Miyakogusa Predicted Gene
- Lj4g3v2860650.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj4g3v2860650.1 tr|G7LGJ1|G7LGJ1_MEDTR Glutamyl-tRNA(Gln)
amidotransferase subunit A OS=Medicago truncatula
GN=MTR_8,74.34,0,AMIDASE-RELATED,NULL; AMIDASE,Amidase; no
description,Amidase signature domain; seg,NULL;
Amidase,Am,CUFF.51788.1
(629 letters)
Database: TAIR10_pep
35,386 sequences; 14,482,855 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G07360.1 | Symbols: | Amidase family protein | chr5:2326925-... 835 0.0
AT5G07360.2 | Symbols: | Amidase family protein | chr5:2326925-... 818 0.0
AT3G25660.1 | Symbols: | Amidase family protein | chr3:9339640-... 105 1e-22
AT4G34880.1 | Symbols: | Amidase family protein | chr4:16615549... 84 2e-16
AT1G08980.1 | Symbols: ATAMI1, AMI1, ATTOC64-I, TOC64-I | amidas... 68 2e-11
AT5G64440.1 | Symbols: AtFAAH, FAAH | fatty acid amide hydrolase... 67 3e-11
AT5G09420.1 | Symbols: ATTOC64-V, MTOM64, TOC64-V, OM64, AtmtOM6... 60 7e-09
AT3G17970.1 | Symbols: atToc64-III, TOC64-III | translocon at th... 54 3e-07
>AT5G07360.1 | Symbols: | Amidase family protein |
chr5:2326925-2330011 REVERSE LENGTH=659
Length = 659
Score = 835 bits (2158), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 375/537 (69%), Positives = 457/537 (85%)
Query: 89 LLDANFFKDKKIEEITKGVSEMNIPIIKANRETVASENGGLHHPSPLVFNADWEHRLDHG 148
L+D+ FF + K++EI KG +EMN+PI +ANR+ VA++NGGL +PSPLVFN W +
Sbjct: 119 LIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRV 178
Query: 149 ATKRFSYPSISGIQRPESEEDIAFMSVLELGELLQTKQITSQELTQIFLKRLKKYNPILE 208
KRF YPS SG++ P EEDIAFMSVLELGEL++T+QITS+EL +I+LK+LK+YN +LE
Sbjct: 179 QDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLE 238
Query: 209 AVITYTEELAHNQAKEADKLLSQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKTFKNQVI 268
AV+TYTEELA+ QAKEAD LLSQG YLGPLHGIPYGLKDI++VPGYKTTWGS +FK+Q +
Sbjct: 239 AVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFL 298
Query: 269 NMEAWVYKRLKSAGAVLVAKLVSGSLAYDDIWFGGRTRNPWNIEEFXXXXXXXXXXXXXX 328
++EAWVYKRLK++GAVLVAKLV+GS+AYDDIWFGGRTRNPWNIEEF
Sbjct: 299 DIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSA 358
Query: 329 XLVPFAIGSETAGSITYPAARCGVTALRPTFGTVGRTGVMSLSESLDKLGPFCRSATDCA 388
+VPFAIGSETAGS+TYPAARCG+TALRPTFG+VGRTGVMS+SESLDKLGPFCR+A DCA
Sbjct: 359 GMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCA 418
Query: 389 VVLDIVRGRDPNDLSSRHSSLDDPFHVDITKLTVGYLDDAEMEVVNVLASKGVKMVPFKL 448
V+LD ++G+DP+DLSSR + +DPF VDITKLTVGY DA+M+VV VL SKGV MVPF+L
Sbjct: 419 VILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVGYTKDADMKVVEVLGSKGVNMVPFEL 478
Query: 449 NYTVDSAQGILNFTMDVDMLAHFDQWQRSGEDNVYEAQEQWPTELGRARLIPAVDYVQAQ 508
NYTV+S QGILNFTMDVDMLAHFD+WQR+G++++YEAQ+QWP EL RAR++ AVDY+QAQ
Sbjct: 479 NYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLYEAQDQWPVELRRARVVTAVDYIQAQ 538
Query: 509 RARGRLIKEIRESFSVDAFIGNATDWEKVCIGNLVGLPVMVVPTGFTNISDAPSGGSRRK 568
RARG+LI+E+ +SF+VDAFIGN TDWEKVC+GNLVGLPV+V+PTGF NI+D P+ RR+
Sbjct: 539 RARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFKNITDPPTNSCRRR 598
Query: 569 TTVTTGVYAPPNRDHIALALAMAYQEVTNHHKQRPPINDLGPNDKIPNPTNVTYPAR 625
TT+ G+YAPP RDHIALAL MAYQ VT+ H++RPPI+DLGP+D IPNP P R
Sbjct: 599 TTINAGIYAPPERDHIALALGMAYQSVTDAHRKRPPIDDLGPDDSIPNPPRALIPPR 655
>AT5G07360.2 | Symbols: | Amidase family protein |
chr5:2326925-2330011 REVERSE LENGTH=652
Length = 652
Score = 818 bits (2114), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 370/537 (68%), Positives = 452/537 (84%), Gaps = 7/537 (1%)
Query: 89 LLDANFFKDKKIEEITKGVSEMNIPIIKANRETVASENGGLHHPSPLVFNADWEHRLDHG 148
L+D+ FF + K++EI KG +EMN+PI +ANR+ VA++NGGL +PSPLVFN W +
Sbjct: 119 LIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPLVFNPSWNREVQRV 178
Query: 149 ATKRFSYPSISGIQRPESEEDIAFMSVLELGELLQTKQITSQELTQIFLKRLKKYNPILE 208
KRF YPS SG++ P EEDIAFMSVLELGEL++T+QITS+EL +I+LK+LK+YN +LE
Sbjct: 179 QDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLE 238
Query: 209 AVITYTEELAHNQAKEADKLLSQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKTFKNQVI 268
AV+TYTEELA+ QAKEAD LLSQG YLGPLHGIPYGLKDI++VPGYKTTWGS +FK+Q +
Sbjct: 239 AVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYKTTWGSTSFKDQFL 298
Query: 269 NMEAWVYKRLKSAGAVLVAKLVSGSLAYDDIWFGGRTRNPWNIEEFXXXXXXXXXXXXXX 328
++EAWVYKRLK++GAVLVAKLV+GS+AYDDIWFGGRTRNPWNIEEF
Sbjct: 299 DIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAAST-- 356
Query: 329 XLVPFAIGSETAGSITYPAARCGVTALRPTFGTVGRTGVMSLSESLDKLGPFCRSATDCA 388
+ GSETAGS+TYPAARCG+TALRPTFG+VGRTGVMS+SESLDKLGPFCR+A DCA
Sbjct: 357 -----SAGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCA 411
Query: 389 VVLDIVRGRDPNDLSSRHSSLDDPFHVDITKLTVGYLDDAEMEVVNVLASKGVKMVPFKL 448
V+LD ++G+DP+DLSSR + +DPF VDITKLTVGY DA+M+VV VL SKGV MVPF+L
Sbjct: 412 VILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVGYTKDADMKVVEVLGSKGVNMVPFEL 471
Query: 449 NYTVDSAQGILNFTMDVDMLAHFDQWQRSGEDNVYEAQEQWPTELGRARLIPAVDYVQAQ 508
NYTV+S QGILNFTMDVDMLAHFD+WQR+G++++YEAQ+QWP EL RAR++ AVDY+QAQ
Sbjct: 472 NYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLYEAQDQWPVELRRARVVTAVDYIQAQ 531
Query: 509 RARGRLIKEIRESFSVDAFIGNATDWEKVCIGNLVGLPVMVVPTGFTNISDAPSGGSRRK 568
RARG+LI+E+ +SF+VDAFIGN TDWEKVC+GNLVGLPV+V+PTGF NI+D P+ RR+
Sbjct: 532 RARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFKNITDPPTNSCRRR 591
Query: 569 TTVTTGVYAPPNRDHIALALAMAYQEVTNHHKQRPPINDLGPNDKIPNPTNVTYPAR 625
TT+ G+YAPP RDHIALAL MAYQ VT+ H++RPPI+DLGP+D IPNP P R
Sbjct: 592 TTINAGIYAPPERDHIALALGMAYQSVTDAHRKRPPIDDLGPDDSIPNPPRALIPPR 648
>AT3G25660.1 | Symbols: | Amidase family protein |
chr3:9339640-9342044 REVERSE LENGTH=537
Length = 537
Score = 105 bits (261), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 65/231 (28%), Positives = 113/231 (48%), Gaps = 14/231 (6%)
Query: 182 LQTKQITSQELTQIFLKRLKKYNPILEAVITYTEELAHNQAKEADKLLSQGVYLGPLHGI 241
L + + T+ E+ + +L R++ P L+ + +E + + A+E D+ +++G LGPL G+
Sbjct: 53 LLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKD-AQEIDQRIAKGEELGPLAGV 111
Query: 242 PYGLKDIISVPGYKTTWGSKTFKNQVINMEAWVYKRLKSAGAVLVAKLVSGSLAYDDIWF 301
G+KD I G +T S+ ++ +A K++K G ++V G D+
Sbjct: 112 LIGVKDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVV-----GKTNMDEFGM 166
Query: 302 GGRTR--------NPWNIEEFXXXXXXXXXXXXXXXLVPFAIGSETAGSITYPAARCGVT 353
G T NPW++ ++GS+T GS+ PA+ CGV
Sbjct: 167 GSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVV 226
Query: 354 ALRPTFGTVGRTGVMSLSESLDKLGPFCRSATDCAVVLDIVRGRDPNDLSS 404
L+PT+G V R G+M+ + SLD +G F + D ++L + G D D +S
Sbjct: 227 GLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTS 277
>AT4G34880.1 | Symbols: | Amidase family protein |
chr4:16615549-16617424 FORWARD LENGTH=466
Length = 466
Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 66/226 (29%), Positives = 107/226 (47%), Gaps = 40/226 (17%)
Query: 185 KQITSQELTQIFLKRLKKYNPILEAVI-TYTEELAHNQAKEADKLLSQGVYLGPLHGIPY 243
K++TS++L +++L+ + K NPIL AVI T + L + + ++ L L LHG+P
Sbjct: 55 KRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPV 114
Query: 244 GLKDIISVPG-YKTTWGSKTFKNQVINMEAWVYKRLKSAGAVLVAKLVSGSLAYDDIWFG 302
LKD IS TT GS V+ +A V KRL+ +GAV++ K SL+ W
Sbjct: 115 LLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGK---ASLSE---WAH 168
Query: 303 GRTRNPWNIEEFXXXXXXXXXXXXXXXLVPFAIGSETAGSITYPAARCGVTALRPTFGTV 362
R+ + +P + + S V ++P+ G
Sbjct: 169 FRSFS-----------------------IPDGWSAPSQNS---------VVGIKPSVGLT 196
Query: 363 GRTGVMSLSESLDKLGPFCRSATDCAVVLDIVRGRDPNDLSSRHSS 408
R GV+ +S D +GP CR+ +D +LD + G DP D +++ +S
Sbjct: 197 SRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTAS 242
>AT1G08980.1 | Symbols: ATAMI1, AMI1, ATTOC64-I, TOC64-I | amidase 1
| chr1:2884455-2886430 FORWARD LENGTH=425
Length = 425
Score = 67.8 bits (164), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 43/150 (28%), Positives = 67/150 (44%), Gaps = 5/150 (3%)
Query: 238 LHGIPYGLKDIISVPGYKTTWGSKTF--KNQVINMEAWVYKRLKSAGAVLVAKLVSGSLA 295
L G+ + +KDI V G T +G+ + + A V L AGA + + +A
Sbjct: 28 LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87
Query: 296 YD---DIWFGGRTRNPWNIEEFXXXXXXXXXXXXXXXLVPFAIGSETAGSITYPAARCGV 352
Y + G RNP + LV F+IG++T GS+ PA+ CG+
Sbjct: 88 YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGI 147
Query: 353 TALRPTFGTVGRTGVMSLSESLDKLGPFCR 382
RP+ G V G+ +++S D +G F R
Sbjct: 148 FGFRPSHGAVSTVGLTPMAQSFDTVGWFAR 177
>AT5G64440.1 | Symbols: AtFAAH, FAAH | fatty acid amide hydrolase |
chr5:25766229-25770260 FORWARD LENGTH=607
Length = 607
Score = 67.4 bits (163), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 48/187 (25%), Positives = 86/187 (45%), Gaps = 8/187 (4%)
Query: 221 QAKEADKLLSQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKTF-KNQVINMEAWVYKRLK 279
QA+ + + QG + L GI +KD I + T G+ +++ + ++ V +L+
Sbjct: 180 QAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLR 239
Query: 280 SAGAVLVAK-----LVSGSLAYDDIWFGGRTRNPWNIEEFXXXXXXXXXXXXXXXLVPFA 334
S GA+L+ K L G+ + + G TRNP + + + L A
Sbjct: 240 SCGAILLGKANMHELGMGTTGNNSNY--GTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAA 297
Query: 335 IGSETAGSITYPAARCGVTALRPTFGTVGRTGVMSLSESLDKLGPFCRSATDCAVVLDIV 394
+G++ GS+ P+A CG+T L+ T+G TG + +++ +GP S D +V +
Sbjct: 298 LGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAI 357
Query: 395 RGRDPND 401
G D
Sbjct: 358 LGSSSAD 364
>AT5G09420.1 | Symbols: ATTOC64-V, MTOM64, TOC64-V, OM64, AtmtOM64 |
translocon at the outer membrane of chloroplasts 64-V |
chr5:2928316-2931750 FORWARD LENGTH=603
Length = 603
Score = 59.7 bits (143), Expect = 7e-09, Method: Compositional matrix adjust.
Identities = 47/166 (28%), Positives = 72/166 (43%), Gaps = 18/166 (10%)
Query: 238 LHGIPYGLKDIISVPGYKTTWGSKTFK--NQVINMEAWVYKRLKSAGAVLVAKLVSGSLA 295
L G+ + + D V Y T +G +K ++ A V L GA V K +
Sbjct: 71 LSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKTAVVVTTLLKNGATCVGKTI----- 125
Query: 296 YDDIWFG--------GRTRNPWNIEEFXXXXXXXXXXXXXXXLVPFAIGSETAGSITYPA 347
D++ FG G NP + LV F++G +T G + PA
Sbjct: 126 MDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGAELVDFSLGIDTTGGVRVPA 185
Query: 348 ARCGVTALRPTFGTVGRTGVMSLSESLDKLGPFCRSATDCAVVLDI 393
A CG+ RP+ GTV GV+ S+SL+ +G F A+D +V+ +
Sbjct: 186 AFCGILGFRPSQGTVSSVGVLPNSQSLETVGWF---ASDPSVLCQV 228
>AT3G17970.1 | Symbols: atToc64-III, TOC64-III | translocon at the
outer membrane of chloroplasts 64-III |
chr3:6148030-6151794 FORWARD LENGTH=589
Length = 589
Score = 53.9 bits (128), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 38/151 (25%), Positives = 62/151 (41%), Gaps = 11/151 (7%)
Query: 240 GIPYGLKDIISVPGYKTTWGSKTF--KNQVINMEAWVYKRLKSAGAVLVAKLVSGSLAYD 297
G+ + + D+ + GY T +G + ++ + + V L GA V K V A+
Sbjct: 60 GLTFAVSDVFDITGYVTGFGHPDWVRTHEAASSTSPVVSTLVEGGATCVGKTVVDEFAFS 119
Query: 298 DIWFGGRTR------NPWNIEEFXXXXXXXXXXXXXXXLVPFAIGSETAGSITYPAARCG 351
G + NP V FA+G +T G + PA CG
Sbjct: 120 ---ISGENKHYDSPTNPAAPTRIPGGACSGAAVAVATNAVDFALGIDTVGGVRVPAGYCG 176
Query: 352 VTALRPTFGTVGRTGVMSLSESLDKLGPFCR 382
V + ++G + TG++ +S SLD +G F R
Sbjct: 177 VLGFKSSYGAISNTGIIPVSSSLDSVGWFAR 207