##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII BamHI
BbeI BcgI BfrI BglI BsaHI
BsiWI BspDI BspHI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoT22I FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MluI NarI NruI NsiI NspV
PacI PflFI PmeI PpuMI PshAI
PvuI RcaI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 471 TCCGGA T^CCGGA
AclI 2780 AACGTT AA^CGTT
ApaI 3143 GGGCCC GGGCC^C
AseI 160 ATTAAT AT^TAAT
AsnI 160 ATTAAT AT^TAAT
AvrII 731 CCTAGG C^CTAGG
BbrPI 1011 CACGTG CAC^GTG
BbvCI 1828 GCTGAGG CCTCAGC(-5/-2)
BciVI 891 GTATCC GTATCC(6/5)
BglII 1131 AGATCT A^GATCT
BlnI 731 CCTAGG C^CTAGG
BsaAI 1011 CACGTG [CT]AC^GT[GA]
BsaBI 2665 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 471 TCCGGA T^CCGGA
BsgI 1875 CTGCAC GTGCAG(16/14)
BsiEI 607 CGGCCG CG[GA][CT]^CG
BsmBI 1859 GAGACG CGTCTC(1/5)
Bsp1407I 263 TGTACA T^GTACA
BspEI 471 TCCGGA T^CCGGA
BspLU11I 2924 ACATGT A^CATGT
BsrGI 263 TGTACA T^GTACA
BstAPI 1699 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 473 CGGACCG CG^G[AT]CCG
DraI 3431 TTTAAA TTT^AAA
DraII 3142 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 2525 GACTTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 607 CGGCCG C^GGCCG
EclXI 607 CGGCCG C^GGCCG
Eco52I 607 CGGCCG C^GGCCG
EcoO109I 3142 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 373 GAATTC G^AATTC
EcoRV 2264 GATATC GAT^ATC
HgaI 314 GACGC GACGC(5/10)
HincII 2362 GTCAAC GT[CT]^[GA]AC
HindII 2362 GTCAAC GT[CT]^[GA]AC
MamI 2665 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1810 CAATTG C^AATTG
MroI 471 TCCGGA T^CCGGA
MunI 1810 CAATTG C^AATTG
NaeI 836 GCCGGC GCC^GGC
NcoI 2856 CCATGG C^CATGG
NdeI 3232 CATATG CA^TATG
NgoMIV 836 GCCGGC G^CCGGC
NotI 606 GCGGCCGC GC^GGCCGC
NspI 2924 ACATGT [GA]CATG^[CT]
PflMI 2644 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1011 CACGTG CAC^GTG
PmlI 1011 CACGTG CAC^GTG
PshBI 160 ATTAAT AT^TAAT
Psp1406I 2780 AACGTT AA^CGTT
RsrII 473 CGGACCG CG^G[AT]CCG
SapI 3237 GAAGAGC GCTCTTC(1/4)
SmaI 631 CCCGGG CCC^GGG
SspBI 263 TGTACA T^GTACA
Van91I 2644 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1552 TCTAGA T^CTAGA
XmaCI 631 CCCGGG C^CCGGG
XmaI 631 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1862 ACGTGT A^C[GA][CT]GT
2924 ACATGT
AgeI 18 ACCGGT A^CCGGT
646 ACCGGT
AlwNI 1736 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3218 CAGAGGCTG
BalI 1743 TGGCCA TGG^CCA
2538 TGGCCA
BanI 876 GGCACC G^G[CT][GA]CC
2657 GGCACC
BclI 414 TGATCA T^GATCA
3196 TGATCA
BlpI 745 GCTGAGC GC^T[ACGT]AGC
3035 GCTGAGC
Bpu1102I 745 GCTGAGC GC^T[ACGT]AGC
3035 GCTGAGC
BsaI 2013 GAGACC GGTCTC(1/5)
3382 GGTCTC
BseRI 1526 GAGGAG GAGGAG(10/8)
2010 GAGGAG
BsmI 2293 GAATGC GAATGC(1/-1)
2872 GAATGC
BsrBI 625 CCGCTC CCGCTC(-3/-3)
1937 CCGCTC
BsrDI 1805 CATTGC GCAATG(2/0)
3533 CATTGC
CelII 745 GCTGAGC GC^T[ACGT]AGC
3035 GCTGAGC
DsaI 2184 CCGTGG C^C[GA][CT]GG
2856 CCATGG
FauI 624 CCCGC CCCGC(4/6)
739 GCGGG
FbaI 414 TGATCA T^GATCA
3196 TGATCA
MluNI 1743 TGGCCA TGG^CCA
2538 TGGCCA
MscI 1743 TGGCCA TGG^CCA
2538 TGGCCA
NheI 2297 GCTAGC G^CTAGC
3113 GCTAGC
PaeR7I 1842 CTCGAG C^TCGAG
2007 CTCGAG
PinAI 18 ACCGGT A^CCGGT
646 ACCGGT
SacI 883 GAGCTC GAGCT^C
1125 GAGCTC
XhoI 1842 CTCGAG C^TCGAG
2007 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EarI Eco81I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI