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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AgeI Aor51HI ApaI
AscI AvaI AvrII BbeI BlnI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI HpaI KasI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2271 GACGTC GACGT^C
AflII 8459 CTTAAG C^TTAAG
Alw44I 4929 GTGCAC G^TGCAC
ApaLI 4929 GTGCAC G^TGCAC
AviII 587 TGCGCA TGC^GCA
BbsI 240 GAAGAC GAAGAC(2/6)
BcgI 2853 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7131 GGATAC GTATCC(6/5)
BclI 1617 TGATCA T^GATCA
BfrI 8459 CTTAAG C^TTAAG
BglI 5620 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3197 AGATCT A^GATCT
BlpI 3182 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3182 GCTCAGC GC^T[ACGT]AGC
BpuAI 240 GAAGAC GAAGAC(2/6)
BsaBI 1276 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 8106 ACATGT A^CATGT
BsrFI 65 ACCGGC [GA]^CCGG[CT]
CelII 3182 GCTCAGC GC^T[ACGT]AGC
Cfr10I 65 ACCGGC [GA]^CCGG[CT]
DraII 6029 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 8007 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4186 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 6029 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 7 CCCGC CCCGC(4/6)
FbaI 1617 TGATCA T^GATCA
FspI 587 TGCGCA TGC^GCA
HaeII 5479 AGCGCC [GA]GCGC^[CT]
HindIII 8636 AAGCTT A^AGCTT
MamI 1276 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 380 CAATTG C^AATTG
MunI 380 CAATTG C^AATTG
NcoI 2019 CCATGG C^CATGG
NheI 8138 GCTAGC G^CTAGC
PacI 5487 TTAATTAA TTAAT^TAA
PflMI 3083 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 6029 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 8162 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 375 GAGCTC GAGCT^C
SbfI 5533 CCTGCAGG CCTGCA^GG
SexAI 2156 ACCAGGT A^CC[AT]GGT
Sse8387I 5533 CCTGCAGG CCTGCA^GG
StuI 1069 AGGCCT AGG^CCT
Van91I 3083 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 2271 GACGTC G[GA]^CG[CT]C
7537 GACGCC
AflIII 4933 ACACGT A^C[GA][CT]GT
8106 ACATGT
AhdI 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6031 GACCCTTAGTC
AlwNI 1538 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3422 CAGATGCTG
AocI 4639 CCTAAGG CC^T[ACGT]AGG
5768 CCTCAGG
AseI 6282 ATTAAT AT^TAAT
8156 ATTAAT
AsnI 6282 ATTAAT AT^TAAT
8156 ATTAAT
AspEI 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6031 GACCCTTAGTC
AspI 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGCGTC
BalI 2952 TGGCCA TGG^CCA
5004 TGGCCA
BanII 3 GAGCCC G[GA]GC[CT]^C
375 GAGCTC
BbrPI 4934 CACGTG CAC^GTG
8323 CACGTG
BbvCI 2839 CCTCAGC CCTCAGC(-5/-2)
6291 GCTGAGG
BcnI 28 CCGGG CC^[GC]GG
85 CCCGG
BsaAI 4934 CACGTG [CT]AC^GT[GA]
8323 CACGTG
BsaHI 2271 GACGTC G[GA]^CG[CT]C
7537 GACGCC
BsmI 3302 GAATGC GAATGC(1/-1)
5841 GAATGC
BssSI 1318 CACGAG CACGAG(-5/-1)
3002 CACGAG
Bsu36I 4639 CCTAAGG CC^T[ACGT]AGG
5768 CCTCAGG
DsaI 2019 CCATGG C^C[GA][CT]GG
2748 CCACGG
Eam1105I 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6031 GACCCTTAGTC
Eco81I 4639 CCTAAGG CC^T[ACGT]AGG
5768 CCTCAGG
EcoNI 501 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6021 CCTTAGAAAGG
EcoT22I 5197 ATGCAT ATGCA^T
7071 ATGCAT
Hin1I 2271 GACGTC G[GA]^CG[CT]C
7537 GACGCC
MluNI 2952 TGGCCA TGG^CCA
5004 TGGCCA
MscI 2952 TGGCCA TGG^CCA
5004 TGGCCA
NciI 28 CCGGG CC^[GC]GG
85 CCCGG
NsiI 5197 ATGCAT ATGCA^T
7071 ATGCAT
PflFI 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGCGTC
PmaCI 4934 CACGTG CAC^GTG
8323 CACGTG
PmlI 4934 CACGTG CAC^GTG
8323 CACGTG
PshBI 6282 ATTAAT AT^TAAT
8156 ATTAAT
PstI 2342 CTGCAG CTGCA^G
5534 CTGCAG
SapI 570 GAAGAGC GCTCTTC(1/4)
3214 GAAGAGC
Tth111I 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4211 GACAGCGTC
XcmI 2157 CCAGGTTCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2337 CCAGCCTGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AluI AlwI ApoI Asp700I
Asp718I AspHI AvaII BamHI BanI
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI