGCC
HapII 1220 CCGG C^CGG
4221 CCGG
Hin1I 2496 GGCGCC G[GA]^CG[CT]C
4132 GACGTC
HindIII 824 AAGCTT A^AGCTT
3195 AAGCTT
HpaII 1220 CCGG C^CGG
4221 CCGG
MluNI 6871 TGGCCA TGG^CCA
7838 TGGCCA
MscI 6871 TGGCCA TGG^CCA
7838 TGGCCA
MspI 1220 CCGG C^CGG
4221 CCGG
NciI 1220 CCGGG CC^[GC]GG
4220 CCCGG
NdeI 1143 CATATG CA^TATG
5822 CATATG
PaeR7I 634 CTCGAG C^TCGAG
896 CTCGAG
PflMI 3765 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4303 CCAAACATTGG
PshBI 367 ATTAAT AT^TAAT
6729 ATTAAT
RcaI 6836 TCATGA T^CATGA
8305 TCATGA
SspI 2406 AATATT AAT^ATT
7493 AATATT
Van91I 3765 CCAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4303 CCAAACATTGG
XbaI 4107 TCTAGA T^CTAGA
8678 TCTAGA
XhoI 634 CTCGAG C^TCGAG
896 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BciVI BclI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SpeI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII