##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
Asp718I BbrPI BsiWI BssHII BstBI
CpoI Eco47III EcoT22I KpnI KspI
MfeI MluI MunI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SfuI SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 8535 AACGTT AA^CGTT
AcyI 6295 GGCGCC G[GA]^CG[CT]C
AseI 4918 ATTAAT AT^TAAT
AsnI 4918 ATTAAT AT^TAAT
AspI 1232 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 816 TGCGCA TGC^GCA
BbeI 6295 GGCGCC GGCGC^C
BglII 429 AGATCT A^GATCT
BsaAI 6221 CACGTA [CT]AC^GT[GA]
BsaHI 6295 GGCGCC G[GA]^CG[CT]C
BsiEI 8 CGGCCG CG[GA][CT]^CG
Bst1107I 2882 GTATAC GTA^TAC
BstAPI 4493 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2882 GTATAC GTA^TAC
DraI 3112 TTTAAA TTT^AAA
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
FseI 5 GGCCGGCC GGCCGG^CC
FspI 816 TGCGCA TGC^GCA
Hin1I 6295 GGCGCC G[GA]^CG[CT]C
HpaI 1488 GTTAAC GTT^AAC
KasI 6295 GGCGCC G^GCGCC
NarI 6295 GGCGCC GG^CGCC
NheI 1216 GCTAGC G^CTAGC
PaeR7I 4121 CTCGAG C^TCGAG
PflFI 1232 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4918 ATTAAT AT^TAAT
Psp1406I 8535 AACGTT AA^CGTT
ScaI 492 AGTACT AGT^ACT
SexAI 2143 ACCAGGT A^CC[AT]GGT
SfoI 6295 GGCGCC GGC^GCC
SpeI 2914 ACTAGT A^CTAGT
SspI 8940 AATATT AAT^ATT
Tth111I 1232 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3507 TCTAGA T^CTAGA
XhoI 4121 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2882 GTATAC GT^[AC][GT]AC
8551 GTCTAC
Alw44I 2210 GTGCAC G^TGCAC
8615 GTGCAC
ApaLI 2210 GTGCAC G^TGCAC
8615 GTGCAC
AvrII 4189 CCTAGG C^CTAGG
7054 CCTAGG
BamHI 460 GGATCC G^GATCC
1116 GGATCC
BbvCI 2921 GCTGAGG CCTCAGC(-5/-2)
5572 GCTGAGG
BcgI 4123 CGAGTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2081 GCAGGTATTTCG
BciVI 8728 GGATAC GTATCC(6/5)
4712 GTATCC
BclI 5789 TGATCA T^GATCA
7807 TGATCA
BlnI 4189 CCTAGG C^CTAGG
7054 CCTAGG
BspDI 638 ATCGAT AT^CGAT
5272 ATCGAT
BsrDI 1957 GCAATG GCAATG(2/0)
6535 GCAATG
BssSI 1148 CACGAG CACGAG(-5/-1)
5960 CACGAG
BstEII 6420 GGTCACC G^GT[ACGT]ACC
7239 GGTAACC
BstPI 6420 GGTCACC G^GT[ACGT]ACC
7239 GGTAACC
ClaI 638 ATCGAT AT^CGAT
5272 ATCGAT
DraIII 2054 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2763 CACTTGGTG
EcoO65I 6420 GGTCACC G^GT[ACGT]ACC
7239 GGTAACC
EcoRV 5818 GATATC GAT^ATC
8803 GATATC
FbaI 5789 TGATCA T^GATCA
7807 TGATCA
HgaI 1637 GACGC GACGC(5/10)
2472 GACGC
HindIII 979 AAGCTT A^AGCTT
5320 AAGCTT
NaeI 6 GCCGGC GCC^GGC
1573 GCCGGC
NdeI 3383 CATATG CA^TATG
4757 CATATG
NgoMIV 6 GCCGGC G^CCGGC
1573 GCCGGC
PflMI 7773 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8765 CCACCAACTGG
PshAI 7188 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7247 GACCACTGTC
SbfI 6147 CCTGCAGG CCTGCA^GG
7379 CCTGCAGG
SfiI 2515 GGCCTGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3005 GGCCATCAGGGCC
SphI 2826 GCATGC GCATG^C
4353 GCATGC
Sse8387I 6147 CCTGCAGG CCTGCA^GG
7379 CCTGCAGG
Van91I 7773 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8765 CCACCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflII AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BfrI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI XmnI