##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AocI ApaI
AscI Asp718I AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BcnI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI EaeI EagI
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NarI NciI NdeI NheI
NotI NruI NsiI NspV PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 929 AACGTT AA^CGTT
AflII 1395 CTTAAG C^TTAAG
AhdI 2626 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1249 GTGCAC G^TGCAC
Aor51HI 1660 AGCGCT AGC^GCT
ApaLI 1249 GTGCAC G^TGCAC
AspEI 2626 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1967 GGTCC G^G[AT]CC
BamHI 2991 GGATCC G^GATCC
BanI 1680 GGCACC G^G[CT][GA]CC
BanII 923 GAGCCC G[GA]GC[CT]^C
BbsI 1958 GTCTTC GAAGAC(2/6)
BclI 236 TGATCA T^GATCA
BfrI 1395 CTTAAG C^TTAAG
BglI 1848 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1958 GTCTTC GAAGAC(2/6)
BsiWI 260 CGTACG C^GTACG
BsrDI 856 GCAATG GCAATG(2/0)
BsrFI 908 GCCGGC [GA]^CCGG[CT]
BssSI 704 CTCGTG CACGAG(-5/-1)
BstXI 2032 CCAACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1661 GCGC GCG^C
Cfr10I 908 GCCGGC [GA]^CCGG[CT]
DraII 115 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1617 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 786 GACTCAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2626 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1660 AGCGCT AGC^GCT
EcoNI 1923 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 115 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 236 TGATCA T^GATCA
HaeII 1660 AGCGCT [GA]GCGC^[CT]
HapII 909 CCGG C^CGG
HhaI 1661 GCGC GCG^C
HinP1I 1661 GCGC G^CGC
HpaI 1016 GTTAAC GTT^AAC
HpaII 909 CCGG C^CGG
MfeI 620 CAATTG C^AATTG
MspA1I 2833 CAGCTG C[AC]G^C[GT]G
MspI 909 CCGG C^CGG
MunI 620 CAATTG C^AATTG
NaeI 908 GCCGGC GCC^GGC
NcoI 2460 CCATGG C^CATGG
NgoMIV 908 GCCGGC G^CCGGC
PacI 681 TTAATTAA TTAAT^TAA
Psp1406I 929 AACGTT AA^CGTT
PvuII 2833 CAGCTG CAG^CTG
SapI 2838 GAAGAGC GCTCTTC(1/4)
ScaI 2700 AGTACT AGT^ACT
SphI 3257 GCATGC GCATG^C
StuI 1005 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 1142 GTGCTC G[AT]GC[AT]^C
1249 GTGCAC
BsiHKAI 1142 GTGCTC G[AT]GC[AT]^C
1249 GTGCAC
BspHI 2167 TCATGA T^CATGA
2731 TCATGA
BspMI 740 ACCTGC ACCTGC(4/8)
1086 GCAGGT
DsaI 1647 CCACGG C^C[GA][CT]GG
2460 CCATGG
EcoRI 2735 GAATTC G^AATTC
3060 GAATTC
HincII 512 GTCAAC GT[CT]^[GA]AC
1016 GTTAAC
HindII 512 GTCAAC GT[CT]^[GA]AC
1016 GTTAAC
NspI 1889 ACATGC [GA]CATG^[CT]
3257 GCATGC
PstI 958 CTGCAG CTGCA^G
1859 CTGCAG
RcaI 2167 TCATGA T^CATGA
2731 TCATGA
SpeI 780 ACTAGT A^CTAGT
1554 ACTAGT
SspI 1216 AATATT AAT^ATT
3235 AATATT
XcmI 180 CCAGCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1370 CCATGAACAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI