##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I BbeI BfrI BsiWI BssHII
CpoI DrdI FseI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SfiI
SfoI SgrAI SmaI SnaBI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3134 GACGTC GACGT^C
AocI 2153 CCTGAGG CC^T[ACGT]AGG
AseI 1880 ATTAAT AT^TAAT
AsnI 1880 ATTAAT AT^TAAT
AviII 5067 TGCGCA TGC^GCA
AvrII 3862 CCTAGG C^CTAGG
BbrPI 2678 CACGTG CAC^GTG
BciVI 6429 GTATCC GTATCC(6/5)
BglII 6878 AGATCT A^GATCT
BlnI 3862 CCTAGG C^CTAGG
BsaBI 4627 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4255 CGGCCG CG[GA][CT]^CG
Bsp1407I 7338 TGTACA T^GTACA
BspDI 3500 ATCGAT AT^CGAT
BspLU11I 7279 ACATGT A^CATGT
BsrGI 7338 TGTACA T^GTACA
BssSI 3940 CACGAG CACGAG(-5/-1)
Bst1107I 5746 GTATAC GTA^TAC
BstAPI 3229 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 6905 TTCGAA TT^CGAA
BstEII 607 GGTCACC G^GT[ACGT]ACC
BstPI 607 GGTCACC G^GT[ACGT]ACC
BstZ17I 5746 GTATAC GTA^TAC
Bsu36I 2153 CCTGAGG CC^T[ACGT]AGG
ClaI 3500 ATCGAT AT^CGAT
EagI 4255 CGGCCG C^GGCCG
EclXI 4255 CGGCCG C^GGCCG
Eco52I 4255 CGGCCG C^GGCCG
Eco81I 2153 CCTGAGG CC^T[ACGT]AGG
EcoO65I 607 GGTCACC G^GT[ACGT]ACC
EcoRV 5555 GATATC GAT^ATC
FspI 5067 TGCGCA TGC^GCA
HindIII 7626 AAGCTT A^AGCTT
HpaI 6039 GTTAAC GTT^AAC
MamI 4627 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1299 CCATGG C^CATGG
NdeI 8094 CATATG CA^TATG
NspV 6905 TTCGAA TT^CGAA
PmaCI 2678 CACGTG CAC^GTG
PmlI 2678 CACGTG CAC^GTG
PshBI 1880 ATTAAT AT^TAAT
SfuI 6905 TTCGAA TT^CGAA
SpeI 2734 ACTAGT A^CTAGT
SspBI 7338 TGTACA T^GTACA
SspI 6239 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AhdI 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1383 GACCCGGTGTC
Aor51HI 1211 AGCGCT AGC^GCT
4671 AGCGCT
ApaI 1733 GGGCCC GGGCC^C
3367 GGGCCC
Asp700I 3835 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4165 GAAGGTATTC
AspEI 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1383 GACCCGGTGTC
BcgI 241 CGAGAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2254 CGACGAAGATGC
BglI 324 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4049 GCCTGCACGGC
BsrBI 660 GAGCGG CCGCTC(-3/-3)
1978 GAGCGG
BstXI 2182 CCATCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCAAGGCTGTGG
Eam1105I 1337 GACTTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1383 GACCCGGTGTC
Eco47III 1211 AGCGCT AGC^GCT
4671 AGCGCT
PaeR7I 2397 CTCGAG C^TCGAG
3087 CTCGAG
PvuII 2833 CAGCTG CAG^CTG
4307 CAGCTG
SbfI 2309 CCTGCAGG CCTGCA^GG
7856 CCTGCAGG
SexAI 4710 ACCTGGT A^CC[AT]GGT
7375 ACCTGGT
Sse8387I 2309 CCTGCAGG CCTGCA^GG
7856 CCTGCAGG
XcmI 4774 CCAAGTCAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5894 CCAGGCGTTCTGTGG
XhoI 2397 CTCGAG C^TCGAG
3087 CTCGAG
XmnI 3835 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4165 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PflMI PleI PpuMI
PstI RcaI RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoII