##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BpuAI       BsaAI       BsaBI       BsaHI       
BsgI        BsiEI       BsiWI       BsmBI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspI        SwaI        
Tth111I     Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         2057  GTCGAC               GT^[AC][GT]AC
AccIII        770  TCCGGA               T^CCGGA
AlwNI        2083  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          197  ATTAAT               AT^TAAT
AsnI          197  ATTAAT               AT^TAAT
AvaI          440  CTCGGG               C^[CT]CG[GA]G
AvrII         606  CCTAGG               C^CTAGG
BamHI           4  GGATCC               G^GATCC
BanI         1131  GGTGCC               G^G[CT][GA]CC
BciVI        1444  GGATAC               GTATCC(6/5)
BglII        1232  AGATCT               A^GATCT
BlnI          606  CCTAGG               C^CTAGG
BsaWI         770  TCCGGA               [AT]^CCGG[AT]
BseAI         770  TCCGGA               T^CCGGA
BseRI         186  GAGGAG               GAGGAG(10/8)
BsoBI         440  CTCGGG               C^[CT]CG[GA]G
BspDI        2004  ATCGAT               AT^CGAT
BspEI         770  TCCGGA               T^CCGGA
BspLU11I     1833  ACATGT               A^CATGT
BstBI         895  TTCGAA               TT^CGAA
CfoI         1082  GCGC                 GCG^C
ClaI         2004  ATCGAT               AT^CGAT
DraI         1790  TTTAAA               TTT^AAA
DrdI         1644  GACCTCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1482  GATATC               GAT^ATC
HapII         771  CCGG                 C^CGG
HhaI         1082  GCGC                 GCG^C
HinP1I       1082  GCGC                 G^CGC
HpaII         771  CCGG                 C^CGG
MroI          770  TCCGGA               T^CCGGA
MspI          771  CCGG                 C^CGG
NcoI         2299  CCATGG               C^CATGG
NspV          895  TTCGAA               TT^CGAA
PleI         2471  GACTC                GAGTC(4/5)
PshAI         957  GACAATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         197  ATTAAT               AT^TAAT
PstI         1639  CTGCAG               CTGCA^G
SacI         1014  GAGCTC               GAGCT^C
SalI         2057  GTCGAC               G^TCGAC
SapI          654  GCTCTTC              GCTCTTC(1/4)
SfcI         1639  CTGCAG               C^T[GA][CT]AG
SfuI          895  TTCGAA               TT^CGAA
SmlI         1154  CTTGAG               C^T[CT][GA]AG
SpeI         1364  ACTAGT               A^CTAGT
StuI         1263  AGGCCT               AGG^CCT
XbaI         1436  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        475  ACACGT               A^C[GA][CT]GT
             1833  ACATGT              
ApaI          619  GGGCCC               GGGCC^C
             1988  GGGCCC              
Asp700I       839  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1069  GAATCCCTTC          
BalI          928  TGGCCA               TGG^CCA
             2161  TGGCCA              
BsaI          498  GAGACC               GGTCTC(1/5)
             2013  GAGACC              
BsmFI        2290  GGGAC                GGGAC(10/14)
             1697  GTCCC               
BsmI         1340  GAATGC               GAATGC(1/-1)
              406  GCATTC              
Bsp1407I     1828  TGTACA               T^GTACA
             2462  TGTACA              
BspMI         696  ACCTGC               ACCTGC(4/8)
             2311  ACCTGC              
BsrGI        1828  TGTACA               T^GTACA
             2462  TGTACA              
BstYI           4  GGATCC               [GA]^GATC[CT]
             1232  AGATCT              
Cac8I        1533  GCTTGC               GC[ACGT]^[ACGT]GC
             2321  GCCTGC              
EaeI          928  TGGCCA               [CT]^GGCC[GA]
             2161  TGGCCA              
EarI          655  CTCTTC               CTCTTC(1/4)
             1416  CTCTTC              
Ksp632I       655  CTCTTC               CTCTTC(1/4)
             1416  CTCTTC              
MflI            4  GGATCC               [GA]^GATC[CT]
             1232  AGATCT              
MluNI         928  TGGCCA               TGG^CCA
             2161  TGGCCA              
MscI          928  TGGCCA               TGG^CCA
             2161  TGGCCA              
PpuMI         282  AGGACCC              [GA]G^G[AT]CC[CT]
              602  AGGTCCT             
PvuII        1496  CAGCTG               CAG^CTG
             1669  CAGCTG              
SspBI        1828  TGTACA               T^GTACA
             2462  TGTACA              
Tsp45I       1361  GTCAC                ^GT[GC]AC
             1653  GTCAC               
XcmI         1998  CCAGTGATCGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2164  CCATCACACTCATGG     
XhoII           4  GGATCC               [GA]^GATC[CT]
             1232  AGATCT              
XmnI          839  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1069  GAATCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbvI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BspHI       BsrI        BssKI       BstF5I      
BstNI       CelII       Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HincII      HindII      
HinfI       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I