##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
Acc65I AclI AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbvCI BcgI
BlnI BsaBI BsrBI BssHII CpoI
EagI Eam1105I EclXI Eco52I EcoRV
FseI FspI HpaI KpnI MamI
MluI NaeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RsrII SalI SbfI
SfiI SgrAI SnaBI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
BbeI 4175 GGCGCC GGCGC^C
BsiWI 3261 CGTACG C^GTACG
BstBI 5843 TTCGAA TT^CGAA
BstEII 6025 GGTGACC G^GT[ACGT]ACC
BstPI 6025 GGTGACC G^GT[ACGT]ACC
EcoO65I 6025 GGTGACC G^GT[ACGT]ACC
EcoT22I 4568 ATGCAT ATGCA^T
KasI 4175 GGCGCC G^GCGCC
KspI 2589 CCGCGG CCGC^GG
NarI 4175 GGCGCC GG^CGCC
NheI 6400 GCTAGC G^CTAGC
NsiI 4568 ATGCAT ATGCA^T
NspV 5843 TTCGAA TT^CGAA
PvuI 612 CGATCG CGAT^CG
SacII 2589 CCGCGG CCGC^GG
SapI 4306 GAAGAGC GCTCTTC(1/4)
ScaI 3366 AGTACT AGT^ACT
SexAI 2554 ACCTGGT A^CC[AT]GGT
SfoI 4175 GGCGCC GGC^GCC
SfuI 5843 TTCGAA TT^CGAA
XbaI 2245 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 2199 TCCGGA T^CCGGA
4971 TCCGGA
AflII 3050 CTTAAG C^TTAAG
4858 CTTAAG
AocI 754 CCTGAGG CC^T[ACGT]AGG
3024 CCTCAGG
Aor51HI 4702 AGCGCT AGC^GCT
5610 AGCGCT
BclI 4756 TGATCA T^GATCA
6342 TGATCA
BfrI 3050 CTTAAG C^TTAAG
4858 CTTAAG
BlpI 3254 GCTGAGC GC^T[ACGT]AGC
3407 GCTCAGC
Bpu1102I 3254 GCTGAGC GC^T[ACGT]AGC
3407 GCTCAGC
BsaI 1005 GGTCTC GGTCTC(1/5)
4863 GGTCTC
BseAI 2199 TCCGGA T^CCGGA
4971 TCCGGA
BsmBI 2821 CGTCTC CGTCTC(1/5)
6530 CGTCTC
BsmI 2020 GAATGC GAATGC(1/-1)
2058 GAATGC
BspEI 2199 TCCGGA T^CCGGA
4971 TCCGGA
BspLU11I 534 ACATGT A^CATGT
4911 ACATGT
BsrFI 350 GCCGGT [GA]^CCGG[CT]
2142 ACCGGC
Bst1107I 4201 GTATAC GTA^TAC
7825 GTATAC
BstZ17I 4201 GTATAC GTA^TAC
7825 GTATAC
Bsu36I 754 CCTGAGG CC^T[ACGT]AGG
3024 CCTCAGG
CelII 3254 GCTGAGC GC^T[ACGT]AGC
3407 GCTCAGC
Cfr10I 350 GCCGGT [GA]^CCGG[CT]
2142 ACCGGC
DraIII 829 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3476 CACGCTGTG
DrdI 1075 GACGATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6732 GACCAGCTCGTC
Eco47III 4702 AGCGCT AGC^GCT
5610 AGCGCT
Eco81I 754 CCTGAGG CC^T[ACGT]AGG
3024 CCTCAGG
EcoRI 2618 GAATTC G^AATTC
3765 GAATTC
FbaI 4756 TGATCA T^GATCA
6342 TGATCA
MfeI 583 CAATTG C^AATTG
2256 CAATTG
MroI 2199 TCCGGA T^CCGGA
4971 TCCGGA
MunI 583 CAATTG C^AATTG
2256 CAATTG
NcoI 3402 CCATGG C^CATGG
6856 CCATGG
NdeI 3231 CATATG CA^TATG
6405 CATATG
PflMI 3826 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4528 CCAGTACATGG
SmaI 3656 CCCGGG CCC^GGG
4066 CCCGGG
SpeI 3037 ACTAGT A^CTAGT
7485 ACTAGT
StuI 4980 AGGCCT AGG^CCT
7594 AGGCCT
Van91I 3826 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4528 CCAGTACATGG
XmaCI 3656 CCCGGG C^CCGGG
4066 CCCGGG
XmaI 3656 CCCGGG C^CCGGG
4066 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
ClaI DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI