Miyakogusa Predicted Gene
- Lj5g3v0290420.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj5g3v0290420.1 tr|G7I3K2|G7I3K2_MEDTR Alpha-amylase OS=Medicago
truncatula GN=MTR_1g019440 PE=3 SV=1,87.17,0,Alpha-amylase
domain,Glycosyl hydrolase, family 13, subfamily, catalytic domain;
Alpha-amylase C-ter,CUFF.52722.1
(421 letters)
Database: Glyma1.pep
75,778 sequences; 25,431,882 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Glyma17g36090.1 705 0.0
Glyma08g26860.1 387 e-107
Glyma08g40810.1 384 e-106
Glyma14g39930.1 381 e-106
Glyma02g02450.1 375 e-104
Glyma17g38100.1 149 8e-36
Glyma14g09090.1 115 9e-26
Glyma05g06180.1 81 2e-15
Glyma18g16250.1 79 1e-14
>Glyma17g36090.1
Length = 414
Score = 705 bits (1820), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 336/394 (85%), Positives = 363/394 (92%), Gaps = 7/394 (1%)
Query: 28 QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
QGFNWESS KGGWYNSLKN+IPDLANAGITHVWLPPPSQSV+ +GYLPGRLYDLDASKYG
Sbjct: 28 QGFNWESSKKGGWYNSLKNTIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYG 87
Query: 88 SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
+KD+LKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDA LDWGPSFIC+
Sbjct: 88 TKDQLKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPSFICK 147
Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
DD YSDG GNLDSGE YD APDIDHLNPQVQRELS+WMNWL+ E+GFDGWRFD+VKGYA
Sbjct: 148 DDNTYSDGTGNLDSGEPYDPAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDYVKGYA 207
Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
PSITKIYME+T PDFAVGE W+SLS N D HRG LVNWV+SAGGA+ AFDFT
Sbjct: 208 PSITKIYMEQTRPDFAVGEKWDSLSID-------NYDGHRGALVNWVESAGGAITAFDFT 260
Query: 268 TKGILQAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQG 327
TKGILQAAVQG+LWRLKDSNGKP GMIGVKPENAVTF+DNHDTGSTQ++WPFPSDKVMQG
Sbjct: 261 TKGILQAAVQGQLWRLKDSNGKPSGMIGVKPENAVTFIDNHDTGSTQRIWPFPSDKVMQG 320
Query: 328 YAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEID 387
YAYILTHPGTP+IFYDHFFDWGLKEQIAKLS+IRV+ GIN KS+VNILAA++DLYVA+ID
Sbjct: 321 YAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRVKHGINEKSSVNILAAEADLYVAKID 380
Query: 388 NKIIVKIGPKMDLGNLIPSSFRVAASGQDYAVWE 421
NKI +KIGPKMDLGNLIP +F VA SGQDYAVWE
Sbjct: 381 NKIFLKIGPKMDLGNLIPPNFHVATSGQDYAVWE 414
>Glyma08g26860.1
Length = 481
Score = 387 bits (994), Expect = e-107, Method: Compositional matrix adjust.
Identities = 191/398 (47%), Positives = 269/398 (67%), Gaps = 15/398 (3%)
Query: 28 QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
QGFNWES + WY L DL+N G+T VWLPPP++SVA QGY+P LY+L++S YG
Sbjct: 91 QGFNWES-WRRRWYLELAAKTADLSNCGVTAVWLPPPTESVAPQGYMPSDLYNLNSS-YG 148
Query: 88 SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
S +ELK I H + + L D+V+NHR A+++ G++ IF G L WGP I
Sbjct: 149 SVEELKYCIEEMHSQDLLALGDVVLNHRCAQKQSPNGVWNIFGG-----KLAWGPEAIVC 203
Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
DD + G+GN SG+ + AAP++DH V++++ +W+NWLRN++GFDGWR DFV+G++
Sbjct: 204 DDPNFQ-GRGNPSSGDIFHAAPNVDHSQDFVRKDIKEWLNWLRNDIGFDGWRLDFVRGFS 262
Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
+ K Y+E ++P FA+GEYW+SL Y + G +NQDAHR ++NW+ + GG +AFD T
Sbjct: 263 GTYVKEYIEASTPVFAIGEYWDSLGY-EHGSLCYNQDAHRQRIINWINATGGTSSAFDMT 321
Query: 268 TKGILQAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQG 327
TKGIL +A+ E WRL D GKP G++G AVTF++NHDTGSTQ WPFP DK+MQG
Sbjct: 322 TKGILHSALHNEYWRLIDPQGKPTGVMGWWASRAVTFLENHDTGSTQGHWPFPRDKLMQG 381
Query: 328 YAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEID 387
YAYILTHPGTPTIFYDHF+D+G+ + + +L R R GI+ +S++ I A+++ YVA++
Sbjct: 382 YAYILTHPGTPTIFYDHFYDFGIHDVLTELIDARRRAGIHCRSSIKIYHANNEGYVAQVG 441
Query: 388 NKIIVKIG-----PKMDLGNLIPSSFRVAASGQDYAVW 420
+ +++K+G P + L S + G DY VW
Sbjct: 442 DALVMKLGQFDWNPSKE-NQLEGSWQKFVDKGPDYQVW 478
>Glyma08g40810.1
Length = 911
Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust.
Identities = 195/399 (48%), Positives = 263/399 (65%), Gaps = 18/399 (4%)
Query: 28 QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
QGFNWES G WY LK +LA+ G T +WLPPP++SV+ +GY+P LY+L+ S+YG
Sbjct: 524 QGFNWESHKSGRWYMELKEKAAELASFGFTVIWLPPPTESVSPEGYMPKDLYNLN-SRYG 582
Query: 88 SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
+ DELK ++ H+ GIK L D V+NHR A K+ GI+ +F G L+W I
Sbjct: 583 TIDELKDVVKTLHEVGIKVLGDAVLNHRCAHFKNQSGIWNLFGG-----RLNWDDRAIVA 637
Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
DD + G+GN SG+ + AAP+IDH V+++L +W+ W+R E+G+DGWR DFV+G+
Sbjct: 638 DDPHFQ-GRGNKSSGDNFHAAPNIDHSQDFVRKDLKEWLCWMREEIGYDGWRLDFVRGFW 696
Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
K Y+E + P FAVGEYW+SLSY G+ + NQDAHR +V+W+ + G AFD T
Sbjct: 697 GGYVKDYLEASEPYFAVGEYWDSLSYTY-GEMDHNQDAHRQRIVDWINATAGTAGAFDVT 755
Query: 268 TKGILQAAVQ-GELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQ 326
TKGIL +A++ E WRL D GKPPG++G P AVTF++NHDTGSTQ W FPS K MQ
Sbjct: 756 TKGILHSALERCEYWRLSDQKGKPPGVLGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQ 815
Query: 327 GYAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEI 386
GYAY LTHPGTP++FYDH F K +IA L +IR R I+ +ST+ I A+ D+Y A +
Sbjct: 816 GYAYTLTHPGTPSVFYDHIFS-HYKTEIATLISIRKRNKIHCRSTLKICKAERDVYAAIV 874
Query: 387 DNKIIVKIGPKMDLGNLIPSS----FRVAASGQDYAVWE 421
D+K+ +KIGP G+ P S + A G+DY +WE
Sbjct: 875 DDKVAMKIGP----GHFEPPSGSQRWSSALEGRDYKIWE 909
>Glyma14g39930.1
Length = 413
Score = 381 bits (979), Expect = e-106, Method: Compositional matrix adjust.
Identities = 194/398 (48%), Positives = 259/398 (65%), Gaps = 19/398 (4%)
Query: 28 QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
Q FNWES+ K W+N+L+ + D+A AG T VWLPPP+ S + +GY P LY L+ SKYG
Sbjct: 30 QAFNWESN-KYNWWNNLEGKVSDIAKAGFTSVWLPPPTHSFSPEGYTPQNLYSLN-SKYG 87
Query: 88 SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
S+ +LK+L+ ++ +ADIVINHRT + G+Y F+G L W +
Sbjct: 88 SERQLKALLQKMKQYKVRAMADIVINHRTGTTQGRGGMYNRFDG----IPLGWDERAVTS 143
Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
D S G GN +G + P+IDH V++++ W+ WLR+EVGF +RFDFVKG++
Sbjct: 144 D----SGGLGNRSTGAIFQGFPNIDHTQDFVRKDIIGWLRWLRHEVGFQDFRFDFVKGFS 199
Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
P K Y+E P F VGEYW+S +Y + ++NQD+HR ++NW+ G AFDFT
Sbjct: 200 PKYVKEYIEGAKPLFCVGEYWDSCNY-KGSTLDYNQDSHRQRIINWIDGTGQLSTAFDFT 258
Query: 268 TKGILQAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQG 327
TKGILQ AV+G WRL+D GKPPG+IG P +VTFVDNHDTGSTQ WPFP D +M+G
Sbjct: 259 TKGILQEAVKGNFWRLRDPQGKPPGVIGWWPSRSVTFVDNHDTGSTQAHWPFPKDHIMEG 318
Query: 328 YAYILTHPGTPTIFYDHFFDWG--LKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAE 385
YAYILTHPG PT+FYDHF+DWG ++EQI KL +R R+GI +S+V IL A DLY A
Sbjct: 319 YAYILTHPGIPTVFYDHFYDWGDSIREQIVKLIDVRKRQGIQSRSSVRILEAKHDLYSAV 378
Query: 386 IDNKIIVKIGPKMDLGNLIPS--SFRVAASGQDYAVWE 421
I K+ +KIG G+ P+ + ++ SG +YAVW
Sbjct: 379 IGEKVCMKIGN----GSWCPTGREWTLSTSGHNYAVWH 412
>Glyma02g02450.1
Length = 881
Score = 375 bits (963), Expect = e-104, Method: Compositional matrix adjust.
Identities = 194/399 (48%), Positives = 260/399 (65%), Gaps = 18/399 (4%)
Query: 28 QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
Q FNWES G WY LK +LA+ G T VWLPPP++SV+ +GY+P LY+L+ S+YG
Sbjct: 494 QAFNWESHKSGRWYIELKEMASELASLGFTVVWLPPPTESVSPEGYMPKDLYNLN-SRYG 552
Query: 88 SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
+ DELK L+ FH+ GIK L D V+NHR A ++ GI+ IF G L+W +
Sbjct: 553 NIDELKDLVKRFHEVGIKVLGDAVLNHRCAHYQNQNGIWNIFGG-----PLNWDDRAVVA 607
Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
DD + G+GN SG+ + AAP+IDH V+++L +W+ WLR EVG+DGWR DFV+G+
Sbjct: 608 DDPHFQ-GRGNKSSGDNFHAAPNIDHSQEFVRKDLKEWLCWLRKEVGYDGWRLDFVRGFW 666
Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
K Y++ + P F+VGEYW+SLSY + + NQDAHR +++W+ + G AFD T
Sbjct: 667 GGYVKDYIDASEPYFSVGEYWDSLSYTY-SEMDHNQDAHRQRIIDWINATNGTSGAFDVT 725
Query: 268 TKGILQAAVQ-GELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQ 326
TKGIL A++ E WRL D GKPPG++G P AVTF++NHDTGSTQ W FPS K MQ
Sbjct: 726 TKGILHPALERCEYWRLSDEKGKPPGVLGWWPSRAVTFIENHDTGSTQGHWRFPSGKQMQ 785
Query: 327 GYAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEI 386
GYAYILTHPGTP++FYDH K +IA L ++R R I+ +S V I A+ D+Y A I
Sbjct: 786 GYAYILTHPGTPSVFYDHISSHD-KSEIASLISLRKRNKIHCRSRVQISKAEKDVYAAII 844
Query: 387 DNKIIVKIGPKMDLGNLIPSS----FRVAASGQDYAVWE 421
D K+ +KIGP G+ P S + +A G+DY +WE
Sbjct: 845 DEKVAMKIGP----GHFEPPSDSQKWSLAIEGKDYKIWE 879
>Glyma17g38100.1
Length = 249
Score = 149 bits (375), Expect = 8e-36, Method: Compositional matrix adjust.
Identities = 76/184 (41%), Positives = 109/184 (59%), Gaps = 6/184 (3%)
Query: 146 CRDDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKG 205
CR S KGN +G+ + P+IDH V++++ W+ WLR+EVGF +RF FVKG
Sbjct: 10 CRHQLTPSHLKGNRSTGDIFHGFPNIDHTKDFVRKDIIGWLRWLRHEVGFHDFRFGFVKG 69
Query: 206 YAPSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFD 265
++P K Y+E P F VGEYW+S +Y + ++NQ E+ ++
Sbjct: 70 FSPKYVKEYIEGAKPLFCVGEYWHSCNY-KGSTLDYNQAL--CEVCDFQYLHFVLCTLII 126
Query: 266 FTTKGILQA---AVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSD 322
+ ++ + A AV+G+ WRL D GKPPG+IG P +VTFVD+HDTGSTQ WPFP D
Sbjct: 127 YVSECLKNAHGKAVKGDFWRLCDPQGKPPGVIGWWPSRSVTFVDDHDTGSTQAHWPFPKD 186
Query: 323 KVMQ 326
+M+
Sbjct: 187 HIME 190
>Glyma14g09090.1
Length = 90
Score = 115 bits (288), Expect = 9e-26, Method: Composition-based stats.
Identities = 55/89 (61%), Positives = 67/89 (75%)
Query: 330 YILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEIDNK 389
YI+T FYD + +WGLKE I K++ IR R G+ S VNILAA++DLY+AEIDNK
Sbjct: 2 YIVTTMSMCMQFYDPYIEWGLKEPIKKVTEIRKRNGVTATSRVNILAAEADLYMAEIDNK 61
Query: 390 IIVKIGPKMDLGNLIPSSFRVAASGQDYA 418
IIVKIGPKMDL NL+P + +VA SGQDYA
Sbjct: 62 IIVKIGPKMDLVNLLPPNVQVATSGQDYA 90
>Glyma05g06180.1
Length = 138
Score = 81.3 bits (199), Expect = 2e-15, Method: Composition-based stats.
Identities = 38/65 (58%), Positives = 43/65 (66%), Gaps = 4/65 (6%)
Query: 273 QAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQGYAYIL 332
+A+ E WRL D GKP G++G A DNHDTGSTQ WPFP DK+MQGYAYIL
Sbjct: 78 HSALHNEYWRLIDPQGKPTGVMGWWASCA----DNHDTGSTQGHWPFPRDKLMQGYAYIL 133
Query: 333 THPGT 337
HP T
Sbjct: 134 IHPRT 138
>Glyma18g16250.1
Length = 604
Score = 78.6 bits (192), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 63/201 (31%), Positives = 87/201 (43%), Gaps = 48/201 (23%)
Query: 103 GIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICRDDTAYSDGK------ 156
GIK L V+NHR A K+ GI+ IF G ++W I DD+ +
Sbjct: 364 GIKVLGYAVLNHRCAHFKNQNGIWNIFGG-----HVNWDDRAIVADDSYFQFNSLLVKEL 418
Query: 157 -------------GNLDSGEGYDAAPDIDHLNPQV----------QRELSDWMNWLRN-- 191
GNL P++ P V R L L+
Sbjct: 419 RDTLQIVYFMFMFGNLTIL--LSKQPNMKLTTPFVVIFLPCRGGATRVLETIFMLLQTLI 476
Query: 192 ------EVGFDGWRFDFVKGYAPSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDA 245
E+G+DGWR DFV+G+ K Y+E + P FAVGEYW+SL Y G+ + NQDA
Sbjct: 477 IHRTLEEIGYDGWRLDFVRGFWGGYVKDYLEASEPYFAVGEYWDSLGYTY-GEMDHNQDA 535
Query: 246 HRGELVNWVQSAGGAVNAFDF 266
H +V+W+ + +AFD
Sbjct: 536 HGQRIVDWINATA---DAFDL 553