##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BlnI BsaHI BseAI BsiWI
BsmBI BspEI BsrBI BssHII BstAPI
BstUI CpoI Eam1105I Eco47III FseI
FspI HaeII Hin1I HpaI KasI
KspI MluI MroI MvnI NarI
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 1330 AACGTT AA^CGTT
AflII 1358 CTTAAG C^TTAAG
AocI 8505 CCTGAGG CC^T[ACGT]AGG
AseI 4033 ATTAAT AT^TAAT
AsnI 4033 ATTAAT AT^TAAT
BfrI 1358 CTTAAG C^TTAAG
BlpI 8015 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 8015 GCTGAGC GC^T[ACGT]AGC
BsaAI 3538 TACGTG [CT]AC^GT[GA]
BsaI 6789 GAGACC GGTCTC(1/5)
BsaWI 2537 ACCGGA [AT]^CCGG[AT]
BsiEI 1723 CGGCCG CG[GA][CT]^CG
BspDI 2196 ATCGAT AT^CGAT
BsrFI 1155 GCCGGC [GA]^CCGG[CT]
BssSI 7249 CACGAG CACGAG(-5/-1)
Bsu36I 8505 CCTGAGG CC^T[ACGT]AGG
CelII 8015 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1155 GCCGGC [GA]^CCGG[CT]
ClaI 2196 ATCGAT AT^CGAT
EagI 1723 CGGCCG C^GGCCG
EclXI 1723 CGGCCG C^GGCCG
Eco52I 1723 CGGCCG C^GGCCG
Eco81I 8505 CCTGAGG CC^T[ACGT]AGG
HgaI 1415 GCGTC GACGC(5/10)
HindIII 7183 AAGCTT A^AGCTT
NaeI 1155 GCCGGC GCC^GGC
NgoMIV 1155 GCCGGC G^CCGGC
PaeR7I 5223 CTCGAG C^TCGAG
PshAI 1580 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4033 ATTAAT AT^TAAT
Psp1406I 1330 AACGTT AA^CGTT
SexAI 6451 ACCTGGT A^CC[AT]GGT
SpeI 7234 ACTAGT A^CTAGT
StuI 2720 AGGCCT AGG^CCT
XbaI 8906 TCTAGA T^CTAGA
XhoI 5223 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 392 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2311 CAGGGACTG
AvaI 2912 CCCGAG C^[CT]CG[GA]G
5223 CTCGAG
BcgI 2490 CGAATACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
8169 CGATACTTGTGC
BclI 843 TGATCA T^GATCA
3502 TGATCA
BcnI 2569 CCGGG CC^[GC]GG
3650 CCCGG
BglI 6690 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6864 GCCAGCCAGGC
BglII 6981 AGATCT A^GATCT
7628 AGATCT
BsaBI 4169 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4228 GATCTACATC
BsoBI 2912 CCCGAG C^[CT]CG[GA]G
5223 CTCGAG
FbaI 843 TGATCA T^GATCA
3502 TGATCA
MamI 4169 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4228 GATCTACATC
NciI 2569 CCGGG CC^[GC]GG
3650 CCCGG
NdeI 975 CATATG CA^TATG
1779 CATATG
SacI 153 GAGCTC GAGCT^C
5270 GAGCTC
SapI 611 GAAGAGC GCTCTTC(1/4)
6180 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI Alw44I AlwI ApaLI
ApoI Asp700I Asp718I AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssKI Bst1107I
BstBI BstEII BstF5I BstNI BstPI
BstXI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI NspV PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI