##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbrPI BbsI BciVI
BclI BfaI BfrI BlnI BpuAI
BsaAI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 385 GTATAC GT^[AC][GT]AC
AccIII 335 TCCGGA T^CCGGA
AcyI 2055 GGCGCC G[GA]^CG[CT]C
AflIII 227 ACGTGT A^C[GA][CT]GT
Alw44I 2252 GTGCAC G^TGCAC
AocI 2230 CCTCAGG CC^T[ACGT]AGG
ApaLI 2252 GTGCAC G^TGCAC
AspI 930 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 746 TGGCCA TGG^CCA
BamHI 2235 GGATCC G^GATCC
BbeI 2055 GGCGCC GGCGC^C
BcgI 536 CGAATGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1979 AGATCT A^GATCT
BsaHI 2055 GGCGCC G[GA]^CG[CT]C
BsaWI 335 TCCGGA [AT]^CCGG[AT]
BseAI 335 TCCGGA T^CCGGA
BsmI 339 GAATGC GAATGC(1/-1)
BspEI 335 TCCGGA T^CCGGA
BspHI 1268 TCATGA T^CATGA
BsrFI 2256 ACCGGC [GA]^CCGG[CT]
Bst1107I 385 GTATAC GTA^TAC
BstZ17I 385 GTATAC GTA^TAC
Bsu36I 2230 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2256 ACCGGC [GA]^CCGG[CT]
DraIII 1774 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 192 CGGCCG C^GGCCG
EclXI 192 CGGCCG C^GGCCG
Eco52I 192 CGGCCG C^GGCCG
Eco81I 2230 CCTCAGG CC^T[ACGT]AGG
HgaI 941 GCGTC GACGC(5/10)
Hin1I 2055 GGCGCC G[GA]^CG[CT]C
HincII 1653 GTTGAC GT[CT]^[GA]AC
HindII 1653 GTTGAC GT[CT]^[GA]AC
KasI 2055 GGCGCC G^GCGCC
MluNI 746 TGGCCA TGG^CCA
MroI 335 TCCGGA T^CCGGA
MscI 746 TGGCCA TGG^CCA
NarI 2055 GGCGCC GG^CGCC
PaeR7I 93 CTCGAG C^TCGAG
PflFI 930 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 71 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2190 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1268 TCATGA T^CATGA
SfiI 705 GGCCTTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2055 GGCGCC GGC^GCC
SmlI 93 CTCGAG C^T[CT][GA]AG
SphI 1769 GCATGC GCATG^C
StuI 1712 AGGCCT AGG^CCT
Tth111I 930 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 93 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 1792 GGGCCC GGGCC^C
1853 GGGCCC
BbvCI 1829 CCTCAGC CCTCAGC(-5/-2)
2113 CCTCAGC
BlpI 180 GCTGAGC GC^T[ACGT]AGC
673 GCTGAGC
BpmI 1931 CTCCAG CTGGAG(16/14)
1632 CTGGAG
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
673 GCTGAGC
BsaI 1339 GAGACC GGTCTC(1/5)
2196 GGTCTC
BsiEI 107 CGACCG CG[GA][CT]^CG
192 CGGCCG
BssSI 1729 CACGAG CACGAG(-5/-1)
316 CTCGTG
BstAPI 641 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1120 GCAATCCTTGC
BstYI 1979 AGATCT [GA]^GATC[CT]
2235 GGATCC
CelII 180 GCTGAGC GC^T[ACGT]AGC
673 GCTGAGC
DsaI 1796 CCGTGG C^C[GA][CT]GG
2264 CCGTGG
EcoNI 435 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1643 CCTGGTGCAGG
HaeII 2000 AGCGCC [GA]GCGC^[CT]
2055 GGCGCC
MflI 1979 AGATCT [GA]^GATC[CT]
2235 GGATCC
SacI 31 GAGCTC GAGCT^C
1732 GAGCTC
SexAI 1090 ACCTGGT A^CC[AT]GGT
1642 ACCTGGT
SmaI 75 CCCGGG CCC^GGG
1300 CCCGGG
TfiI 777 GAATC G^A[AT]TC
1530 GAATC
XhoII 1979 AGATCT [GA]^GATC[CT]
2235 GGATCC
XmaCI 75 CCCGGG C^CCGGG
1300 CCCGGG
XmaI 75 CCCGGG C^CCGGG
1300 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI