################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## Acc65I AclI AflII AgeI AscI Asp718I BbeI BfrI BsiWI BssHII CpoI DrdI FseI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SgrAI SmaI SnaBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 3134 GACGTC GACGT^C AocI 2153 CCTGAGG CC^T[ACGT]AGG AseI 1880 ATTAAT AT^TAAT AsnI 1880 ATTAAT AT^TAAT AviII 5067 TGCGCA TGC^GCA AvrII 3862 CCTAGG C^CTAGG BbrPI 2678 CACGTG CAC^GTG BciVI 6429 GTATCC GTATCC(6/5) BglII 6878 AGATCT A^GATCT BlnI 3862 CCTAGG C^CTAGG BsaBI 4627 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 4255 CGGCCG CG[GA][CT]^CG Bsp1407I 7338 TGTACA T^GTACA BspDI 3500 ATCGAT AT^CGAT BspLU11I 7279 ACATGT A^CATGT BsrGI 7338 TGTACA T^GTACA BssSI 3940 CACGAG CACGAG(-5/-1) Bst1107I 5746 GTATAC GTA^TAC BstAPI 3229 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 6905 TTCGAA TT^CGAA BstEII 607 GGTCACC G^GT[ACGT]ACC BstPI 607 GGTCACC G^GT[ACGT]ACC BstZ17I 5746 GTATAC GTA^TAC Bsu36I 2153 CCTGAGG CC^T[ACGT]AGG ClaI 3500 ATCGAT AT^CGAT EagI 4255 CGGCCG C^GGCCG EclXI 4255 CGGCCG C^GGCCG Eco52I 4255 CGGCCG C^GGCCG Eco81I 2153 CCTGAGG CC^T[ACGT]AGG EcoO65I 607 GGTCACC G^GT[ACGT]ACC EcoRV 5555 GATATC GAT^ATC FspI 5067 TGCGCA TGC^GCA HindIII 7626 AAGCTT A^AGCTT HpaI 6039 GTTAAC GTT^AAC MamI 4627 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1299 CCATGG C^CATGG NdeI 8094 CATATG CA^TATG NspV 6905 TTCGAA TT^CGAA PmaCI 2678 CACGTG CAC^GTG PmlI 2678 CACGTG CAC^GTG PshBI 1880 ATTAAT AT^TAAT SfuI 6905 TTCGAA TT^CGAA SpeI 2734 ACTAGT A^CTAGT SspBI 7338 TGTACA T^GTACA SspI 6239 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AhdI 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1383 GACCCGGTGTC Aor51HI 1211 AGCGCT AGC^GCT 4671 AGCGCT ApaI 1733 GGGCCC GGGCC^C 3367 GGGCCC Asp700I 3835 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4165 GAAGGTATTC AspEI 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1383 GACCCGGTGTC BcgI 241 CGAGAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2254 CGACGAAGATGC BglI 324 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4049 GCCTGCACGGC BsrBI 660 GAGCGG CCGCTC(-3/-3) 1978 GAGCGG BstXI 2182 CCATCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2979 CCAAGGCTGTGG Eam1105I 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1383 GACCCGGTGTC Eco47III 1211 AGCGCT AGC^GCT 4671 AGCGCT PaeR7I 2397 CTCGAG C^TCGAG 3087 CTCGAG PvuII 2833 CAGCTG CAG^CTG 4307 CAGCTG SbfI 2309 CCTGCAGG CCTGCA^GG 7856 CCTGCAGG SexAI 4710 ACCTGGT A^CC[AT]GGT 7375 ACCTGGT Sse8387I 2309 CCTGCAGG CCTGCA^GG 7856 CCTGCAGG XcmI 4774 CCAAGTCAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5894 CCAGGCGTTCTGTGG XhoI 2397 CTCGAG C^TCGAG 3087 CTCGAG XmnI 3835 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4165 GAAGGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 152 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PpuMI PstI RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoII