##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BpuAI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2057 GTCGAC GT^[AC][GT]AC
AccIII 770 TCCGGA T^CCGGA
AlwNI 2083 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 197 ATTAAT AT^TAAT
AsnI 197 ATTAAT AT^TAAT
AvaI 440 CTCGGG C^[CT]CG[GA]G
AvrII 606 CCTAGG C^CTAGG
BamHI 4 GGATCC G^GATCC
BanI 1131 GGTGCC G^G[CT][GA]CC
BciVI 1444 GGATAC GTATCC(6/5)
BglII 1232 AGATCT A^GATCT
BlnI 606 CCTAGG C^CTAGG
BsaWI 770 TCCGGA [AT]^CCGG[AT]
BseAI 770 TCCGGA T^CCGGA
BseRI 186 GAGGAG GAGGAG(10/8)
BsoBI 440 CTCGGG C^[CT]CG[GA]G
BspDI 2004 ATCGAT AT^CGAT
BspEI 770 TCCGGA T^CCGGA
BspLU11I 1833 ACATGT A^CATGT
BstBI 895 TTCGAA TT^CGAA
CfoI 1082 GCGC GCG^C
ClaI 2004 ATCGAT AT^CGAT
DraI 1790 TTTAAA TTT^AAA
DrdI 1644 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1482 GATATC GAT^ATC
HapII 771 CCGG C^CGG
HhaI 1082 GCGC GCG^C
HinP1I 1082 GCGC G^CGC
HpaII 771 CCGG C^CGG
MroI 770 TCCGGA T^CCGGA
MspI 771 CCGG C^CGG
NcoI 2299 CCATGG C^CATGG
NspV 895 TTCGAA TT^CGAA
PleI 2471 GACTC GAGTC(4/5)
PshAI 957 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 197 ATTAAT AT^TAAT
PstI 1639 CTGCAG CTGCA^G
SacI 1014 GAGCTC GAGCT^C
SalI 2057 GTCGAC G^TCGAC
SapI 654 GCTCTTC GCTCTTC(1/4)
SfcI 1639 CTGCAG C^T[GA][CT]AG
SfuI 895 TTCGAA TT^CGAA
SmlI 1154 CTTGAG C^T[CT][GA]AG
SpeI 1364 ACTAGT A^CTAGT
StuI 1263 AGGCCT AGG^CCT
XbaI 1436 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 475 ACACGT A^C[GA][CT]GT
1833 ACATGT
ApaI 619 GGGCCC GGGCC^C
1988 GGGCCC
Asp700I 839 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAATCCCTTC
BalI 928 TGGCCA TGG^CCA
2161 TGGCCA
BsaI 498 GAGACC GGTCTC(1/5)
2013 GAGACC
BsmFI 2290 GGGAC GGGAC(10/14)
1697 GTCCC
BsmI 1340 GAATGC GAATGC(1/-1)
406 GCATTC
Bsp1407I 1828 TGTACA T^GTACA
2462 TGTACA
BspMI 696 ACCTGC ACCTGC(4/8)
2311 ACCTGC
BsrGI 1828 TGTACA T^GTACA
2462 TGTACA
BstYI 4 GGATCC [GA]^GATC[CT]
1232 AGATCT
Cac8I 1533 GCTTGC GC[ACGT]^[ACGT]GC
2321 GCCTGC
EaeI 928 TGGCCA [CT]^GGCC[GA]
2161 TGGCCA
EarI 655 CTCTTC CTCTTC(1/4)
1416 CTCTTC
Ksp632I 655 CTCTTC CTCTTC(1/4)
1416 CTCTTC
MflI 4 GGATCC [GA]^GATC[CT]
1232 AGATCT
MluNI 928 TGGCCA TGG^CCA
2161 TGGCCA
MscI 928 TGGCCA TGG^CCA
2161 TGGCCA
PpuMI 282 AGGACCC [GA]G^G[AT]CC[CT]
602 AGGTCCT
PvuII 1496 CAGCTG CAG^CTG
1669 CAGCTG
SspBI 1828 TGTACA T^GTACA
2462 TGTACA
Tsp45I 1361 GTCAC ^GT[GC]AC
1653 GTCAC
XcmI 1998 CCAGTGATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2164 CCATCACACTCATGG
XhoII 4 GGATCC [GA]^GATC[CT]
1232 AGATCT
XmnI 839 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1069 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI CelII Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I