##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BclI BfrI BlnI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NspV
PacI PflFI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SwaI Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 293 GACGTC GACGT^C
AccI 2621 GTAGAC GT^[AC][GT]AC
AcsI 3756 AAATTC [GA]^AATT[CT]
AgeI 411 ACCGGT A^CCGGT
ApoI 3756 AAATTC [GA]^AATT[CT]
AviII 793 TGCGCA TGC^GCA
BbeI 1679 GGCGCC GGCGC^C
BcgI 3394 GCATAGGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1542 GGATAC GTATCC(6/5)
BsmBI 291 GAGACG CGTCTC(1/5)
BsmI 462 GAATGC GAATGC(1/-1)
Bsp1407I 3830 TGTACA T^GTACA
BspLU11I 3881 ACATGT A^CATGT
BsrFI 411 ACCGGT [GA]^CCGG[CT]
BsrGI 3830 TGTACA T^GTACA
BssHII 2370 GCGCGC G^CGCGC
BssSI 1494 CACGAG CACGAG(-5/-1)
Cfr10I 411 ACCGGT [GA]^CCGG[CT]
FspI 793 TGCGCA TGC^GCA
HindIII 2833 AAGCTT A^AGCTT
KasI 1679 GGCGCC G^GCGCC
NarI 1679 GGCGCC GG^CGCC
NdeI 2797 CATATG CA^TATG
NheI 1454 GCTAGC G^CTAGC
PaeR7I 2604 CTCGAG C^TCGAG
PinAI 411 ACCGGT A^CCGGT
SapI 198 GAAGAGC GCTCTTC(1/4)
SbfI 1280 CCTGCAGG CCTGCA^GG
ScaI 1078 AGTACT AGT^ACT
SfoI 1679 GGCGCC GGC^GCC
SphI 2629 GCATGC GCATG^C
Sse8387I 1280 CCTGCAGG CCTGCA^GG
SspBI 3830 TGTACA T^GTACA
XhoI 2604 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 123 CGCG CG^CG
2371 CGCG
AcyI 293 GACGTC G[GA]^CG[CT]C
1679 GGCGCC
AvaI 1689 CCCGAG C^[CT]CG[GA]G
2604 CTCGAG
BsaHI 293 GACGTC G[GA]^CG[CT]C
1679 GGCGCC
BsaWI 411 ACCGGT [AT]^CCGG[AT]
1867 ACCGGA
BsoBI 1689 CCCGAG C^[CT]CG[GA]G
2604 CTCGAG
BspMI 1189 ACCTGC ACCTGC(4/8)
1279 ACCTGC
BsrBI 1881 CCGCTC CCGCTC(-3/-3)
828 GAGCGG
BstAPI 1009 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3569 GCACTGGCTGC
BstEII 236 GGTCACC G^GT[ACGT]ACC
2609 GGTCACC
BstPI 236 GGTCACC G^GT[ACGT]ACC
2609 GGTCACC
BstUI 123 CGCG CG^CG
2371 CGCG
BstXI 645 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2308 CCACCCTCCTGG
EcoO65I 236 GGTCACC G^GT[ACGT]ACC
2609 GGTCACC
EcoT22I 464 ATGCAT ATGCA^T
1335 ATGCAT
Hin1I 293 GACGTC G[GA]^CG[CT]C
1679 GGCGCC
MvnI 123 CGCG CG^CG
2371 CGCG
NsiI 464 ATGCAT ATGCA^T
1335 ATGCAT
NspI 2629 GCATGC [GA]CATG^[CT]
3881 ACATGT
PflMI 298 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2033 CCACTCCCTGG
PshAI 1589 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3047 GACTCTCGTC
SspI 983 AATATT AAT^ATT
3436 AATATT
StuI 917 AGGCCT AGG^CCT
3301 AGGCCT
Van91I 298 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2033 CCACTCCCTGG
XcmI 100 CCAGTGTGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
356 CCAGTCCCAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII