##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AscI AseI AsnI AviII AvrII
BamHI BbeI BbvCI BclI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI EagI EarI EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI TaqI TthHB8I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 264 GGTACC G^GTACC
AccI 2155 GTCTAC GT^[AC][GT]AC
AccII 1070 CGCG CG^CG
AclI 874 AACGTT AA^CGTT
AflIII 1325 ACATGT A^C[GA][CT]GT
AhdI 1860 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1433 GTGCAC G^TGCAC
AocI 2149 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1546 AGCGCT AGC^GCT
ApaI 1593 GGGCCC GGGCC^C
ApaLI 1433 GTGCAC G^TGCAC
Asp718I 264 GGTACC G^GTACC
AspEI 1860 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 387 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 915 CACGTG CAC^GTG
BbsI 2212 GAAGAC GAAGAC(2/6)
BciVI 677 GTATCC GTATCC(6/5)
BpuAI 2212 GAAGAC GAAGAC(2/6)
BsaAI 915 CACGTG [CT]AC^GT[GA]
BsaI 1462 GAGACC GGTCTC(1/5)
BsiEI 1072 CGATCG CG[GA][CT]^CG
BsmI 216 GCATTC GAATGC(1/-1)
BspHI 1961 TCATGA T^CATGA
BspLU11I 1325 ACATGT A^CATGT
BsrDI 629 GCAATG GCAATG(2/0)
BssSI 1738 CTCGTG CACGAG(-5/-1)
BstAPI 78 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1070 CGCG CG^CG
Bsu36I 2149 CCTTAGG CC^T[ACGT]AGG
DrdI 703 GACCCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 667 CCATGG C^C[GA][CT]GG
Eam1105I 1860 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1546 AGCGCT AGC^GCT
Eco81I 2149 CCTTAGG CC^T[ACGT]AGG
EcoRI 649 GAATTC G^AATTC
HincII 1197 GTCAAC GT[CT]^[GA]AC
HindII 1197 GTCAAC GT[CT]^[GA]AC
KpnI 264 GGTACC GGTAC^C
MvnI 1070 CGCG CG^CG
NaeI 1576 GCCGGC GCC^GGC
NcoI 667 CCATGG C^CATGG
NgoMIV 1576 GCCGGC G^CCGGC
PflFI 387 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 915 CACGTG CAC^GTG
PmlI 915 CACGTG CAC^GTG
PpuMI 561 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 874 AACGTT AA^CGTT
PvuI 1072 CGATCG CGAT^CG
PvuII 139 CAGCTG CAG^CTG
RcaI 1961 TCATGA T^CATGA
SfaNI 1045 GCATC GCATC(5/9)
SphI 1217 GCATGC GCATG^C
SspI 685 AATATT AAT^ATT
SwaI 2008 ATTTAAAT ATTT^AAAT
Tth111I 387 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 658 CCAGGAGTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 136 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1453 CAGTCCCTG
Asp700I 101 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1503 GAACCTTTTC
BalI 440 TGGCCA TGG^CCA
552 TGGCCA
BanI 264 GGTACC G^G[CT][GA]CC
835 GGTGCC
BcgI 1211 CGATCTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1066 GCACCGCGATCG
BcnI 1103 CCCGG CC^[GC]GG
1523 CCGGG
BlpI 6 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
BsgI 1316 CTGCAC GTGCAG(16/14)
1321 CTGCAC
BsmAI 1462 GAGAC GTCTC(1/5)
393 GTCTC
BsrFI 1041 ACCGGC [GA]^CCGG[CT]
1576 GCCGGC
BstEII 581 GGTTACC G^GT[ACGT]ACC
1681 GGTGACC
BstPI 581 GGTTACC G^GT[ACGT]ACC
1681 GGTGACC
BstYI 927 AGATCC [GA]^GATC[CT]
1641 AGATCC
CelII 6 GCTGAGC GC^T[ACGT]AGC
1402 GCTCAGC
Cfr10I 1041 ACCGGC [GA]^CCGG[CT]
1576 GCCGGC
EcoNI 1254 CCTAACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1438 CCTGCCACAGG
EcoO65I 581 GGTTACC G^GT[ACGT]ACC
1681 GGTGACC
FauI 1010 CCCGC CCCGC(4/6)
780 GCGGG
HaeII 472 GGCGCT [GA]GCGC^[CT]
1546 AGCGCT
MaeII 875 ACGT A^CGT
916 ACGT
MflI 927 AGATCC [GA]^GATC[CT]
1641 AGATCC
MluNI 440 TGGCCA TGG^CCA
552 TGGCCA
MscI 440 TGGCCA TGG^CCA
552 TGGCCA
MslI 1324 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1326 CATGTGTGTG
MspA1I 139 CAGCTG C[AC]G^C[GT]G
1003 CCGCTG
NciI 1103 CCCGG CC^[GC]GG
1523 CCGGG
PstI 58 CTGCAG CTGCA^G
1418 CTGCAG
SexAI 831 ACCTGGT A^CC[AT]GGT
1677 ACCTGGT
SmlI 110 CTCAAG C^T[CT][GA]AG
1397 CTCAAG
XhoII 927 AGATCC [GA]^GATC[CT]
1641 AGATCC
XmnI 101 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1503 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I