##################################
# NO CUT ENZYMES ( 200 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NlaIII NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AciI 258 CCGC CCGC(-3/-1)
AluI 75 AGCT AG^CT
AlwNI 267 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 167 CCTCAGG CC^T[ACGT]AGG
BbsI 109 GAAGAC GAAGAC(2/6)
BbvI 22 GCTGC GCAGC(8/12)
BcnI 236 CCGGG CC^[GC]GG
BpmI 64 CTCCAG CTGGAG(16/14)
BpuAI 109 GAAGAC GAAGAC(2/6)
BsmFI 228 GTCCC GGGAC(10/14)
BspMI 190 ACCTGC ACCTGC(4/8)
BsrI 205 ACTGG ACTGG(1/-1)
BstF5I 15 CATCC GGATG(2/0)
BstXI 26 CCATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 167 CCTCAGG CC^T[ACGT]AGG
Cac8I 45 GCAGGC GC[ACGT]^[ACGT]GC
DraII 119 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 269 GACTCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 167 CCTCAGG CC^T[ACGT]AGG
EcoO109I 119 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 15 CATCC GGATG(9/13)
HapII 236 CCGG C^CGG
HpaII 236 CCGG C^CGG
MaeIII 60 GTCAC ^GT[ACGT]AC
MboII 109 GAAGA GAAGA(8/7)
MspI 236 CCGG C^CGG
NciI 236 CCGGG CC^[GC]GG
PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT]
PstI 43 CTGCAG CTGCA^G
SbfI 42 CCTGCAGG CCTGCA^GG
SfiI 39 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 42 CCTGCAGG CCTGCA^GG
TseI 22 GCTGC G^C[AT]GC
Tsp45I 60 GTCAC ^GT[GC]AC
TspRI 252 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AvaII 5 GGACC G^G[AT]CC
120 GGACC
BglI 40 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
200 GCCGAACTGGC
BsaJI 50 CCCAGG C^C[ACGT][ACGT]GG
236 CCGGGG
BsiYI 230 CCCTCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCTCTCCGGGG
BslI 230 CCCTCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCTCTCCGGGG
EaeI 207 TGGCCG [CT]^GGCC[GA]
255 TGGCCG
Fnu4HI 22 GCTGC GC^[ACGT]GC
257 GCCGC
ItaI 22 GCTGC GC^[ACGT]GC
257 GCCGC
SfcI 43 CTGCAG C^T[GA][CT]AG
264 CTACAG
XcmI 26 CCATTCTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
93 CCAGAAGCAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BssKI BstNI Cfr13I DdeI EcoRII
HaeIII HinfI MnlI MvaI MwoI
PleI Sau96I ScrFI