################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BpuAI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2057 GTCGAC GT^[AC][GT]AC AccIII 770 TCCGGA T^CCGGA AlwNI 2083 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 197 ATTAAT AT^TAAT AsnI 197 ATTAAT AT^TAAT AvaI 440 CTCGGG C^[CT]CG[GA]G AvrII 606 CCTAGG C^CTAGG BamHI 4 GGATCC G^GATCC BanI 1131 GGTGCC G^G[CT][GA]CC BciVI 1444 GGATAC GTATCC(6/5) BglII 1232 AGATCT A^GATCT BlnI 606 CCTAGG C^CTAGG BsaWI 770 TCCGGA [AT]^CCGG[AT] BseAI 770 TCCGGA T^CCGGA BseRI 186 GAGGAG GAGGAG(10/8) BsoBI 440 CTCGGG C^[CT]CG[GA]G BspDI 2004 ATCGAT AT^CGAT BspEI 770 TCCGGA T^CCGGA BspLU11I 1833 ACATGT A^CATGT BstBI 895 TTCGAA TT^CGAA CfoI 1082 GCGC GCG^C ClaI 2004 ATCGAT AT^CGAT DraI 1790 TTTAAA TTT^AAA DrdI 1644 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1482 GATATC GAT^ATC HapII 771 CCGG C^CGG HhaI 1082 GCGC GCG^C HinP1I 1082 GCGC G^CGC HpaII 771 CCGG C^CGG MroI 770 TCCGGA T^CCGGA MspI 771 CCGG C^CGG NcoI 2299 CCATGG C^CATGG NspV 895 TTCGAA TT^CGAA PleI 2471 GACTC GAGTC(4/5) PshAI 957 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 197 ATTAAT AT^TAAT PstI 1639 CTGCAG CTGCA^G SacI 1014 GAGCTC GAGCT^C SalI 2057 GTCGAC G^TCGAC SapI 654 GCTCTTC GCTCTTC(1/4) SfcI 1639 CTGCAG C^T[GA][CT]AG SfuI 895 TTCGAA TT^CGAA SmlI 1154 CTTGAG C^T[CT][GA]AG SpeI 1364 ACTAGT A^CTAGT StuI 1263 AGGCCT AGG^CCT XbaI 1436 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 475 ACACGT A^C[GA][CT]GT 1833 ACATGT ApaI 619 GGGCCC GGGCC^C 1988 GGGCCC Asp700I 839 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1069 GAATCCCTTC BalI 928 TGGCCA TGG^CCA 2161 TGGCCA BsaI 498 GAGACC GGTCTC(1/5) 2013 GAGACC BsmFI 2290 GGGAC GGGAC(10/14) 1697 GTCCC BsmI 1340 GAATGC GAATGC(1/-1) 406 GCATTC Bsp1407I 1828 TGTACA T^GTACA 2462 TGTACA BspMI 696 ACCTGC ACCTGC(4/8) 2311 ACCTGC BsrGI 1828 TGTACA T^GTACA 2462 TGTACA BstYI 4 GGATCC [GA]^GATC[CT] 1232 AGATCT Cac8I 1533 GCTTGC GC[ACGT]^[ACGT]GC 2321 GCCTGC EaeI 928 TGGCCA [CT]^GGCC[GA] 2161 TGGCCA EarI 655 CTCTTC CTCTTC(1/4) 1416 CTCTTC Ksp632I 655 CTCTTC CTCTTC(1/4) 1416 CTCTTC MflI 4 GGATCC [GA]^GATC[CT] 1232 AGATCT MluNI 928 TGGCCA TGG^CCA 2161 TGGCCA MscI 928 TGGCCA TGG^CCA 2161 TGGCCA PpuMI 282 AGGACCC [GA]G^G[AT]CC[CT] 602 AGGTCCT PvuII 1496 CAGCTG CAG^CTG 1669 CAGCTG SspBI 1828 TGTACA T^GTACA 2462 TGTACA Tsp45I 1361 GTCAC ^GT[GC]AC 1653 GTCAC XcmI 1998 CCAGTGATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2164 CCATCACACTCATGG XhoII 4 GGATCC [GA]^GATC[CT] 1232 AGATCT XmnI 839 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1069 GAATCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspHI BsrI BssKI BstF5I BstNI CelII Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I