################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccIII AclI AgeI Aor51HI ApaI AscI AvaI AvrII BbeI BlnI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NotI NruI NspV PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2271 GACGTC GACGT^C AflII 8459 CTTAAG C^TTAAG Alw44I 4929 GTGCAC G^TGCAC ApaLI 4929 GTGCAC G^TGCAC AviII 587 TGCGCA TGC^GCA BbsI 240 GAAGAC GAAGAC(2/6) BcgI 2853 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 7131 GGATAC GTATCC(6/5) BclI 1617 TGATCA T^GATCA BfrI 8459 CTTAAG C^TTAAG BglI 5620 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3197 AGATCT A^GATCT BlpI 3182 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3182 GCTCAGC GC^T[ACGT]AGC BpuAI 240 GAAGAC GAAGAC(2/6) BsaBI 1276 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 8106 ACATGT A^CATGT BsrFI 65 ACCGGC [GA]^CCGG[CT] CelII 3182 GCTCAGC GC^T[ACGT]AGC Cfr10I 65 ACCGGC [GA]^CCGG[CT] DraII 6029 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 8007 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4186 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 6029 AGGACCC [GA]G^G[ACGT]CC[CT] FauI 7 CCCGC CCCGC(4/6) FbaI 1617 TGATCA T^GATCA FspI 587 TGCGCA TGC^GCA HaeII 5479 AGCGCC [GA]GCGC^[CT] HindIII 8636 AAGCTT A^AGCTT MamI 1276 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 380 CAATTG C^AATTG MunI 380 CAATTG C^AATTG NcoI 2019 CCATGG C^CATGG NheI 8138 GCTAGC G^CTAGC PacI 5487 TTAATTAA TTAAT^TAA PflMI 3083 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 6029 AGGACCC [GA]G^G[AT]CC[CT] PshAI 8162 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 375 GAGCTC GAGCT^C SbfI 5533 CCTGCAGG CCTGCA^GG SexAI 2156 ACCAGGT A^CC[AT]GGT Sse8387I 5533 CCTGCAGG CCTGCA^GG StuI 1069 AGGCCT AGG^CCT Van91I 3083 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 2271 GACGTC G[GA]^CG[CT]C 7537 GACGCC AflIII 4933 ACACGT A^C[GA][CT]GT 8106 ACATGT AhdI 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6031 GACCCTTAGTC AlwNI 1538 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3422 CAGATGCTG AocI 4639 CCTAAGG CC^T[ACGT]AGG 5768 CCTCAGG AseI 6282 ATTAAT AT^TAAT 8156 ATTAAT AsnI 6282 ATTAAT AT^TAAT 8156 ATTAAT AspEI 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6031 GACCCTTAGTC AspI 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4211 GACAGCGTC BalI 2952 TGGCCA TGG^CCA 5004 TGGCCA BanII 3 GAGCCC G[GA]GC[CT]^C 375 GAGCTC BbrPI 4934 CACGTG CAC^GTG 8323 CACGTG BbvCI 2839 CCTCAGC CCTCAGC(-5/-2) 6291 GCTGAGG BcnI 28 CCGGG CC^[GC]GG 85 CCCGG BsaAI 4934 CACGTG [CT]AC^GT[GA] 8323 CACGTG BsaHI 2271 GACGTC G[GA]^CG[CT]C 7537 GACGCC BsmI 3302 GAATGC GAATGC(1/-1) 5841 GAATGC BssSI 1318 CACGAG CACGAG(-5/-1) 3002 CACGAG Bsu36I 4639 CCTAAGG CC^T[ACGT]AGG 5768 CCTCAGG DsaI 2019 CCATGG C^C[GA][CT]GG 2748 CCACGG Eam1105I 4323 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6031 GACCCTTAGTC Eco81I 4639 CCTAAGG CC^T[ACGT]AGG 5768 CCTCAGG EcoNI 501 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6021 CCTTAGAAAGG EcoT22I 5197 ATGCAT ATGCA^T 7071 ATGCAT Hin1I 2271 GACGTC G[GA]^CG[CT]C 7537 GACGCC MluNI 2952 TGGCCA TGG^CCA 5004 TGGCCA MscI 2952 TGGCCA TGG^CCA 5004 TGGCCA NciI 28 CCGGG CC^[GC]GG 85 CCCGG NsiI 5197 ATGCAT ATGCA^T 7071 ATGCAT PflFI 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4211 GACAGCGTC PmaCI 4934 CACGTG CAC^GTG 8323 CACGTG PmlI 4934 CACGTG CAC^GTG 8323 CACGTG PshBI 6282 ATTAAT AT^TAAT 8156 ATTAAT PstI 2342 CTGCAG CTGCA^G 5534 CTGCAG SapI 570 GAAGAGC GCTCTTC(1/4) 3214 GAAGAGC Tth111I 2258 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4211 GACAGCGTC XcmI 2157 CCAGGTTCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2337 CCAGCCTGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I Asp718I AspHI AvaII BamHI BanI BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI