##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Aor51HI AscI AviII BbeI BcgI
BglI BsaBI BsiEI BsiWI BsmBI
BsrBI BssHII Bst1107I BstZ17I CpoI
EagI EclXI Eco47III Eco52I FseI
FspI HpaI KasI MamI MluI
NaeI NarI NgoMIV NotI NruI
PacI PshAI PvuI RsrII SalI
SfiI SfoI SgrAI SmaI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3542 GACGTC GACGT^C
Acc65I 6715 GGTACC G^GTACC
AccIII 3554 TCCGGA T^CCGGA
AclI 5555 AACGTT AA^CGTT
AgeI 5059 ACCGGT A^CCGGT
AhdI 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4600 ATTAAT AT^TAAT
AsnI 4600 ATTAAT AT^TAAT
Asp718I 6715 GGTACC G^GTACC
AspEI 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 6715 GGTACC G^G[CT][GA]CC
BciVI 6161 GTATCC GTATCC(6/5)
BseAI 3554 TCCGGA T^CCGGA
BspDI 2033 ATCGAT AT^CGAT
BspEI 3554 TCCGGA T^CCGGA
BsrDI 3588 GCAATG GCAATG(2/0)
BsrFI 5059 ACCGGT [GA]^CCGG[CT]
BssSI 2554 CTCGTG CACGAG(-5/-1)
BstBI 2296 TTCGAA TT^CGAA
BstXI 1831 CCATGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 5059 ACCGGT [GA]^CCGG[CT]
ClaI 2033 ATCGAT AT^CGAT
Eam1105I 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2154 GATATC GAT^ATC
KpnI 6715 GGTACC GGTAC^C
KspI 5929 CCGCGG CCGC^GG
MroI 3554 TCCGGA T^CCGGA
NheI 7161 GCTAGC G^CTAGC
NspV 2296 TTCGAA TT^CGAA
PflFI 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5059 ACCGGT A^CCGGT
PshBI 4600 ATTAAT AT^TAAT
Psp1406I 5555 AACGTT AA^CGTT
SacII 5929 CCGCGG CCGC^GG
SbfI 2915 CCTGCAGG CCTGCA^GG
SfuI 2296 TTCGAA TT^CGAA
SnaBI 8948 TACGTA TAC^GTA
SphI 2278 GCATGC GCATG^C
Sse8387I 2915 CCTGCAGG CCTGCA^GG
Tth111I 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6560 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 282 GTAGAC GT^[AC][GT]AC
8504 GTAGAC
AcyI 3542 GACGTC G[GA]^CG[CT]C
5761 GACGCC
AflII 7565 CTTAAG C^TTAAG
8773 CTTAAG
AocI 3177 CCTGAGG CC^T[ACGT]AGG
4475 CCTTAGG
ApaI 8550 GGGCCC GGGCC^C
9156 GGGCCC
BamHI 5814 GGATCC G^GATCC
7079 GGATCC
BbrPI 397 CACGTG CAC^GTG
3507 CACGTG
BbvCI 728 CCTCAGC CCTCAGC(-5/-2)
6330 CCTCAGC
BcnI 1741 CCGGG CC^[GC]GG
8962 CCGGG
BfrI 7565 CTTAAG C^TTAAG
8773 CTTAAG
BglII 4000 AGATCT A^GATCT
4036 AGATCT
BsaHI 3542 GACGTC G[GA]^CG[CT]C
5761 GACGCC
BsgI 409 CTGCAC GTGCAG(16/14)
8103 CTGCAC
Bsu36I 3177 CCTGAGG CC^T[ACGT]AGG
4475 CCTTAGG
Eco81I 3177 CCTGAGG CC^T[ACGT]AGG
4475 CCTTAGG
HaeII 929 AGCGCC [GA]GCGC^[CT]
6384 AGCGCC
HgaI 5761 GACGC GACGC(5/10)
7304 GCGTC
Hin1I 3542 GACGTC G[GA]^CG[CT]C
5761 GACGCC
MfeI 2530 CAATTG C^AATTG
5574 CAATTG
MunI 2530 CAATTG C^AATTG
5574 CAATTG
NciI 1741 CCGGG CC^[GC]GG
8962 CCGGG
NdeI 6156 CATATG CA^TATG
7751 CATATG
PaeR7I 363 CTCGAG C^TCGAG
2663 CTCGAG
PmaCI 397 CACGTG CAC^GTG
3507 CACGTG
PmeI 3412 GTTTAAAC GTTT^AAAC
5558 GTTTAAAC
PmlI 397 CACGTG CAC^GTG
3507 CACGTG
SapI 1714 GAAGAGC GCTCTTC(1/4)
3570 GCTCTTC
ScaI 3978 AGTACT AGT^ACT
4460 AGTACT
SexAI 2186 ACCAGGT A^CC[AT]GGT
8371 ACCTGGT
SpeI 3024 ACTAGT A^CTAGT
7787 ACTAGT
SspI 2652 AATATT AAT^ATT
9207 AATATT
StuI 659 AGGCCT AGG^CCT
6320 AGGCCT
XhoI 363 CTCGAG C^TCGAG
2663 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BanII BbsI BbvI
BclI BfaI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI