##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AatII AclI AgeI ApaI AscI
AviII BsiEI BsiWI BspDI Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I FseI
FspI KspI MfeI MunI NotI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SacII ScaI SfiI
SgrAI SnaBI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 859 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 766 AGCGCT AGC^GCT
Asp700I 571 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 859 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3063 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5741 CCCGGG C^[CT]CG[GA]G
AvrII 5138 CCTAGG C^CTAGG
BbrPI 2738 CACGTG CAC^GTG
BbvCI 6209 GCTGAGG CCTCAGC(-5/-2)
BlnI 5138 CCTAGG C^CTAGG
BlpI 1851 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1851 GCTGAGC GC^T[ACGT]AGC
BsaAI 2738 CACGTG [CT]AC^GT[GA]
BsoBI 5741 CCCGGG C^[CT]CG[GA]G
BsrBI 115 CCGCTC CCGCTC(-3/-3)
BssHII 72 GCGCGC G^CGCGC
BstAPI 2815 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2308 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1851 GCTGAGC GC^T[ACGT]AGC
Eam1105I 859 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 766 AGCGCT AGC^GCT
EcoRI 2028 GAATTC G^AATTC
MluI 3431 ACGCGT A^CGCGT
NdeI 4464 CATATG CA^TATG
NruI 3189 TCGCGA TCG^CGA
PflFI 3063 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2738 CACGTG CAC^GTG
PmlI 2738 CACGTG CAC^GTG
PshAI 4243 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3446 GTCGAC G^TCGAC
SbfI 123 CCTGCAGG CCTGCA^GG
SexAI 2990 ACCAGGT A^CC[AT]GGT
SmaI 5741 CCCGGG CCC^GGG
SpeI 5360 ACTAGT A^CTAGT
Sse8387I 123 CCTGCAGG CCTGCA^GG
Tth111I 3063 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6940 TCTAGA T^CTAGA
XmaCI 5741 CCCGGG C^CCGGG
XmaI 5741 CCCGGG C^CCGGG
XmnI 571 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4309 GGTACC G^GTACC
7510 GGTACC
AccIII 52 TCCGGA T^CCGGA
1858 TCCGGA
AflII 3906 CTTAAG C^TTAAG
4707 CTTAAG
AlwNI 1797 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
4288 CAGTTTCTG
AseI 5679 ATTAAT AT^TAAT
7606 ATTAAT
AsnI 5679 ATTAAT AT^TAAT
7606 ATTAAT
Asp718I 4309 GGTACC G^GTACC
7510 GGTACC
BalI 1783 TGGCCA TGG^CCA
1987 TGGCCA
BamHI 3533 GGATCC G^GATCC
4415 GGATCC
BciVI 4112 GTATCC GTATCC(6/5)
4132 GTATCC
BfrI 3906 CTTAAG C^TTAAG
4707 CTTAAG
BglI 178 GCCAACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2964 GCCCCCTCGGC
BsaBI 3255 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4573 GATTTCAATC
BseAI 52 TCCGGA T^CCGGA
1858 TCCGGA
BsmFI 5435 GGGAC GGGAC(10/14)
5275 GTCCC
BspEI 52 TCCGGA T^CCGGA
1858 TCCGGA
BspHI 7595 TCATGA T^CATGA
7615 TCATGA
BspLU11I 6411 ACATGT A^CATGT
7895 ACATGT
BsrDI 447 GCAATG GCAATG(2/0)
2203 GCAATG
BssSI 714 CACGAG CACGAG(-5/-1)
3842 CTCGTG
BstBI 3304 TTCGAA TT^CGAA
5047 TTCGAA
EcoRV 819 GATATC GAT^ATC
2625 GATATC
EcoT22I 2519 ATGCAT ATGCA^T
4422 ATGCAT
HpaI 6251 GTTAAC GTT^AAC
7683 GTTAAC
KpnI 4309 GGTACC GGTAC^C
7510 GGTACC
MamI 3255 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4573 GATTTCAATC
MluNI 1783 TGGCCA TGG^CCA
1987 TGGCCA
MroI 52 TCCGGA T^CCGGA
1858 TCCGGA
MscI 1783 TGGCCA TGG^CCA
1987 TGGCCA
NaeI 1477 GCCGGC GCC^GGC
1821 GCCGGC
NgoMIV 1477 GCCGGC G^CCGGC
1821 GCCGGC
NheI 1487 GCTAGC G^CTAGC
3368 GCTAGC
NsiI 2519 ATGCAT ATGCA^T
4422 ATGCAT
NspV 3304 TTCGAA TT^CGAA
5047 TTCGAA
PpuMI 3076 AGGACCT [GA]G^G[AT]CC[CT]
3310 AGGACCC
PshBI 5679 ATTAAT AT^TAAT
7606 ATTAAT
RcaI 7595 TCATGA T^CATGA
7615 TCATGA
SapI 912 GAAGAGC GCTCTTC(1/4)
5402 GAAGAGC
SfuI 3304 TTCGAA TT^CGAA
5047 TTCGAA
StuI 2389 AGGCCT AGG^CCT
7926 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AocI ApaLI ApoI AspHI AvaII
BanI BanII BbeI BbsI BbvI
BcgI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII