##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I BcgI BfrI BlpI
Bpu1102I BsiEI BsiWI BspHI BssHII
BstBI CelII CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FseI HpaI KpnI
MfeI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 2092 GACGTC GACGT^C
AccIII 1493 TCCGGA T^CCGGA
AhdI 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2530 TGCGCA TGC^GCA
AvrII 2731 CCTAGG C^CTAGG
BamHI 1239 GGATCC G^GATCC
BbeI 2293 GGCGCC GGCGC^C
BbrPI 988 CACGTG CAC^GTG
BbvCI 2965 GCTGAGG CCTCAGC(-5/-2)
BciVI 2878 GTATCC GTATCC(6/5)
BclI 1295 TGATCA T^GATCA
BglI 1166 GCCCCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2731 CCTAGG C^CTAGG
BsaAI 988 CACGTG [CT]AC^GT[GA]
BsaBI 2127 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1493 TCCGGA T^CCGGA
BsmI 2573 GAATGC GAATGC(1/-1)
Bsp1407I 3168 TGTACA T^GTACA
BspDI 689 ATCGAT AT^CGAT
BspEI 1493 TCCGGA T^CCGGA
BsrDI 3018 CATTGC GCAATG(2/0)
BsrGI 3168 TGTACA T^GTACA
BssSI 2046 CACGAG CACGAG(-5/-1)
Bst1107I 952 GTATAC GTA^TAC
BstAPI 2553 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2078 GGTCACC G^GT[ACGT]ACC
BstPI 2078 GGTCACC G^GT[ACGT]ACC
BstZ17I 952 GTATAC GTA^TAC
ClaI 689 ATCGAT AT^CGAT
Eam1105I 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1722 GAAGAG CTCTTC(1/4)
EcoO65I 2078 GGTCACC G^GT[ACGT]ACC
EcoRI 853 GAATTC G^AATTC
FbaI 1295 TGATCA T^GATCA
FspI 2530 TGCGCA TGC^GCA
HincII 1554 GTCGAC GT[CT]^[GA]AC
HindII 1554 GTCGAC GT[CT]^[GA]AC
HindIII 790 AAGCTT A^AGCTT
KasI 2293 GGCGCC G^GCGCC
Ksp632I 1722 GAAGAG CTCTTC(1/4)
KspI 29 CCGCGG CCGC^GG
MamI 2127 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1354 ACGCGT A^CGCGT
MroI 1493 TCCGGA T^CCGGA
MseI 2930 TTAA T^TAA
NarI 2293 GGCGCC GG^CGCC
NheI 2177 GCTAGC G^CTAGC
PmaCI 988 CACGTG CAC^GTG
PmlI 988 CACGTG CAC^GTG
PpuMI 2108 GGGACCC [GA]G^G[AT]CC[CT]
SacII 29 CCGCGG CCGC^GG
SalI 1554 GTCGAC G^TCGAC
SexAI 521 ACCTGGT A^CC[AT]GGT
SfiI 1165 GGCCCCGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2293 GGCGCC GGC^GCC
SmaI 2769 CCCGGG CCC^GGG
SphI 1131 GCATGC GCATG^C
SspBI 3168 TGTACA T^GTACA
Tru9I 2930 TTAA T^TAA
XmaCI 2769 CCCGGG C^CCGGG
XmaI 2769 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 824 GTAC GT^AC
3169 GTAC
AlwNI 2285 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2429 CAGGGACTG
ApaI 310 GGGCCC GGGCC^C
2315 GGGCCC
AspI 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1060 GACATCGTC
BbsI 1749 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BglII 177 AGATCT A^GATCT
1888 AGATCT
BpuAI 1749 GAAGAC GAAGAC(2/6)
1075 GTCTTC
BsaI 1188 GGTCTC GGTCTC(1/5)
2973 GGTCTC
BsgI 1147 GTGCAG GTGCAG(16/14)
2560 GTGCAG
BspLU11I 747 ACATGT A^CATGT
3148 ACATGT
BsrBI 1348 GAGCGG CCGCTC(-3/-3)
2049 GAGCGG
BsrFI 1609 GCCGGC [GA]^CCGG[CT]
2378 GCCGGC
Cfr10I 1609 GCCGGC [GA]^CCGG[CT]
2378 GCCGGC
NaeI 1609 GCCGGC GCC^GGC
2378 GCCGGC
NcoI 1511 CCATGG C^CATGG
1520 CCATGG
NgoMIV 1609 GCCGGC G^CCGGC
2378 GCCGGC
PflFI 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1060 GACATCGTC
RsaI 824 GTAC GT^AC
3169 GTAC
TfiI 452 GAATC G^A[AT]TC
692 GATTC
Tth111I 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1060 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AflIII AluI AlwI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII