################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## Acc65I AclI AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbvCI BcgI BlnI BsaBI BsrBI BssHII CpoI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI HpaI KpnI MamI MluI NaeI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I RsrII SalI SbfI SfiI SgrAI SnaBI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### BbeI 4175 GGCGCC GGCGC^C BsiWI 3261 CGTACG C^GTACG BstBI 5843 TTCGAA TT^CGAA BstEII 6025 GGTGACC G^GT[ACGT]ACC BstPI 6025 GGTGACC G^GT[ACGT]ACC EcoO65I 6025 GGTGACC G^GT[ACGT]ACC EcoT22I 4568 ATGCAT ATGCA^T KasI 4175 GGCGCC G^GCGCC KspI 2589 CCGCGG CCGC^GG NarI 4175 GGCGCC GG^CGCC NheI 6400 GCTAGC G^CTAGC NsiI 4568 ATGCAT ATGCA^T NspV 5843 TTCGAA TT^CGAA PvuI 612 CGATCG CGAT^CG SacII 2589 CCGCGG CCGC^GG SapI 4306 GAAGAGC GCTCTTC(1/4) ScaI 3366 AGTACT AGT^ACT SexAI 2554 ACCTGGT A^CC[AT]GGT SfoI 4175 GGCGCC GGC^GCC SfuI 5843 TTCGAA TT^CGAA XbaI 2245 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccIII 2199 TCCGGA T^CCGGA 4971 TCCGGA AflII 3050 CTTAAG C^TTAAG 4858 CTTAAG AocI 754 CCTGAGG CC^T[ACGT]AGG 3024 CCTCAGG Aor51HI 4702 AGCGCT AGC^GCT 5610 AGCGCT BclI 4756 TGATCA T^GATCA 6342 TGATCA BfrI 3050 CTTAAG C^TTAAG 4858 CTTAAG BlpI 3254 GCTGAGC GC^T[ACGT]AGC 3407 GCTCAGC Bpu1102I 3254 GCTGAGC GC^T[ACGT]AGC 3407 GCTCAGC BsaI 1005 GGTCTC GGTCTC(1/5) 4863 GGTCTC BseAI 2199 TCCGGA T^CCGGA 4971 TCCGGA BsmBI 2821 CGTCTC CGTCTC(1/5) 6530 CGTCTC BsmI 2020 GAATGC GAATGC(1/-1) 2058 GAATGC BspEI 2199 TCCGGA T^CCGGA 4971 TCCGGA BspLU11I 534 ACATGT A^CATGT 4911 ACATGT BsrFI 350 GCCGGT [GA]^CCGG[CT] 2142 ACCGGC Bst1107I 4201 GTATAC GTA^TAC 7825 GTATAC BstZ17I 4201 GTATAC GTA^TAC 7825 GTATAC Bsu36I 754 CCTGAGG CC^T[ACGT]AGG 3024 CCTCAGG CelII 3254 GCTGAGC GC^T[ACGT]AGC 3407 GCTCAGC Cfr10I 350 GCCGGT [GA]^CCGG[CT] 2142 ACCGGC DraIII 829 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 3476 CACGCTGTG DrdI 1075 GACGATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6732 GACCAGCTCGTC Eco47III 4702 AGCGCT AGC^GCT 5610 AGCGCT Eco81I 754 CCTGAGG CC^T[ACGT]AGG 3024 CCTCAGG EcoRI 2618 GAATTC G^AATTC 3765 GAATTC FbaI 4756 TGATCA T^GATCA 6342 TGATCA MfeI 583 CAATTG C^AATTG 2256 CAATTG MroI 2199 TCCGGA T^CCGGA 4971 TCCGGA MunI 583 CAATTG C^AATTG 2256 CAATTG NcoI 3402 CCATGG C^CATGG 6856 CCATGG NdeI 3231 CATATG CA^TATG 6405 CATATG PflMI 3826 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4528 CCAGTACATGG SmaI 3656 CCCGGG CCC^GGG 4066 CCCGGG SpeI 3037 ACTAGT A^CTAGT 7485 ACTAGT StuI 4980 AGGCCT AGG^CCT 7594 AGGCCT Van91I 3826 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4528 CCAGTACATGG XmaCI 3656 CCCGGG C^CCGGG 4066 CCCGGG XmaI 3656 CCCGGG C^CCGGG 4066 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AatII AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I ClaI DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI