################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## Aor51HI AscI AviII BbeI BcgI BglI BsaBI BsiEI BsiWI BsmBI BsrBI BssHII Bst1107I BstZ17I CpoI EagI EclXI Eco47III Eco52I FseI FspI HpaI KasI MamI MluI NaeI NarI NgoMIV NotI NruI PacI PshAI PvuI RsrII SalI SfiI SfoI SgrAI SmaI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 3542 GACGTC GACGT^C Acc65I 6715 GGTACC G^GTACC AccIII 3554 TCCGGA T^CCGGA AclI 5555 AACGTT AA^CGTT AgeI 5059 ACCGGT A^CCGGT AhdI 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 4600 ATTAAT AT^TAAT AsnI 4600 ATTAAT AT^TAAT Asp718I 6715 GGTACC G^GTACC AspEI 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 6715 GGTACC G^G[CT][GA]CC BciVI 6161 GTATCC GTATCC(6/5) BseAI 3554 TCCGGA T^CCGGA BspDI 2033 ATCGAT AT^CGAT BspEI 3554 TCCGGA T^CCGGA BsrDI 3588 GCAATG GCAATG(2/0) BsrFI 5059 ACCGGT [GA]^CCGG[CT] BssSI 2554 CTCGTG CACGAG(-5/-1) BstBI 2296 TTCGAA TT^CGAA BstXI 1831 CCATGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 5059 ACCGGT [GA]^CCGG[CT] ClaI 2033 ATCGAT AT^CGAT Eam1105I 8324 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 2154 GATATC GAT^ATC KpnI 6715 GGTACC GGTAC^C KspI 5929 CCGCGG CCGC^GG MroI 3554 TCCGGA T^CCGGA NheI 7161 GCTAGC G^CTAGC NspV 2296 TTCGAA TT^CGAA PflFI 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5059 ACCGGT A^CCGGT PshBI 4600 ATTAAT AT^TAAT Psp1406I 5555 AACGTT AA^CGTT SacII 5929 CCGCGG CCGC^GG SbfI 2915 CCTGCAGG CCTGCA^GG SfuI 2296 TTCGAA TT^CGAA SnaBI 8948 TACGTA TAC^GTA SphI 2278 GCATGC GCATG^C Sse8387I 2915 CCTGCAGG CCTGCA^GG Tth111I 1915 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 6560 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 282 GTAGAC GT^[AC][GT]AC 8504 GTAGAC AcyI 3542 GACGTC G[GA]^CG[CT]C 5761 GACGCC AflII 7565 CTTAAG C^TTAAG 8773 CTTAAG AocI 3177 CCTGAGG CC^T[ACGT]AGG 4475 CCTTAGG ApaI 8550 GGGCCC GGGCC^C 9156 GGGCCC BamHI 5814 GGATCC G^GATCC 7079 GGATCC BbrPI 397 CACGTG CAC^GTG 3507 CACGTG BbvCI 728 CCTCAGC CCTCAGC(-5/-2) 6330 CCTCAGC BcnI 1741 CCGGG CC^[GC]GG 8962 CCGGG BfrI 7565 CTTAAG C^TTAAG 8773 CTTAAG BglII 4000 AGATCT A^GATCT 4036 AGATCT BsaHI 3542 GACGTC G[GA]^CG[CT]C 5761 GACGCC BsgI 409 CTGCAC GTGCAG(16/14) 8103 CTGCAC Bsu36I 3177 CCTGAGG CC^T[ACGT]AGG 4475 CCTTAGG Eco81I 3177 CCTGAGG CC^T[ACGT]AGG 4475 CCTTAGG HaeII 929 AGCGCC [GA]GCGC^[CT] 6384 AGCGCC HgaI 5761 GACGC GACGC(5/10) 7304 GCGTC Hin1I 3542 GACGTC G[GA]^CG[CT]C 5761 GACGCC MfeI 2530 CAATTG C^AATTG 5574 CAATTG MunI 2530 CAATTG C^AATTG 5574 CAATTG NciI 1741 CCGGG CC^[GC]GG 8962 CCGGG NdeI 6156 CATATG CA^TATG 7751 CATATG PaeR7I 363 CTCGAG C^TCGAG 2663 CTCGAG PmaCI 397 CACGTG CAC^GTG 3507 CACGTG PmeI 3412 GTTTAAAC GTTT^AAAC 5558 GTTTAAAC PmlI 397 CACGTG CAC^GTG 3507 CACGTG SapI 1714 GAAGAGC GCTCTTC(1/4) 3570 GCTCTTC ScaI 3978 AGTACT AGT^ACT 4460 AGTACT SexAI 2186 ACCAGGT A^CC[AT]GGT 8371 ACCTGGT SpeI 3024 ACTAGT A^CTAGT 7787 ACTAGT SspI 2652 AATATT AAT^ATT 9207 AATATT StuI 659 AGGCCT AGG^CCT 6320 AGGCCT XhoI 363 CTCGAG C^TCGAG 2663 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BanII BbsI BbvI BclI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI