##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI BclI BsaBI BsiWI
Bsp1407I BspDI BspHI BsrGI Bst1107I
BstZ17I ClaI CpoI DraIII Eam1105I
FbaI FseI HpaI MamI MluI
NaeI NdeI NgoMIV NotI NruI
PacI PaeR7I PmeI PshBI PvuI
RcaI RsrII SbfI SfiI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 5197 GACGTC GACGT^C
AclI 1072 AACGTT AA^CGTT
AgeI 2146 ACCGGT A^CCGGT
Asp700I 474 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 536 TGCGCA TGC^GCA
AvrII 783 CCTAGG C^CTAGG
BbrPI 4564 CACGTG CAC^GTG
BlnI 783 CCTAGG C^CTAGG
BssHII 417 GCGCGC G^CGCGC
BssSI 7885 CACGAG CACGAG(-5/-1)
BstBI 6483 TTCGAA TT^CGAA
EcoRV 4054 GATATC GAT^ATC
FspI 536 TGCGCA TGC^GCA
KspI 386 CCGCGG CCGC^GG
MfeI 3061 CAATTG C^AATTG
MunI 3061 CAATTG C^AATTG
NspV 6483 TTCGAA TT^CGAA
PinAI 2146 ACCGGT A^CCGGT
PmaCI 4564 CACGTG CAC^GTG
PmlI 4564 CACGTG CAC^GTG
Psp1406I 1072 AACGTT AA^CGTT
SacII 386 CCGCGG CCGC^GG
ScaI 7212 AGTACT AGT^ACT
SfuI 6483 TTCGAA TT^CGAA
SgrAI 4738 CACCGGCG C[GA]^CCGG[CT]G
XbaI 2679 TCTAGA T^CTAGA
XmnI 474 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 3113 GGTACC G^GTACC
5053 GGTACC
AccI 4666 GTCGAC GT^[AC][GT]AC
6198 GTCGAC
Asp718I 3113 GGTACC G^GTACC
5053 GGTACC
BciVI 190 GGATAC GTATCC(6/5)
8295 GGATAC
BsaAI 4564 CACGTG [CT]AC^GT[GA]
7715 TACGTG
BsiEI 3488 CGGCCG CG[GA][CT]^CG
4824 CGGCCG
BsmBI 3484 GAGACG CGTCTC(1/5)
4684 GAGACG
BsmI 272 GAATGC GAATGC(1/-1)
711 GCATTC
BspLU11I 6132 ACATGT A^CATGT
6843 ACATGT
EagI 3488 CGGCCG C^GGCCG
4824 CGGCCG
EclXI 3488 CGGCCG C^GGCCG
4824 CGGCCG
Eco52I 3488 CGGCCG C^GGCCG
4824 CGGCCG
EcoRI 3552 GAATTC G^AATTC
3633 GAATTC
EcoT22I 1321 ATGCAT ATGCA^T
6527 ATGCAT
KpnI 3113 GGTACC GGTAC^C
5053 GGTACC
NcoI 4304 CCATGG C^CATGG
5088 CCATGG
NsiI 1321 ATGCAT ATGCA^T
6527 ATGCAT
PshAI 3067 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
8069 GACATAGGTC
SacI 1632 GAGCTC GAGCT^C
2763 GAGCTC
SalI 4666 GTCGAC G^TCGAC
6198 GTCGAC
SspI 2824 AATATT AAT^ATT
6711 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI Aor51HI ApaI ApoI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BfrI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NheI NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII