Miyakogusa Predicted Gene

Lj5g3v0290420.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj5g3v0290420.1 tr|G7I3K2|G7I3K2_MEDTR Alpha-amylase OS=Medicago
truncatula GN=MTR_1g019440 PE=3 SV=1,87.17,0,Alpha-amylase
domain,Glycosyl hydrolase, family 13, subfamily, catalytic domain;
Alpha-amylase C-ter,CUFF.52722.1
         (421 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G25000.1 | Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:...   553   e-158
AT1G69830.1 | Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr...   377   e-105
AT1G76130.1 | Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr...   364   e-101

>AT4G25000.1 | Symbols: ATAMY1, AMY1 | alpha-amylase-like |
           chr4:12852109-12853825 REVERSE LENGTH=423
          Length = 423

 Score =  553 bits (1426), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 267/396 (67%), Positives = 322/396 (81%), Gaps = 6/396 (1%)

Query: 28  QGFNWESSGK-GGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKY 86
           Q FNWES  K GG+YNSL NSI D+ANAGITH+WLPPPSQSVA +GYLPG+LYDL++SKY
Sbjct: 30  QSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNSSKY 89

Query: 87  GSKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFIC 146
           GS+ ELKSLI A + KGIK LADIVINHRTAERKD +  YC FEGGT D  LDW PSF+C
Sbjct: 90  GSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFVC 149

Query: 147 RDDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGY 206
           R+D  +  G GNLD+G  +D APDIDHLNP+VQ+ELS+WMNWL+ E+GF GWRFD+V+GY
Sbjct: 150 RNDPKFP-GTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVRGY 208

Query: 207 APSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAV-NAFD 265
           A SITK+Y++ TSPDFAVGE W+ + YG DGK +++Q+ HR  L  W++ AGG V  AFD
Sbjct: 209 ASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFD 268

Query: 266 FTTKGILQAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVM 325
           FTTKGILQ+AV+GELWRLKDS GKPPGMIG+ P NAVTF+DNHDT  T   W FPSDKV+
Sbjct: 269 FTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDKVL 325

Query: 326 QGYAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAE 385
            GY YILTHPGTP IFY+H+ +WGLKE I+KL AIR + GI   S+V I AA++DLY+A 
Sbjct: 326 LGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLAM 385

Query: 386 IDNKIIVKIGPKMDLGNLIPSSFRVAASGQDYAVWE 421
           ID+K+I+KIGPK D+G L+PS+F +A SG D+AVWE
Sbjct: 386 IDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421


>AT1G69830.1 | Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 |
           chr1:26288518-26293003 REVERSE LENGTH=887
          Length = 887

 Score =  377 bits (969), Expect = e-105,   Method: Compositional matrix adjust.
 Identities = 192/399 (48%), Positives = 262/399 (65%), Gaps = 18/399 (4%)

Query: 28  QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
           QGFNWES+  G WY  L+    +LA+ G T +WLPPP++SV+ +GY+P  LY+L+ S+YG
Sbjct: 500 QGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN-SRYG 558

Query: 88  SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
           + DELK  +  FH  GIK L D V+NHR A  K+  G++ +F G      L+W    +  
Sbjct: 559 TIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNWDDRAVVA 613

Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
           DD  +  G+GN  SG+ + AAP+IDH    V++++ +W+ W+  EVG+DGWR DFV+G+ 
Sbjct: 614 DDPHFQ-GRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFW 672

Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
               K YM+ + P FAVGEYW+SLSY   G+ ++NQDAHR  +V+W+ +  GA  AFD T
Sbjct: 673 GGYVKDYMDASKPYFAVGEYWDSLSYTY-GEMDYNQDAHRQRIVDWINATSGAAGAFDVT 731

Query: 268 TKGILQAAVQG-ELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQ 326
           TKGIL  A+Q  E WRL D  GKPPG++G  P  AVTF++NHDTGSTQ  W FP  K MQ
Sbjct: 732 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 791

Query: 327 GYAYILTHPGTPTIFYDHFFDWGLKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAEI 386
           GYAYILTHPGTP +F+DH F      +IA L ++R R+ ++ +S VNI  ++ D+Y A I
Sbjct: 792 GYAYILTHPGTPAVFFDHIFS-DYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAII 850

Query: 387 DNKIIVKIGPKMDLGNLIP----SSFRVAASGQDYAVWE 421
           D K+ +KIGP    G+  P     ++ VA  G+DY VWE
Sbjct: 851 DEKVAMKIGP----GHYEPPNGSQNWSVAVEGRDYKVWE 885


>AT1G76130.1 | Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 |
           chr1:28561647-28563914 FORWARD LENGTH=413
          Length = 413

 Score =  364 bits (935), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 187/398 (46%), Positives = 258/398 (64%), Gaps = 19/398 (4%)

Query: 28  QGFNWESSGKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVASQGYLPGRLYDLDASKYG 87
           Q +NWES  K  W+ +L   +PD+A +G T  WLPPPSQS+A +GYLP  LY L+ S YG
Sbjct: 30  QAYNWESH-KYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-SAYG 87

Query: 88  SKDELKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDASLDWGPSFICR 147
           S+  LKSL+       ++ +ADIVINHR    +   G+Y  ++G     SL W    +  
Sbjct: 88  SEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHAV-- 141

Query: 148 DDTAYSDGKGNLDSGEGYDAAPDIDHLNPQVQRELSDWMNWLRNEVGFDGWRFDFVKGYA 207
             T+ + G GN  +G+ ++  P++DH    V++++  W+ WLRN VGF  +RFDF +GY+
Sbjct: 142 --TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 199

Query: 208 PSITKIYMEKTSPDFAVGEYWNSLSYGQDGKPNFNQDAHRGELVNWVQSAGGAVNAFDFT 267
            +  K Y+    P F+VGE W+S +Y   G  ++NQD+HR  +++W+ + G    AFDFT
Sbjct: 200 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRIISWIDATGQISAAFDFT 258

Query: 268 TKGILQAAVQGELWRLKDSNGKPPGMIGVKPENAVTFVDNHDTGSTQKLWPFPSDKVMQG 327
           TKGILQ AV+G+ WRL D+ GKPPG++G  P  AVTF+DNHDTGSTQ  WPFPS  VM+G
Sbjct: 259 TKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 318

Query: 328 YAYILTHPGTPTIFYDHFFDWG--LKEQIAKLSAIRVRKGINMKSTVNILAADSDLYVAE 385
           YAYILTHPG P +FYDHF+DWG  + +QI KL  IR R+ I+ +STV +L A+S+LY A 
Sbjct: 319 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 378

Query: 386 IDNKIIVKIGPKMDLGNLIPSS--FRVAASGQDYAVWE 421
           +  KI +K+G     G+  PS   + +A SG  YAVW 
Sbjct: 379 VGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWH 412