Miyakogusa Predicted Gene
- Lj6g3v0727750.2
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj6g3v0727750.2 Non Characterized Hit- tr|I1N087|I1N087_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.19659 PE,85.45,0,no
description,NULL; RCMTFAMILY,RNA (C5-cytosine) methyltransferase;
seg,NULL; Nol1_Nop2_Fmu,Bacteri,CUFF.58216.2
(276 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr3g460700.1 | NOL1/NOP2/sun family RNA methylase, putative |... 477 e-135
Medtr5g096020.1 | ribosomal RNA small subunit methyltransferase ... 114 8e-26
Medtr3g086950.1 | tRNA (cytosine(34)-C(5))-methyltransferase-lik... 87 2e-17
Medtr8g089980.1 | tRNA (cytosine(34)-C(5))-methyltransferase-lik... 84 1e-16
Medtr3g104760.1 | NOL1/NOP2/sun family RNA methylase, putative |... 84 2e-16
Medtr5g091110.1 | NOL1/NOP2/sun family protein | HC | chr5:39705... 64 2e-10
Medtr8g075430.1 | NOL1/NOP2/sun family RNA methylase, putative |... 50 3e-06
Medtr8g075430.2 | NOL1/NOP2/sun family RNA methylase, putative |... 49 4e-06
>Medtr3g460700.1 | NOL1/NOP2/sun family RNA methylase, putative | HC
| chr3:23817126-23809675 | 20130731
Length = 657
Score = 477 bits (1227), Expect = e-135, Method: Compositional matrix adjust.
Identities = 231/276 (83%), Positives = 246/276 (89%), Gaps = 1/276 (0%)
Query: 1 MAGFYMLQSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNTGIIFANEMKVPR 60
MAGFYMLQSASSFLPVMALAPQEKER+VDMAAAPGGKTTYIAALMKN+GIIFANEMKVPR
Sbjct: 289 MAGFYMLQSASSFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPR 348
Query: 61 LKSLTANLHRMGVSNTVVCNYDGKELPKVLGLNTVDRVLLDAPCSGTGVISKDESVKTSK 120
LKSLTANLHRMGVSNTVV NYDGKELPKVLG N+VDRVLLDAPCSGTGVISKDESVKTSK
Sbjct: 349 LKSLTANLHRMGVSNTVVSNYDGKELPKVLGFNSVDRVLLDAPCSGTGVISKDESVKTSK 408
Query: 121 DLEDIQKCAQLQKELILAAIDMVDANSKSGGYLVYSTCSIMVAENEAVIDYALKRRDVKL 180
+LEDI+KCA LQKEL+LAAIDMVD+ SKSGGY+VYSTCSIMVAENEAVIDY LKRRDVKL
Sbjct: 409 NLEDIKKCAHLQKELLLAAIDMVDSYSKSGGYVVYSTCSIMVAENEAVIDYVLKRRDVKL 468
Query: 181 VACGLNFGRPGFTKFREQRFHRSLDKTRRFYPHVHNMDGFFVAKLKKLSNSKQGAKPSET 240
V CGL+FGRPGFTKFREQRFH SLDKTRRFYPHVHNMDGFFVAKLKK+S+ K A+ SET
Sbjct: 469 VPCGLDFGRPGFTKFREQRFHPSLDKTRRFYPHVHNMDGFFVAKLKKMSHPK-PARTSET 527
Query: 241 SXXXXXXXXXXXXXDKPSNGVKENGKQTSESELKTK 276
DKPSNGV+ENGK + E E K +
Sbjct: 528 FEKDEKETELVIDDDKPSNGVEENGKHSFELESKKR 563
>Medtr5g096020.1 | ribosomal RNA small subunit methyltransferase B |
HC | chr5:41982400-41975357 | 20130731
Length = 529
Score = 114 bits (286), Expect = 8e-26, Method: Compositional matrix adjust.
Identities = 85/233 (36%), Positives = 123/233 (52%), Gaps = 33/233 (14%)
Query: 3 GFYMLQSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNTGIIFANEMKVPRLK 62
G +Q S+ L V + PQ E +VD AAPGGKT Y+AA + G+++A ++ RL+
Sbjct: 316 GLCSVQDESAGLIVSIVDPQPGETIVDCCAAPGGKTLYMAAHLSGQGMVYAVDVNSGRLR 375
Query: 63 SL--TANLHRM-GVSNTVVCNY----DGKELPKVLGLNTVDRVLLDAPCSGTGVISKDES 115
L TA LH++ GV TV + DG+ L ++VLLDAPCSG GV+SK
Sbjct: 376 ILKETAKLHQVDGVVTTVHADLRTLTDGEPLKS-------NKVLLDAPCSGLGVLSKRAD 428
Query: 116 VKTSKDLEDIQKCAQLQKELILAAIDMVDANSKSGGYLVYSTCSIMVAENEAVIDYALKR 175
++ ++ LED+++ +LQ EL+ AA +V K GG LVYSTCSI EN+ + L+R
Sbjct: 429 LRWNRKLEDMEQLKKLQDELLDAASTLV----KPGGVLVYSTCSIDPEENDDRVAAFLER 484
Query: 176 RDVKLVACGLNFGRPGFTKFREQRFHRSLDKTRRFY---PHVHNMDGFFVAKL 225
+ + P F + FY P H++DG F A+L
Sbjct: 485 HADFHIDPVDRYVPPDFV------------TPKGFYSSNPVKHSLDGSFAARL 525
>Medtr3g086950.1 | tRNA (cytosine(34)-C(5))-methyltransferase-like
protein | HC | chr3:39418089-39425597 | 20130731
Length = 784
Score = 87.0 bits (214), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 58/190 (30%), Positives = 89/190 (46%), Gaps = 26/190 (13%)
Query: 8 QSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNT--------GIIFANEMKVP 59
Q A S +P + L V+DM AAPG KT + ++ + G++ AN++ V
Sbjct: 159 QEAVSMVPPLFLDVHSNHVVLDMCAAPGSKTFQLLEILHRSTKAGSLPDGMVVANDLDVQ 218
Query: 60 RLKSLTANLHRMGVSNTVVCNYDGKELPK--------VLGLNT------VDRVLLDAPCS 105
R L + RM +N +V N++ + P+ ++G + DRVL D PCS
Sbjct: 219 RCNLLIHQIKRMCTANLIVTNHEAQHFPRCQLNMDCDIMGPDQHNSQLLFDRVLCDVPCS 278
Query: 106 GTGVISKDESVKTSKDLEDIQKCAQLQKELILAAIDMVDANSKSGGYLVYSTCSIMVAEN 165
G G + K + + LQ + + + ++ K GG +VYSTCSI EN
Sbjct: 279 GDGTLRKAPDLWRRWNTGTGNGLHNLQILVAMRGLSLL----KVGGRMVYSTCSINPIEN 334
Query: 166 EAVIDYALKR 175
EAVI L+R
Sbjct: 335 EAVIAEILRR 344
>Medtr8g089980.1 | tRNA (cytosine(34)-C(5))-methyltransferase-like
protein | HC | chr8:37616552-37624367 | 20130731
Length = 818
Score = 84.3 bits (207), Expect = 1e-16, Method: Compositional matrix adjust.
Identities = 66/217 (30%), Positives = 95/217 (43%), Gaps = 32/217 (14%)
Query: 8 QSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNT--------GIIFANEMKVP 59
Q A S +P + L V+DM AAPG KT + ++ + G++ AN++ V
Sbjct: 166 QEAVSMVPPLFLDVHSDHFVLDMCAAPGSKTFQLLEIIHQSTKAGSLPDGMVVANDLDVK 225
Query: 60 RLKSLTANLHRMGVSNTVVCN--------------YDGKELPKVLGLNTVDRVLLDAPCS 105
R L RM +N +V N YD EL + DRVL D PCS
Sbjct: 226 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRLKGNYDRMELDHNIHQLLFDRVLCDVPCS 285
Query: 106 GTGVISKDESVKTSKDLEDIQKCAQLQKELILAAIDMVDANSKSGGYLVYSTCSIMVAEN 165
G G + K + + Q LQ + + + ++ K GG +VYSTCS+ EN
Sbjct: 286 GDGTLRKAPDMWRKWNSGLGQGLHSLQVLIAMRGLSLL----KIGGRMVYSTCSMNPIEN 341
Query: 166 EAVIDYALKR--RDVKLVACGLNFG----RPGFTKFR 196
EAV+ L+R VKLV RPG +++
Sbjct: 342 EAVVAEVLRRCGESVKLVDVSSELPQLIRRPGLKRWK 378
>Medtr3g104760.1 | NOL1/NOP2/sun family RNA methylase, putative | HC
| chr3:48292253-48297828 | 20130731
Length = 509
Score = 83.6 bits (205), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 64/179 (35%), Positives = 91/179 (50%), Gaps = 13/179 (7%)
Query: 3 GFYMLQSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNTGIIFANEMKVPRLK 62
G LQ +S + AL+P+ V+D AAPG KT ++AALMK G I A E+K R+K
Sbjct: 224 GSIFLQGKASSMVAPALSPEPGWEVLDACAAPGNKTVHLAALMKRKGRIIACELKKERIK 283
Query: 63 SLTANLHRMGVSNTVVCNYDGKEL-PKVLGLNTVDRVLLDAPCSGTGV-ISKDESVKTSK 120
L + G +N V N D + K + V +LLD CSG+G S+ + + SK
Sbjct: 284 RLNDTIKLSGATNIQVLNEDFLNINSKDPAYSKVKAILLDPSCSGSGTAASRLDHLLPSK 343
Query: 121 ------DLEDIQKCAQLQKELILAAIDMVDANSKSGGYLVYSTCSIMVAENEAVIDYAL 173
D+E + K A Q++ + A + A + +VYSTCSI ENE V+ L
Sbjct: 344 AAGQNVDMERLNKLAAFQRKALQHAF-LFPALER----VVYSTCSINQIENEDVVKSVL 397
>Medtr5g091110.1 | NOL1/NOP2/sun family protein | HC |
chr5:39705460-39695039 | 20130731
Length = 606
Score = 63.5 bits (153), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 47/143 (32%), Positives = 71/143 (49%), Gaps = 16/143 (11%)
Query: 93 NTVDRVLLDAPCSGTGVISKDESVKTSKDLEDIQKCAQLQKELILAAIDMVDANSKSGGY 152
++ DRVLLDAPCS G+ + +E ++ A+ Q+ + A+ +V + GG
Sbjct: 463 DSFDRVLLDAPCSALGL--RPRLFAGEDTIESLRSHAKYQRRMFDQAVQLV----RPGGV 516
Query: 153 LVYSTCSIMVAENEAVIDYAL-KRRDVKLVACGLNFGRPGFT---KFREQRFHRSL---- 204
+VYSTC+I ENEA++ YAL K + + L G PG +F + L
Sbjct: 517 IVYSTCTINPGENEALVRYALDKYKYLSLAPQHPKIGGPGLVGSCEFPDGYAEEWLRPGE 576
Query: 205 -DKTRRFYPHVH-NMDGFFVAKL 225
D +RF P + GFF+AK
Sbjct: 577 EDLVQRFDPSSPLDTIGFFIAKF 599
Score = 56.6 bits (135), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 32/71 (45%), Positives = 42/71 (59%), Gaps = 3/71 (4%)
Query: 1 MAGFYMLQSASSFLPVMALAPQEKERVVDMAAAPGGKTTYIAALMKNTGIIFANEM---K 57
+ G LQ+ S + AL PQ ER++DM AAPGGKTT IA LMK+ G + A + K
Sbjct: 284 LEGEIFLQNLPSIIAAHALDPQMGERILDMCAAPGGKTTAIAILMKDKGEVIATDRSHNK 343
Query: 58 VPRLKSLTANL 68
V ++ L A L
Sbjct: 344 VLDIEKLAAEL 354
>Medtr8g075430.1 | NOL1/NOP2/sun family RNA methylase, putative | HC
| chr8:31899500-31903095 | 20130731
Length = 389
Score = 49.7 bits (117), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 43/145 (29%), Positives = 62/145 (42%), Gaps = 26/145 (17%)
Query: 96 DRVLLDAPCSGTGVISKDESVKTSK---------DLEDIQKCAQLQKELILAAIDMVDAN 146
D+VL+DA C+ G I + + D E LQ L+ ++
Sbjct: 257 DKVLVDAECTHDGSIKHIQKFEQWGWITLQRRVLDAERTDDLHALQLNLLTNGFRLL--- 313
Query: 147 SKSGGYLVYSTCSIMVAENEAVIDYALKRR---DVKLVACGLNFGRPGFTKFREQRFHRS 203
K GG LVYSTCS+ VA+NE V++ LK ++ + N+ G
Sbjct: 314 -KVGGSLVYSTCSLTVAQNEDVMEQFLKENITAELTEIDAARNWPCKG----------GR 362
Query: 204 LDKTRRFYPHVHNMDGFFVAKLKKL 228
+ KT RF P G FVAK +K+
Sbjct: 363 IRKTWRFDPLTSQTSGLFVAKFRKV 387
>Medtr8g075430.2 | NOL1/NOP2/sun family RNA methylase, putative | HC
| chr8:31899500-31903095 | 20130731
Length = 290
Score = 49.3 bits (116), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 44/142 (30%), Positives = 62/142 (43%), Gaps = 20/142 (14%)
Query: 96 DRVLLDAPCSGTGVISKDESVKTSK---------DLEDIQKCAQLQKELILAAIDMVDAN 146
D+VL+DA C+ G I + + D E LQ L+ ++
Sbjct: 158 DKVLVDAECTHDGSIKHIQKFEQWGWITLQRRVLDAERTDDLHALQLNLLTNGFRLL--- 214
Query: 147 SKSGGYLVYSTCSIMVAENEAVIDYALKRRDVKLVACGLNFGRPGFTKFREQRFHRSLDK 206
K GG LVYSTCS+ VA+NE V++ LK ++ ++ R K R K
Sbjct: 215 -KVGGSLVYSTCSLTVAQNEDVMEQFLK-ENITAELTEIDAARNWPCKGGRIR------K 266
Query: 207 TRRFYPHVHNMDGFFVAKLKKL 228
T RF P G FVAK +K+
Sbjct: 267 TWRFDPLTSQTSGLFVAKFRKV 288