Miyakogusa Predicted Gene

Lj4g3v2370450.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2370450.1 Non Characterized Hit- tr|I1KN20|I1KN20_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.13332
PE,93.38,0,EF_HAND_2,EF-HAND 2; coiled-coil,NULL; no
description,EF-hand-like domain; EF-hand,NULL;
EF_HAND_1,E,CUFF.50838.1
         (409 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g119230.1 | serine/threonine-protein phosphatase 2A regula...   723   0.0  
Medtr4g119230.2 | serine/threonine-protein phosphatase 2A regula...   559   e-159
Medtr1g075800.2 | calcium-binding EF-hand protein | HC | chr1:33...    69   6e-12
Medtr1g075800.1 | calcium-binding EF-hand protein | HC | chr1:33...    69   8e-12
Medtr1g080030.1 | calcium-binding EF-hand protein | HC | chr1:35...    59   8e-09
Medtr7g116150.1 | calcium-binding EF-hand protein | HC | chr7:47...    56   5e-08

>Medtr4g119230.1 | serine/threonine-protein phosphatase 2A
           regulatory subunit B | HC | chr4:49372062-49365463 |
           20130731
          Length = 475

 Score =  723 bits (1865), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 349/411 (84%), Positives = 364/411 (88%), Gaps = 2/411 (0%)

Query: 1   MYSDGETQDA--SLRKIPPASSMPWARNLRRFIGSGAGLGSEALMELETKRILFGIFKET 58
           MYSDGE  DA  + RKIPPASSM W RNLRRFIGSGAGLGSEALMELETKRIL  IFK+ 
Sbjct: 1   MYSDGEAHDAPQTQRKIPPASSMLWVRNLRRFIGSGAGLGSEALMELETKRILLDIFKDK 60

Query: 59  QKKSAEASTIPSFYKKKPEEGSISHRVQRLAKYRFLKKQSXXXXXXXXXXXMWVCLRGNC 118
           QKKSAEA TIPSFYKKKPEEGSIS RVQRLAKYRFLKKQS           MWVCLR NC
Sbjct: 61  QKKSAEAGTIPSFYKKKPEEGSISQRVQRLAKYRFLKKQSDLLLNADDLDAMWVCLRENC 120

Query: 119 VIDDASGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRIAILPFYLYV 178
           VIDDA+GAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRIAILPFYLYV
Sbjct: 121 VIDDATGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRIAILPFYLYV 180

Query: 179 MRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAAH 238
           MRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLR M + FV MYCRIAAH
Sbjct: 181 MRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLRTMDAGFVPMYCRIAAH 240

Query: 239 KFFFFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDM 298
           KFFFFCDPHRRGKACIKKVLLSNC              VTDTEQAENWFS TSA+R+CDM
Sbjct: 241 KFFFFCDPHRRGKACIKKVLLSNCLQELMELHQESEEEVTDTEQAENWFSQTSARRVCDM 300

Query: 299 FLALDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRRGKTGGGNSREMDFESFLDFV 358
           F+ALDKD++G+LSK ELREYADGTLTEIFIERV+DEHVRRGK+ GG +REMDFESFLDF 
Sbjct: 301 FIALDKDSSGSLSKHELREYADGTLTEIFIERVFDEHVRRGKSCGGKTREMDFESFLDFN 360

Query: 359 LALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRDVHHKWIEGGNYEL 409
           LALENKDTPEG+TYLFRCLDLQGRGYLTTADIHSLFRDVHHKWI+GGNYEL
Sbjct: 361 LALENKDTPEGLTYLFRCLDLQGRGYLTTADIHSLFRDVHHKWIDGGNYEL 411


>Medtr4g119230.2 | serine/threonine-protein phosphatase 2A
           regulatory subunit B | HC | chr4:49371509-49365514 |
           20130731
          Length = 364

 Score =  559 bits (1440), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 263/300 (87%), Positives = 276/300 (92%)

Query: 110 MWVCLRGNCVIDDASGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRI 169
           MWVCLR NCVIDDA+GAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRI
Sbjct: 1   MWVCLRENCVIDDATGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRI 60

Query: 170 AILPFYLYVMRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFV 229
           AILPFYLYVMRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLR M + FV
Sbjct: 61  AILPFYLYVMRTVSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLRTMDAGFV 120

Query: 230 QMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSL 289
            MYCRIAAHKFFFFCDPHRRGKACIKKVLLSNC              VTDTEQAENWFS 
Sbjct: 121 PMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNCLQELMELHQESEEEVTDTEQAENWFSQ 180

Query: 290 TSAQRICDMFLALDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRRGKTGGGNSREM 349
           TSA+R+CDMF+ALDKD++G+LSK ELREYADGTLTEIFIERV+DEHVRRGK+ GG +REM
Sbjct: 181 TSARRVCDMFIALDKDSSGSLSKHELREYADGTLTEIFIERVFDEHVRRGKSCGGKTREM 240

Query: 350 DFESFLDFVLALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRDVHHKWIEGGNYEL 409
           DFESFLDF LALENKDTPEG+TYLFRCLDLQGRGYLTTADIHSLFRDVHHKWI+GGNYEL
Sbjct: 241 DFESFLDFNLALENKDTPEGLTYLFRCLDLQGRGYLTTADIHSLFRDVHHKWIDGGNYEL 300


>Medtr1g075800.2 | calcium-binding EF-hand protein | HC |
           chr1:33596190-33588147 | 20130731
          Length = 510

 Score = 69.3 bits (168), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 60/260 (23%), Positives = 114/260 (43%), Gaps = 17/260 (6%)

Query: 148 KCRRFFSPSNFMKFEKDEQGRIAILPFYLYVMR--TVSLTQARIDMSELDEDSDGFLQPH 205
           K   FFS + F K + +  G I    F  Y +R   +++       + L +    +L   
Sbjct: 148 KIPSFFSTALFKKIDVNLTGTITRDQFIDYWIRGNMLAMDLPTQIYTILKQPHRTYLVQD 207

Query: 206 EMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNC 262
           + +  +R L+   P L  L+  P  F + Y     H+ F++ +    G+  ++++   N 
Sbjct: 208 DFKPILRELLSSHPGLEFLQSTPE-FQERYAETVIHRIFYYMNRSGNGRLTLRELKRGNL 266

Query: 263 XXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGT 322
                           D  +   +FS      I   F  LD D +  + ++ L +Y + +
Sbjct: 267 IDAMLHVDEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFFIDRENLIKYGNHS 321

Query: 323 LTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQ 380
           LT   ++R++ +  R+   K  G    +M++E F+ F+LA E+K +   + Y FRC+DL 
Sbjct: 322 LTYRIVDRIFSQVPRKFSSKVEG----KMNYEDFVHFILAEEDKSSEPSLEYWFRCIDLD 377

Query: 381 GRGYLTTADIHSLFRDVHHK 400
           G G LT  ++   + +  H+
Sbjct: 378 GNGVLTPNEMLFFYEEQLHR 397


>Medtr1g075800.1 | calcium-binding EF-hand protein | HC |
           chr1:33599089-33588147 | 20130731
          Length = 539

 Score = 68.9 bits (167), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 60/260 (23%), Positives = 114/260 (43%), Gaps = 17/260 (6%)

Query: 148 KCRRFFSPSNFMKFEKDEQGRIAILPFYLYVMR--TVSLTQARIDMSELDEDSDGFLQPH 205
           K   FFS + F K + +  G I    F  Y +R   +++       + L +    +L   
Sbjct: 177 KIPSFFSTALFKKIDVNLTGTITRDQFIDYWIRGNMLAMDLPTQIYTILKQPHRTYLVQD 236

Query: 206 EMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNC 262
           + +  +R L+   P L  L+  P  F + Y     H+ F++ +    G+  ++++   N 
Sbjct: 237 DFKPILRELLSSHPGLEFLQSTPE-FQERYAETVIHRIFYYMNRSGNGRLTLRELKRGNL 295

Query: 263 XXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGT 322
                           D  +   +FS      I   F  LD D +  + ++ L +Y + +
Sbjct: 296 IDAMLHVDEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFFIDRENLIKYGNHS 350

Query: 323 LTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQ 380
           LT   ++R++ +  R+   K  G    +M++E F+ F+LA E+K +   + Y FRC+DL 
Sbjct: 351 LTYRIVDRIFSQVPRKFSSKVEG----KMNYEDFVHFILAEEDKSSEPSLEYWFRCIDLD 406

Query: 381 GRGYLTTADIHSLFRDVHHK 400
           G G LT  ++   + +  H+
Sbjct: 407 GNGVLTPNEMLFFYEEQLHR 426


>Medtr1g080030.1 | calcium-binding EF-hand protein | HC |
           chr1:35561864-35552437 | 20130731
          Length = 537

 Score = 58.9 bits (141), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 58/260 (22%), Positives = 109/260 (41%), Gaps = 18/260 (6%)

Query: 148 KCRRFFSPSNFMKFEKDEQGRIAILPFYLYVMRTVSLTQ--ARIDMSELDEDSDGFLQPH 205
           K   FFS S F K + +  G +    F  Y +    LT   A    + L +    +L   
Sbjct: 176 KLPSFFSTSLFRKID-NGAGVVTREAFIDYWINGNLLTMDLATQIFTILKQPQLNYLTQD 234

Query: 206 EMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNC 262
           + +  +R L+   P L  L+  P  F + Y     ++ +++ +    G+  ++++   N 
Sbjct: 235 DFKPVLRELLASHPGLEFLQSTPE-FQERYAETVVYRIYYYINRTGNGRMTLRELKRGNI 293

Query: 263 XXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGT 322
                           D  +   +FS      I   F  LD D +  + K+ L  Y +  
Sbjct: 294 IDAMQHADEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIRYGNHA 348

Query: 323 LTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQ 380
           LT   ++R++ +  R+   K  G    +M +E F+ F+L+ E+K +   + Y F+C+DL 
Sbjct: 349 LTYRIVDRIFSQVPRKFTSKVEG----KMGYEDFVYFILSEEDKSSEPSLEYWFKCIDLD 404

Query: 381 GRGYLTTADIHSLFRDVHHK 400
             G LT  ++   + +  H+
Sbjct: 405 ANGVLTRNELQFFYEEQLHR 424


>Medtr7g116150.1 | calcium-binding EF-hand protein | HC |
           chr7:47881004-47889401 | 20130731
          Length = 524

 Score = 56.2 bits (134), Expect = 5e-08,   Method: Compositional matrix adjust.
 Identities = 34/124 (27%), Positives = 61/124 (49%), Gaps = 6/124 (4%)

Query: 279 DTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRR 338
           D  +   +FS      I   F  LD D +  + K+ L  Y +  LT   ++R++ +  R+
Sbjct: 292 DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIRYGNHALTYRIVDRIFSQVPRK 351

Query: 339 --GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRD 396
              K  G    +M +E F+ F+L+ E+K +   + Y F+C+DL G G LT  ++   + +
Sbjct: 352 FTSKVEG----KMGYEDFVYFILSEEDKSSDPSLEYWFKCVDLDGNGVLTRNELQFFYEE 407

Query: 397 VHHK 400
             H+
Sbjct: 408 QLHR 411