##################################
# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AciI 112 GCGG CCGC(-3/-1)
AvaII 5 GGACC G^G[AT]CC
BbsI 260 GAAGAC GAAGAC(2/6)
BcnI 96 CCCGG CC^[GC]GG
BglI 40 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 55 GCTGAGC GC^T[ACGT]AGC
BpmI 64 CTCCAG CTGGAG(16/14)
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
BpuAI 260 GAAGAC GAAGAC(2/6)
BsmFI 146 GGGAC GGGAC(10/14)
BsmI 271 GCATTC GAATGC(1/-1)
BspMI 244 ACCTGC ACCTGC(4/8)
BsrDI 219 CATTGC GCAATG(2/0)
BstXI 26 CCATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 55 GCTGAGC GC^T[ACGT]AGC
DdeI 56 CTGAG C^T[ACGT]AG
DraII 4 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 4 AGGACCC [GA]G^G[ACGT]CC[CT]
HapII 97 CCGG C^CGG
HindIII 153 AAGCTT A^AGCTT
HinfI 263 GACTC G^A[ACGT]TC
HpaII 97 CCGG C^CGG
MaeII 231 ACGT A^CGT
MboII 260 GAAGA GAAGA(8/7)
MspA1I 110 CAGCGG C[AC]G^C[GT]G
MspI 97 CCGG C^CGG
NciI 96 CCCGG CC^[GC]GG
PleI 263 GACTC GAGTC(4/5)
PpuMI 4 AGGACCC [GA]G^G[AT]CC[CT]
SbfI 42 CCTGCAGG CCTGCA^GG
SfiI 39 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 177 CTCAAG C^T[CT][GA]AG
Sse8387I 42 CCTGCAGG CCTGCA^GG
XcmI 26 CCATTCTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 5 enzymes) #
###################################
AluI 75 AGCT AG^CT
154 AGCT
NlaIII 143 CATG CATG^
189 CATG
PstI 43 CTGCAG CTGCA^G
89 CTGCAG
SfaNI 168 GCATC GCATC(5/9)
248 GCATC
SfcI 43 CTGCAG C^T[GA][CT]AG
89 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I EcoRII
Fnu4HI FokI HaeIII ItaI MnlI
MvaI MwoI NlaIV Sau96I ScrFI
TseI