##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BciVI BlnI BsaAI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HincII
HindII HpaI KpnI KspI MluI
MroI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 497 GTCTAC GT^[AC][GT]AC
AflII 1634 CTTAAG C^TTAAG
AgeI 2206 ACCGGT A^CCGGT
Aor51HI 2258 AGCGCT AGC^GCT
Asp700I 2534 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2003 GGATCC G^GATCC
BanI 13 GGCGCC G^G[CT][GA]CC
BbeI 13 GGCGCC GGCGC^C
BbvCI 1527 GCTGAGG CCTCAGC(-5/-2)
BclI 1594 TGATCA T^GATCA
BfrI 1634 CTTAAG C^TTAAG
BglII 2357 AGATCT A^GATCT
BlpI 969 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 969 GCTCAGC GC^T[ACGT]AGC
BsaI 84 GAGACC GGTCTC(1/5)
BsaWI 2206 ACCGGT [AT]^CCGG[AT]
BsiEI 2159 CGACCG CG[GA][CT]^CG
BsmAI 84 GAGAC GTCTC(1/5)
BspHI 1501 TCATGA T^CATGA
BsrBI 1956 GAGCGG CCGCTC(-3/-3)
CelII 969 GCTCAGC GC^T[ACGT]AGC
DraI 1472 TTTAAA TTT^AAA
DrdI 1286 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1895 CCATGG C^C[GA][CT]GG
Eco47III 2258 AGCGCT AGC^GCT
FbaI 1594 TGATCA T^GATCA
HindIII 289 AAGCTT A^AGCTT
KasI 13 GGCGCC G^GCGCC
MfeI 1560 CAATTG C^AATTG
MunI 1560 CAATTG C^AATTG
NarI 13 GGCGCC GG^CGCC
NcoI 1895 CCATGG C^CATGG
NdeI 3187 CATATG CA^TATG
PinAI 2206 ACCGGT A^CCGGT
RcaI 1501 TCATGA T^CATGA
SacI 2112 GAGCTC GAGCT^C
SapI 1646 GAAGAGC GCTCTTC(1/4)
SfoI 13 GGCGCC GGC^GCC
SgrAI 2205 CACCGGTG C[GA]^CCGG[CT]G
SmaI 217 CCCGGG CCC^GGG
SphI 364 GCATGC GCATG^C
StuI 2293 AGGCCT AGG^CCT
XcmI 3075 CCACCAAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 217 CCCGGG C^CCGGG
XmaI 217 CCCGGG C^CCGGG
XmnI 2534 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
1221 GGCGTC
AflIII 2794 ACACGT A^C[GA][CT]GT
2807 ACACGT
Alw44I 2202 GTGCAC G^TGCAC
3173 GTGCAC
AocI 963 CCTCAGG CC^T[ACGT]AGG
2780 CCTAAGG
ApaLI 2202 GTGCAC G^TGCAC
3173 GTGCAC
BalI 1825 TGGCCA TGG^CCA
1888 TGGCCA
BsaBI 222 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1886 GATGGCCATC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
1221 GGCGTC
BsmI 311 GAATGC GAATGC(1/-1)
662 GAATGC
Bsu36I 963 CCTCAGG CC^T[ACGT]AGG
2780 CCTAAGG
EarI 1646 GAAGAG CTCTTC(1/4)
1679 GAAGAG
Eco81I 963 CCTCAGG CC^T[ACGT]AGG
2780 CCTAAGG
EcoNI 1675 CCTCGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2407 CCTGGGTTAGG
EcoT14I 410 CCTTGG C^C[AT][AT]GG
1895 CCATGG
HgaI 1588 GACGC GACGC(5/10)
1222 GCGTC
Hin1I 13 GGCGCC G[GA]^CG[CT]C
1221 GGCGTC
Ksp632I 1646 GAAGAG CTCTTC(1/4)
1679 GAAGAG
MamI 222 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1886 GATGGCCATC
MluNI 1825 TGGCCA TGG^CCA
1888 TGGCCA
MscI 1825 TGGCCA TGG^CCA
1888 TGGCCA
NspI 364 GCATGC [GA]CATG^[CT]
2786 GCATGT
StyI 410 CCTTGG C^C[AT][AT]GG
1895 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII