Miyakogusa Predicted Gene
- Lj6g3v1333200.1
BLASTN 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lj6g3v1333200.1 tr|G7INL3|G7INL3_MEDTR Subtilisin-like serine
protease OS=Medicago truncatula GN=MTR_2g037850 PE=3
S,86.92,0,SUBTILISIN,Peptidase S8, subtilisin-related;
SUBTILASE_ASP,Peptidase S8, subtilisin, Asp-active sit,CUFF.59375.1
(2109 letters)
Database: LJGI
47,486 sequences; 32,788,469 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gnl|LJGI|DC597264 similar to UniRef100_Q4U4M3 Cluster: Subtilisi... 1068 0.0
gnl|LJGI|BP042339 similar to UniRef100_A7QXN3 Cluster: Chromosom... 464 e-129
gnl|LJGI|TC69971 similar to UniRef100_A7QXN3 Cluster: Chromosome... 446 e-124
gnl|LJGI|TC61463 similar to UniRef100_A7QXN3 Cluster: Chromosome... 446 e-124
gnl|LJGI|TC75669 similar to UniRef100_Q4U4M3 Cluster: Subtilisin... 216 4e-55
gnl|LJGI|TC81918 similar to UniRef100_A7QXN3 Cluster: Chromosome... 135 1e-30
gnl|LJGI|BP066611 similar to UniRef100_A7QXN3 Cluster: Chromosom... 117 3e-25
gnl|LJGI|TC67986 similar to UniRef100_A7P111 Cluster: Chromosome... 103 4e-21
gnl|LJGI|TC81905 similar to UniRef100_A7QXN3 Cluster: Chromosome... 98 3e-19
gnl|LJGI|TC75277 similar to UniRef100_Q4U4M3 Cluster: Subtilisin... 98 3e-19
gnl|LJGI|TC65862 similar to UniRef100_Q4U4M3 Cluster: Subtilisin... 98 3e-19
>gnl|LJGI|DC597264 similar to UniRef100_Q4U4M3 Cluster: Subtilisin-like protease; n=1;
Arachis hypogaea|Rep: Subtilisin-like protease - Arachis
hypogaea (Peanut), partial (35%)
Length = 566
Score = 1068 bits (539), Expect = 0.0
Identities = 557/566 (98%)
Strand = Plus / Plus
Query: 889 caggctgtacatgatggagtggatatactcagcctttcagttggaccaaacagtccttca 948
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1 caggctgtacatgatggagtggatatactcagcctttcagttggaccaaacagtccttca 60
Query: 949 acaacaaccaaaacaacgtttttgaatccttttgatgctacacttcttggagctgtgaaa 1008
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61 acaacaaccaaaacaacgtttttgaatccttttgatgctacacttcttggagctgtgaaa 120
Query: 1009 gctggtgtatttgttgcacaggctgctggaaatggtggtccttttcctaagacattagtt 1068
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gctggtgtatttgttgcacaggctgctggaaatggtggtccttttcctaagacattagtt 180
Query: 1069 tcatacagtccatggatagcatctgtaggagctgcaattgatgatcgtagatacaaaaac 1128
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 tcatacagtccatggatagcatctgtaggagctgcaattgatgatcgtagatacaaaaac 240
Query: 1129 catctaacccttggaaatggacacatcttagcaggacttgggctgtcaccttctacacgt 1188
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 catctaacccttggaaatggacacatcttagcaggacttgggctgtcaccttctacacgt 300
Query: 1189 ttaaacaaaacatacacgttggttgctgcaaatgatgttctgctagattcctcagttatg 1248
||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 301 ttaaacaaaacatacacgttggttgctgcnnatgatgttctgctagattcctcagttatg 360
Query: 1249 aagttcagtcccacggattgccagagaccagaagttttaaacaagaacttgataaagggg 1308
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 aagttcagtcccacggattgccagagaccagaagttttaaacaagaacttgataaagggg 420
Query: 1309 aatattcttctctgtggctattcatacaactttgtttctggtactgcatcaatnnnnnnn 1368
|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 aatattcttctctgtggctattcatacaactttgtttctggtactgcatcaataaaaaaa 480
Query: 1369 gtctctgaaacagcaaaggcccttggagcagttggatttgttctttgtgtagaaaatgtt 1428
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 gtctctgaaacagcaaaggcccttggagcagttggatttgttctttgtgtagaaaatgtt 540
Query: 1429 tctcctggaatgaaatttgatccagt 1454
||||||||||||||||||||||||||
Sbjct: 541 tctcctggaatgaaatttgatccagt 566
>gnl|LJGI|BP042339 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226, whole
genome shotgun sequence - Vitis vinifera (Grape), partial
(18%)
Length = 447
Score = 464 bits (234), Expect = e-129
Identities = 380/431 (88%)
Strand = Plus / Plus
Query: 1000 gctgtgaaagctggtgtatttgttgcacaggctgctggaaatggtggtccttttcctaag 1059
|||||||||||||||||||||||||||||||||||||||||||| ||||| |||||||||
Sbjct: 1 gctgtgaaagctggtgtatttgttgcacaggctgctggaaatggcggtccatttcctaag 60
Query: 1060 acattagtttcatacagtccatggatagcatctgtaggagctgcaattgatgatcgtaga 1119
||| | |||||||| |||||||||||| ||||||||| ||||||||||||||||||||||
Sbjct: 61 acaatggtttcatatagtccatggataacatctgtagcagctgcaattgatgatcgtaga 120
Query: 1120 tacaaaaaccatctaacccttggaaatggacacatcttagcaggacttgggctgtcacct 1179
|| ||||||||| | |||||||| |||||| | |||||||| ||| | ||| | ||||||
Sbjct: 121 tataaaaaccatttgacccttgggaatggaaaaatcttagctggaatcgggttatcacct 180
Query: 1180 tctacacgtttaaacaaaacatacacgttggttgctgcaaatgatgttctgctagattcc 1239
||||||| ||||||| ||||||||| ||||||||||||||||||||| |||||||||||
Sbjct: 181 tctacacatttaaaccaaacatacaagttggttgctgcaaatgatgtgctgctagattct 240
Query: 1240 tcagttatgaagttcagtcccacggattgccagagaccagaagttttaaacaagaacttg 1299
||| |||| |||| ||| ||||| |||||||||||||||||||||||||||||||| |||
Sbjct: 241 tcacttattaagtacagccccacagattgccagagaccagaagttttaaacaagaaattg 300
Query: 1300 ataaaggggaatattcttctctgtggctattcatacaactttgtttctggtactgcatca 1359
||| |||| ||||||||||||||||| ||||| | |||||||||| |||||||||||||
Sbjct: 301 atagagggaaatattcttctctgtggatattctttcaactttgttgttggtactgcatca 360
Query: 1360 atnnnnnnngtctctgaaacagcaaaggcccttggagcagttggatttgttctttgtgta 1419
|| ||||| |||||||||||| ||||||| || | |||||||||||| |||||
Sbjct: 361 atcaagaaagtctcagaaacagcaaagacccttggggctgctggatttgttctctgtgtt 420
Query: 1420 gaaaatgtttc 1430
||||| |||||
Sbjct: 421 gaaaacgtttc 431
>gnl|LJGI|TC69971 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226,
whole genome shotgun sequence - Vitis vinifera (Grape),
partial (21%)
Length = 635
Score = 446 bits (225), Expect = e-124
Identities = 396/453 (87%)
Strand = Plus / Plus
Query: 73 gaggtgtacatagtaacagttgaaggtgagcctatcataagttatatgggcggcatcgat 132
||||| ||||||||||| |||||||||||||||||||| ||||| || || || |||
Sbjct: 174 gaggtttacatagtaactgttgaaggtgagcctatcatcagttaccgtggaggaattgat 233
Query: 133 ggttttgaagctactgctgtggaatctgatgagaagattgatacctcaagggaatccgtc 192
|| |||||||||||||||||||||||||||||||||||||| ||| |||| |||| ||
Sbjct: 234 gggtttgaagctactgctgtggaatctgatgagaagattgacaccacaagtgaattggtt 293
Query: 193 acttcttatgcccgccaccttgaaaatagacatgatatgcttctggggatgctttttgat 252
||||||||||| || || |||||||| |||||||| ||| ||||||||||||| |||||
Sbjct: 294 acttcttatgctcgtcatcttgaaaagagacatgacatgattctggggatgctgtttgag 353
Query: 253 gaagggacatacaagaaactctatagctatagacatcttataaatgggtttgctgctcat 312
||| |||||||||||| ||||| ||||||| ||||||||||||||||||||||| ||||
Sbjct: 354 caagagacatacaagaagctctacagctataaacatcttataaatgggtttgctgttcat 413
Query: 313 atttccccagaacaggcagaaactcttagacatgctcctggtgtaaaatctgtaacgagg 372
|||||||||||||||||||||||||| |||| ||||||||| || |||||||| ||||
Sbjct: 414 atttccccagaacaggcagaaactctaagacgtgctcctggagtgaaatctgttgagagg 473
Query: 373 gactggaaagttaggagactcacaacacataccccacagtttttagggcttccaactggt 432
||||||||||| || | ||| || ||||||||||| || |||||||| |||||||| ||
Sbjct: 474 gactggaaagtgagaaaactgacgacacataccccgcaatttttaggccttccaaccggg 533
Query: 433 gtatggccaacaggaggtggctatgaacgggctggagaagacattgtgattggatttgtg 492
|||||||| |||||||| |||| ||| ||||||||||||||||||||||| |||||||||
Sbjct: 534 gtatggccgacaggaggcggctttgatcgggctggagaagacattgtgataggatttgtg 593
Query: 493 gactctgggatctttcctcaccacccaagcttt 525
||||| ||||||| ||| |||||||| ||||||
Sbjct: 594 gactcagggatctatccccaccaccccagcttt 626
>gnl|LJGI|TC61463 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226,
whole genome shotgun sequence - Vitis vinifera (Grape),
partial (21%)
Length = 795
Score = 446 bits (225), Expect = e-124
Identities = 396/453 (87%)
Strand = Plus / Plus
Query: 73 gaggtgtacatagtaacagttgaaggtgagcctatcataagttatatgggcggcatcgat 132
||||| ||||||||||| |||||||||||||||||||| ||||| || || || |||
Sbjct: 343 gaggtttacatagtaactgttgaaggtgagcctatcatcagttaccgtggaggaattgat 402
Query: 133 ggttttgaagctactgctgtggaatctgatgagaagattgatacctcaagggaatccgtc 192
|| |||||||||||||||||||||||||||||||||||||| ||| |||| |||| ||
Sbjct: 403 gggtttgaagctactgctgtggaatctgatgagaagattgacaccacaagtgaattggtt 462
Query: 193 acttcttatgcccgccaccttgaaaatagacatgatatgcttctggggatgctttttgat 252
||||||||||| || || |||||||| |||||||| ||| ||||||||||||| |||||
Sbjct: 463 acttcttatgctcgtcatcttgaaaagagacatgacatgattctggggatgctgtttgag 522
Query: 253 gaagggacatacaagaaactctatagctatagacatcttataaatgggtttgctgctcat 312
||| |||||||||||| ||||| ||||||| ||||||||||||||||||||||| ||||
Sbjct: 523 caagagacatacaagaagctctacagctataaacatcttataaatgggtttgctgttcat 582
Query: 313 atttccccagaacaggcagaaactcttagacatgctcctggtgtaaaatctgtaacgagg 372
|||||||||||||||||||||||||| |||| ||||||||| || |||||||| ||||
Sbjct: 583 atttccccagaacaggcagaaactctaagacgtgctcctggagtgaaatctgttgagagg 642
Query: 373 gactggaaagttaggagactcacaacacataccccacagtttttagggcttccaactggt 432
||||||||||| || | ||| || ||||||||||| || |||||||| |||||||| ||
Sbjct: 643 gactggaaagtgagaaaactgacgacacataccccgcaatttttaggccttccaaccggg 702
Query: 433 gtatggccaacaggaggtggctatgaacgggctggagaagacattgtgattggatttgtg 492
|||||||| |||||||| |||| ||| ||||||||||||||||||||||| |||||||||
Sbjct: 703 gtatggccgacaggaggcggctttgatcgggctggagaagacattgtgataggatttgtg 762
Query: 493 gactctgggatctttcctcaccacccaagcttt 525
||||| ||||||| ||| |||||||| ||||||
Sbjct: 763 gactcagggatctatccccaccaccccagcttt 795
>gnl|LJGI|TC75669 similar to UniRef100_Q4U4M3 Cluster: Subtilisin-like protease; n=1;
Arachis hypogaea|Rep: Subtilisin-like protease - Arachis
hypogaea (Peanut), partial (34%)
Length = 1138
Score = 216 bits (109), Expect = 4e-55
Identities = 178/201 (88%)
Strand = Plus / Plus
Query: 1905 aactatagacagagcaggaaatcctattctagcacagcaatattctgaaacagaagctgt 1964
|||| ||||||||||||| |||||| ||||||| |||||| |||||||||||||||| |
Sbjct: 2 aactctagacagagcagggaatcctcttctagcccagcaaacttctgaaacagaagctat 61
Query: 1965 gaagctggtcagagccactccttttgattatgggagcggacacgtgagtccaagagctgc 2024
||||||||||| |||||||||||||||||||||||| || || || ||||||||||||
Sbjct: 62 gaagctggtcaaagccactccttttgattatgggagtgggcatgttgatccaagagctgc 121
Query: 2025 cttgaaccctggtctcatctttgatgcaggatacgaggattatttaggatttttgtgcac 2084
|||| | |||||||||||||||||||||||||||||||||||| | || || || |||||
Sbjct: 122 cttggatcctggtctcatctttgatgcaggatacgaggattatctcggcttcttatgcac 181
Query: 2085 aacacctggcattgatgttca 2105
|||||| |||||||| |||||
Sbjct: 182 aacaccaggcattgacgttca 202
>gnl|LJGI|TC81918 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226, whole
genome shotgun sequence - Vitis vinifera (Grape), partial
(3%)
Length = 1084
Score = 135 bits (68), Expect = 1e-30
Identities = 160/193 (82%)
Strand = Plus / Plus
Query: 1293 gaacttgataaaggggaatattcttctctgtggctattcatacaactttgtttctggtac 1352
|||||||||| |||||| | ||||||| ||||||||||| ||||||||||||||||||||
Sbjct: 539 gaacttgatagaggggattgttcttctttgtggctattcttacaactttgtttctggtac 598
Query: 1353 tgcatcaatnnnnnnngtctctgaaacagcaaaggcccttggagcagttggatttgttct 1412
||||| ||| |||||| |||||||||| |||||| ||||||| ||| ||||||
Sbjct: 599 tgcattaataaagaaagtctctaaaacagcaaaaacccttgaagcagtttgatatgttct 658
Query: 1413 ttgtgtagaaaatgtttctcctggaatgaaatttgatccagtccctgttggccttcctgg 1472
||| |||| |||||||||||| || |||| | |||||||| | ||||||||||| ||
Sbjct: 659 ttgaatagagaatgtttctcctaaaaggaaactagatccagttcatgttggccttcttga 718
Query: 1473 gattgttatcaca 1485
||| ||||||||
Sbjct: 719 cattcttatcaca 731
Score = 89.7 bits (45), Expect = 7e-17
Identities = 67/73 (91%), Gaps = 1/73 (1%)
Strand = Plus / Plus
Query: 1181 ctacacgtttaaacaaa-acatacacgttggttgctgcaaatgatgttctgctagattcc 1239
|||||| |||||||||| |||||||| |||||||||||||||||||||||||||||||||
Sbjct: 451 ctacacatttaaacaaatacatacactttggttgctgcaaatgatgttctgctagattcc 510
Query: 1240 tcagttatgaagt 1252
||| |||||||
Sbjct: 511 caagtcatgaagt 523
>gnl|LJGI|BP066611 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226,
whole genome shotgun sequence - Vitis vinifera (Grape),
partial (8%)
Length = 478
Score = 117 bits (59), Expect = 3e-25
Identities = 113/131 (86%)
Strand = Plus / Plus
Query: 73 gaggtgtacatagtaacagttgaaggtgagcctatcataagttatatgggcggcatcgat 132
||||| ||||||||||| |||||||||||||||||||| ||||| || || || |||
Sbjct: 341 gaggtttacatagtaactgttgaaggtgagcctatcatcagttaccgtggaggaattgat 400
Query: 133 ggttttgaagctactgctgtggaatctgatgagaagattgatacctcaagggaatccgtc 192
|| ||||||||||||||||||||||||||||||||||| || ||| |||| |||| ||
Sbjct: 401 gggtttgaagctactgctgtggaatctgatgagaagatcgacaccacaagtgaattggtt 460
Query: 193 acttcttatgc 203
|||||||||||
Sbjct: 461 acttcttatgc 471
>gnl|LJGI|TC67986 similar to UniRef100_A7P111 Cluster: Chromosome chr19 scaffold_4,
whole genome shotgun sequence; n=1; Vitis vinifera|Rep:
Chromosome chr19 scaffold_4, whole genome shotgun
sequence - Vitis vinifera (Grape), partial (15%)
Length = 779
Score = 103 bits (52), Expect = 4e-21
Identities = 156/193 (80%)
Strand = Plus / Plus
Query: 1293 gaacttgataaaggggaatattcttctctgtggctattcatacaactttgtttctggtac 1352
|||||||||| |||||| | ||||||| ||||||||||| |||||||||||| |||||||
Sbjct: 288 gaacttgatagaggggattgttcttctttgtggctattcttacaactttgttcctggtac 347
Query: 1353 tgcatcaatnnnnnnngtctctgaaacagcaaaggcccttggagcagttggatttgttct 1412
||||| ||| |||||| |||||||||| |||||| ||||| | ||| ||||||
Sbjct: 348 tgcattaataaagaaagtctctaaaacagcaaaaacccttgaagcaggttgatatgttct 407
Query: 1413 ttgtgtagaaaatgtttctcctggaatgaaatttgatccagtccctgttggccttcctgg 1472
|| |||| |||||||| ||| || |||| | |||||||| | ||||||||||| ||
Sbjct: 408 ctgaatagagaatgtttcccctaaaaggaaactagatccagttcatgttggccttcttga 467
Query: 1473 gattgttatcaca 1485
||| ||||||||
Sbjct: 468 cattcttatcaca 480
Score = 97.6 bits (49), Expect = 3e-19
Identities = 68/73 (93%), Gaps = 1/73 (1%)
Strand = Plus / Plus
Query: 1181 ctacacgtttaaacaaa-acatacacgttggttgctgcaaatgatgttctgctagattcc 1239
|||||| |||||||||| |||||||| |||||||||||||||||||||||||||||||||
Sbjct: 200 ctacacatttaaacaaatacatacactttggttgctgcaaatgatgttctgctagattcc 259
Query: 1240 tcagttatgaagt 1252
|||||||||||
Sbjct: 260 caagttatgaagt 272
>gnl|LJGI|TC81905 similar to UniRef100_A7QXN3 Cluster: Chromosome undetermined
scaffold_226, whole genome shotgun sequence; n=1; Vitis
vinifera|Rep: Chromosome undetermined scaffold_226, whole
genome shotgun sequence - Vitis vinifera (Grape), partial
(4%)
Length = 1441
Score = 97.6 bits (49), Expect = 3e-19
Identities = 68/73 (93%), Gaps = 1/73 (1%)
Strand = Plus / Plus
Query: 1181 ctacacgtttaaacaaaa-catacacgttggttgctgcaaatgatgttctgctagattcc 1239
|||||||||||||||||| ||| ||| ||||||||||||| |||||||||||||||||||
Sbjct: 858 ctacacgtttaaacaaaaacatgcactttggttgctgcaagtgatgttctgctagattcc 917
Query: 1240 tcagttatgaagt 1252
||||||||||||
Sbjct: 918 ccagttatgaagt 930
Score = 60.0 bits (30), Expect = 6e-08
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 1386 ggcccttggagcagttggatttgttctttgtgtagaaaatgtttct 1431
|||||||||||||||| ||||| ||||||||||| | |||||||||
Sbjct: 959 ggcccttggagcagtttgattttttctttgtgtatagaatgtttct 1004
>gnl|LJGI|TC75277 similar to UniRef100_Q4U4M3 Cluster: Subtilisin-like protease; n=1;
Arachis hypogaea|Rep: Subtilisin-like protease - Arachis
hypogaea (Peanut), partial (9%)
Length = 1232
Score = 97.6 bits (49), Expect = 3e-19
Identities = 68/73 (93%), Gaps = 1/73 (1%)
Strand = Plus / Plus
Query: 1181 ctacacgtttaaacaaaa-catacacgttggttgctgcaaatgatgttctgctagattcc 1239
|||||||||||||||||| ||| ||| ||||||||||||| |||||||||||||||||||
Sbjct: 603 ctacacgtttaaacaaaaacatgcactttggttgctgcaagtgatgttctgctagattcc 662
Query: 1240 tcagttatgaagt 1252
||||||||||||
Sbjct: 663 ccagttatgaagt 675
Score = 63.9 bits (32), Expect = 4e-09
Identities = 44/48 (91%)
Strand = Plus / Plus
Query: 1384 aaggcccttggagcagttggatttgttctttgtgtagaaaatgtttct 1431
|||||||||||||||||| ||||| ||||||||||| | |||||||||
Sbjct: 707 aaggcccttggagcagtttgatttcttctttgtgtatagaatgtttct 754
>gnl|LJGI|TC65862 similar to UniRef100_Q4U4M3 Cluster: Subtilisin-like protease; n=1;
Arachis hypogaea|Rep: Subtilisin-like protease - Arachis
hypogaea (Peanut), partial (7%)
Length = 671
Score = 97.6 bits (49), Expect = 3e-19
Identities = 68/73 (93%), Gaps = 1/73 (1%)
Strand = Plus / Plus
Query: 1181 ctacacgtttaaacaaaa-catacacgttggttgctgcaaatgatgttctgctagattcc 1239
|||||||||||||||||| ||| ||| ||||||||||||| |||||||||||||||||||
Sbjct: 200 ctacacgtttaaacaaaaacatgcactttggttgctgcaagtgatgttctgctagattcc 259
Query: 1240 tcagttatgaagt 1252
||||||||||||
Sbjct: 260 ccagttatgaagt 272
Score = 60.0 bits (30), Expect = 6e-08
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 1386 ggcccttggagcagttggatttgttctttgtgtagaaaatgtttct 1431
|||||||||||||||| ||||| ||||||||||| | |||||||||
Sbjct: 301 ggcccttggagcagtttgatttcttctttgtgtatagaatgtttct 346