################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I BcgI BfrI BlpI Bpu1102I BsiEI BsiWI BspHI BssHII BstBI CelII CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI HpaI KpnI MfeI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SapI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 2092 GACGTC GACGT^C AccIII 1493 TCCGGA T^CCGGA AhdI 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2530 TGCGCA TGC^GCA AvrII 2731 CCTAGG C^CTAGG BamHI 1239 GGATCC G^GATCC BbeI 2293 GGCGCC GGCGC^C BbrPI 988 CACGTG CAC^GTG BbvCI 2965 GCTGAGG CCTCAGC(-5/-2) BciVI 2878 GTATCC GTATCC(6/5) BclI 1295 TGATCA T^GATCA BglI 1166 GCCCCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2731 CCTAGG C^CTAGG BsaAI 988 CACGTG [CT]AC^GT[GA] BsaBI 2127 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1493 TCCGGA T^CCGGA BsmI 2573 GAATGC GAATGC(1/-1) Bsp1407I 3168 TGTACA T^GTACA BspDI 689 ATCGAT AT^CGAT BspEI 1493 TCCGGA T^CCGGA BsrDI 3018 CATTGC GCAATG(2/0) BsrGI 3168 TGTACA T^GTACA BssSI 2046 CACGAG CACGAG(-5/-1) Bst1107I 952 GTATAC GTA^TAC BstAPI 2553 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2078 GGTCACC G^GT[ACGT]ACC BstPI 2078 GGTCACC G^GT[ACGT]ACC BstZ17I 952 GTATAC GTA^TAC ClaI 689 ATCGAT AT^CGAT Eam1105I 585 GACCAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1722 GAAGAG CTCTTC(1/4) EcoO65I 2078 GGTCACC G^GT[ACGT]ACC EcoRI 853 GAATTC G^AATTC FbaI 1295 TGATCA T^GATCA FspI 2530 TGCGCA TGC^GCA HincII 1554 GTCGAC GT[CT]^[GA]AC HindII 1554 GTCGAC GT[CT]^[GA]AC HindIII 790 AAGCTT A^AGCTT KasI 2293 GGCGCC G^GCGCC Ksp632I 1722 GAAGAG CTCTTC(1/4) KspI 29 CCGCGG CCGC^GG MamI 2127 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1354 ACGCGT A^CGCGT MroI 1493 TCCGGA T^CCGGA MseI 2930 TTAA T^TAA NarI 2293 GGCGCC GG^CGCC NheI 2177 GCTAGC G^CTAGC PmaCI 988 CACGTG CAC^GTG PmlI 988 CACGTG CAC^GTG PpuMI 2108 GGGACCC [GA]G^G[AT]CC[CT] SacII 29 CCGCGG CCGC^GG SalI 1554 GTCGAC G^TCGAC SexAI 521 ACCTGGT A^CC[AT]GGT SfiI 1165 GGCCCCGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2293 GGCGCC GGC^GCC SmaI 2769 CCCGGG CCC^GGG SphI 1131 GCATGC GCATG^C SspBI 3168 TGTACA T^GTACA Tru9I 2930 TTAA T^TAA XmaCI 2769 CCCGGG C^CCGGG XmaI 2769 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 824 GTAC GT^AC 3169 GTAC AlwNI 2285 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 2429 CAGGGACTG ApaI 310 GGGCCC GGGCC^C 2315 GGGCCC AspI 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1060 GACATCGTC BbsI 1749 GAAGAC GAAGAC(2/6) 1075 GTCTTC BglII 177 AGATCT A^GATCT 1888 AGATCT BpuAI 1749 GAAGAC GAAGAC(2/6) 1075 GTCTTC BsaI 1188 GGTCTC GGTCTC(1/5) 2973 GGTCTC BsgI 1147 GTGCAG GTGCAG(16/14) 2560 GTGCAG BspLU11I 747 ACATGT A^CATGT 3148 ACATGT BsrBI 1348 GAGCGG CCGCTC(-3/-3) 2049 GAGCGG BsrFI 1609 GCCGGC [GA]^CCGG[CT] 2378 GCCGGC Cfr10I 1609 GCCGGC [GA]^CCGG[CT] 2378 GCCGGC NaeI 1609 GCCGGC GCC^GGC 2378 GCCGGC NcoI 1511 CCATGG C^CATGG 1520 CCATGG NgoMIV 1609 GCCGGC G^CCGGC 2378 GCCGGC PflFI 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1060 GACATCGTC RsaI 824 GTAC GT^AC 3169 GTAC TfiI 452 GAATC G^A[AT]TC 692 GATTC Tth111I 942 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1060 GACATCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AflIII AluI AlwI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII