Miyakogusa Predicted Gene

Lj4g3v2370450.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v2370450.1 Non Chatacterized Hit- tr|I1KN20|I1KN20_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.13332
PE,93.38,0,EF_HAND_2,EF-HAND 2; coiled-coil,NULL; no
description,EF-hand-like domain; EF-hand,NULL;
EF_HAND_1,E,CUFF.50838.1
         (409 letters)

Database: TAIR10_pep 
           35,386 sequences; 14,482,855 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G18580.1 | Symbols: FASS, TON2, EMB40, FS1, GDO, FASS 2 | ton...   704   0.0  
AT5G28900.1 | Symbols:  | Calcium-binding EF-hand family protein...    65   6e-11
AT5G28850.2 | Symbols:  | Calcium-binding EF-hand family protein...    65   7e-11
AT1G54450.1 | Symbols:  | Calcium-binding EF-hand family protein...    64   1e-10
AT5G44090.1 | Symbols:  | Calcium-binding EF-hand family protein...    64   2e-10
AT1G03960.1 | Symbols:  | Calcium-binding EF hand family protein...    64   2e-10
AT1G03960.2 | Symbols:  | Calcium-binding EF hand family protein...    64   3e-10
AT5G28850.1 | Symbols:  | Calcium-binding EF-hand family protein...    60   4e-09

>AT5G18580.1 | Symbols: FASS, TON2, EMB40, FS1, GDO, FASS 2 |
           tonneau 2 (TON2) | chr5:6175154-6178214 FORWARD
           LENGTH=480
          Length = 480

 Score =  704 bits (1817), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 340/408 (83%), Positives = 356/408 (87%), Gaps = 1/408 (0%)

Query: 3   SDGETQDASL-RKIPPASSMPWARNLRRFIGSGAGLGSEALMELETKRILFGIFKETQKK 61
           SDGE+ D S  RKIPPASSM W RNLRR+IGSGAGLGSEALMELETKRIL  IFKE Q+K
Sbjct: 6   SDGESHDTSTQRKIPPASSMLWVRNLRRYIGSGAGLGSEALMELETKRILLEIFKEKQQK 65

Query: 62  SAEASTIPSFYKKKPEEGSISHRVQRLAKYRFLKKQSXXXXXXXXXXXMWVCLRGNCVID 121
           S EA TIPSFYKKKPEEGSIS RVQ+LAKYRFLKKQS           MWVCLR NCVID
Sbjct: 66  SQEAGTIPSFYKKKPEEGSISQRVQKLAKYRFLKKQSDLLLNADDLAAMWVCLRENCVID 125

Query: 122 DASGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEQGRIAILPFYLYVMRT 181
           DA+GAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDE GRIAILPFYLYVMRT
Sbjct: 126 DATGAEKMNYEDFCHIASVCTEQIGPKCRRFFSPSNFMKFEKDEAGRIAILPFYLYVMRT 185

Query: 182 VSLTQARIDMSELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAAHKFF 241
           VSLTQARIDMSELDEDSDGFL   EME+YI GLIPNLAQLRDMP AF QMYCRIA+ KFF
Sbjct: 186 VSLTQARIDMSELDEDSDGFLHSDEMESYIGGLIPNLAQLRDMPPAFNQMYCRIASQKFF 245

Query: 242 FFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLA 301
           FFCDPHRRG+ACIKK+LLSNC              VTDTEQAENWFSLTSAQRICDMFLA
Sbjct: 246 FFCDPHRRGRACIKKILLSNCLQELMELHQESEEEVTDTEQAENWFSLTSAQRICDMFLA 305

Query: 302 LDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRRGKTGGGNSREMDFESFLDFVLAL 361
           LDKD +G+L KQEL+EYADGTLTEIFIERV+DEHVRRGK   GNSREMDF+SFLDFVLAL
Sbjct: 306 LDKDMSGSLCKQELKEYADGTLTEIFIERVFDEHVRRGKIVAGNSREMDFDSFLDFVLAL 365

Query: 362 ENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRDVHHKWIEGGNYEL 409
           ENKDTPEG+TYLFRCLDLQGRG+LTTADIHSLFRDVH KWIEGGNYEL
Sbjct: 366 ENKDTPEGLTYLFRCLDLQGRGFLTTADIHSLFRDVHQKWIEGGNYEL 413


>AT5G28900.1 | Symbols:  | Calcium-binding EF-hand family protein |
           chr5:10925852-10929892 FORWARD LENGTH=536
          Length = 536

 Score = 65.5 bits (158), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 58/261 (22%), Positives = 110/261 (42%), Gaps = 19/261 (7%)

Query: 148 KCRRFFSPSNFMKFEKDEQG---RIAILPFYLYVMRTVSLTQARIDMSELDEDSDGFLQP 204
           K   FFS S F K + +  G   R A + F++     +  T  +I    L +    F+  
Sbjct: 173 KLPSFFSTSLFRKIDLNNTGFVTRDAFIDFWVNGNMLIMDTTTQI-FKILKQKDQSFIVK 231

Query: 205 HEMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSN 261
            + +  ++ L+   P L  L+  P  F + Y     ++ F++ +    G+   +++   N
Sbjct: 232 DDFKPLLKELLATHPGLEFLQSTPE-FQERYAETVTYRIFYYINRSGNGRITFRELKRGN 290

Query: 262 CXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADG 321
                            D  +   +FS      I   F  LD D +  + K+ L  Y + 
Sbjct: 291 LIDAMLHADEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLMRYGNH 345

Query: 322 TLTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDL 379
            LT   ++R++ +  R+   K  G    +M +E F+ F+LA E+K +   + Y F+C+DL
Sbjct: 346 ALTYRIVDRIFSQVARKFTNKVEG----KMGYEDFVYFILAEEDKSSVPSLEYWFKCIDL 401

Query: 380 QGRGYLTTADIHSLFRDVHHK 400
              G +T  ++   + +  H+
Sbjct: 402 DANGIITRNEMQFFYEEQLHR 422


>AT5G28850.2 | Symbols:  | Calcium-binding EF-hand family protein |
           chr5:10877360-10881278 REVERSE LENGTH=536
          Length = 536

 Score = 65.5 bits (158), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 58/261 (22%), Positives = 110/261 (42%), Gaps = 19/261 (7%)

Query: 148 KCRRFFSPSNFMKFEKDEQG---RIAILPFYLYVMRTVSLTQARIDMSELDEDSDGFLQP 204
           K   FFS S F K + +  G   R A + F++     +  T  +I    L +    F+  
Sbjct: 173 KLPSFFSTSLFRKIDLNNTGFVTRDAFIDFWVNGNMLIMDTTTQI-FKILKQKDQSFIVK 231

Query: 205 HEMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSN 261
            + +  ++ L+   P L  L+  P  F + Y     ++ F++ +    G+   +++   N
Sbjct: 232 DDFKPLLKELLATHPGLEFLQSTPE-FQERYAETVTYRIFYYINRSGNGRITFRELKRGN 290

Query: 262 CXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADG 321
                            D  +   +FS      I   F  LD D +  + K+ L  Y + 
Sbjct: 291 LIDAMLHADEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLMRYGNH 345

Query: 322 TLTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDL 379
            LT   ++R++ +  R+   K  G    +M +E F+ F+LA E+K +   + Y F+C+DL
Sbjct: 346 ALTYRIVDRIFSQVARKFTSKVEG----KMGYEDFVYFILAEEDKSSVPSLEYWFKCIDL 401

Query: 380 QGRGYLTTADIHSLFRDVHHK 400
              G +T  ++   + +  H+
Sbjct: 402 DANGIITRNEMQFFYEEQLHR 422


>AT1G54450.1 | Symbols:  | Calcium-binding EF-hand family protein |
           chr1:20336570-20339535 REVERSE LENGTH=535
          Length = 535

 Score = 64.3 bits (155), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 58/260 (22%), Positives = 110/260 (42%), Gaps = 17/260 (6%)

Query: 148 KCRRFFSPSNFMKFEKDEQGRIAILPFYLYVMRTVSLTQARID--MSELDEDSDGFLQPH 205
           K   FFS S F K + +  G +    F  Y ++   LT+       + L +    +L   
Sbjct: 173 KVPSFFSTSIFKKVDTNNTGFVKREDFIDYWVKGNMLTKEITSQVFTILKQPDHNYLVQD 232

Query: 206 EMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNC 262
           + +  ++ L+   P L  L+  P  F   Y     ++ +++ +    G   ++++   N 
Sbjct: 233 DFKPVLQELLATHPGLEFLQGTPE-FQDRYAETVIYRIYYYINRSGNGHLTLRELKRGNL 291

Query: 263 XXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGT 322
                           D  +   +FS      I   F  LD D +  + K+ L  Y++  
Sbjct: 292 VDAMQHADEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIRYSNHA 346

Query: 323 LTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQ 380
           LT   ++R++ +  R+   KT G    +M +E F+ F+LA E+K +   + Y F+C+DL 
Sbjct: 347 LTYRIVDRIFSQVPRKFTSKTEG----KMGYEDFVYFILAEEDKSSEPSLEYWFKCIDLD 402

Query: 381 GRGYLTTADIHSLFRDVHHK 400
             G LT  ++   + +  H+
Sbjct: 403 ANGVLTRNELQFFYEEQLHR 422


>AT5G44090.1 | Symbols:  | Calcium-binding EF-hand family protein |
           chr5:17743264-17747909 REVERSE LENGTH=538
          Length = 538

 Score = 64.3 bits (155), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 47/187 (25%), Positives = 81/187 (43%), Gaps = 12/187 (6%)

Query: 216 PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXX 275
           P L  LR+ P  F + Y     ++ F++ +  R G  CI    L                
Sbjct: 249 PGLEFLRNTPE-FQERYAETVIYRIFYYIN--RSGTGCIT---LRELKRGNLITAMQQVD 302

Query: 276 XVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGTLTEIFIERVYDEH 335
              D  +   +FS      I   F  LD D +  + K+ L +Y +  LT   ++R++ + 
Sbjct: 303 EEDDINKVIRYFSYEHFYVIYCRFWELDGDHDFLIDKENLIKYGNHALTYRIVDRIFSQV 362

Query: 336 VRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSL 393
            R+   K  G    +M +E F  F+LA E+K +   + Y F+C+DL G G +T  ++   
Sbjct: 363 PRKFTSKVEG----KMSYEDFAYFILAEEDKSSEPSLEYWFKCIDLDGDGVITPNEMQFF 418

Query: 394 FRDVHHK 400
           + +  H+
Sbjct: 419 YEEQLHR 425


>AT1G03960.1 | Symbols:  | Calcium-binding EF hand family protein |
           chr1:1014085-1017472 FORWARD LENGTH=529
          Length = 529

 Score = 64.3 bits (155), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 67/281 (23%), Positives = 121/281 (43%), Gaps = 22/281 (7%)

Query: 130 NYEDFCHIASV--CTEQIGPKCRRFFSPSNFMKFEK---DEQGRIAILPFYLYVMRTVSL 184
           NY D  H+      T+Q+  K   F SP+ F K +    D   R A + +++        
Sbjct: 148 NYIDGLHVDEFKSITKQVC-KLPSFLSPALFRKIDPNCTDIVTRDAFIKYWIDGNMLTMD 206

Query: 185 TQARIDMSELDEDSDGFLQPHEMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFF 241
           T ++I  + L +    +L+  + +  +  L+   P L  LR + S F + Y     ++ F
Sbjct: 207 TASQI-YNILRQQGCSYLRQADFKPVLDELLATHPGLEFLRTI-SEFQERYAETVIYRIF 264

Query: 242 FFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLA 301
           ++   +R G  C+    L                   D  +   +FS      I   F  
Sbjct: 265 YYI--NRSGTGCLT---LRELRRGNLIAAMQQLDEEDDINKIIRYFSYEHFYVIYCKFWE 319

Query: 302 LDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVL 359
           LD D +  + K  L +Y +  LT   ++R++ +  R+   K  G    +M +E F+ F+L
Sbjct: 320 LDGDHDCFIDKDNLIKYGNNALTYRIVDRIFSQIPRKFTSKVEG----KMSYEDFVYFIL 375

Query: 360 ALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRDVHHK 400
           A E+K +   + Y F+C+DL G G +T+ ++   F +  H+
Sbjct: 376 AEEDKSSEPSLEYWFKCVDLDGNGVITSNEMQFFFEEQLHR 416


>AT1G03960.2 | Symbols:  | Calcium-binding EF hand family protein |
           chr1:1014824-1017472 FORWARD LENGTH=389
          Length = 389

 Score = 63.5 bits (153), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 67/281 (23%), Positives = 121/281 (43%), Gaps = 22/281 (7%)

Query: 130 NYEDFCHIASV--CTEQIGPKCRRFFSPSNFMKFE---KDEQGRIAILPFYLYVMRTVSL 184
           NY D  H+      T+Q+  K   F SP+ F K +    D   R A + +++        
Sbjct: 8   NYIDGLHVDEFKSITKQVC-KLPSFLSPALFRKIDPNCTDIVTRDAFIKYWIDGNMLTMD 66

Query: 185 TQARIDMSELDEDSDGFLQPHEMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAAHKFF 241
           T ++I  + L +    +L+  + +  +  L+   P L  LR + S F + Y     ++ F
Sbjct: 67  TASQI-YNILRQQGCSYLRQADFKPVLDELLATHPGLEFLRTI-SEFQERYAETVIYRIF 124

Query: 242 FFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLA 301
           ++   +R G  C+    L                   D  +   +FS      I   F  
Sbjct: 125 YYI--NRSGTGCLT---LRELRRGNLIAAMQQLDEEDDINKIIRYFSYEHFYVIYCKFWE 179

Query: 302 LDKDANGTLSKQELREYADGTLTEIFIERVYDEHVRR--GKTGGGNSREMDFESFLDFVL 359
           LD D +  + K  L +Y +  LT   ++R++ +  R+   K  G    +M +E F+ F+L
Sbjct: 180 LDGDHDCFIDKDNLIKYGNNALTYRIVDRIFSQIPRKFTSKVEG----KMSYEDFVYFIL 235

Query: 360 ALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSLFRDVHHK 400
           A E+K +   + Y F+C+DL G G +T+ ++   F +  H+
Sbjct: 236 AEEDKSSEPSLEYWFKCVDLDGNGVITSNEMQFFFEEQLHR 276


>AT5G28850.1 | Symbols:  | Calcium-binding EF-hand family protein |
           chr5:10877360-10879416 REVERSE LENGTH=324
          Length = 324

 Score = 59.7 bits (143), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 42/187 (22%), Positives = 80/187 (42%), Gaps = 12/187 (6%)

Query: 216 PNLAQLRDMPSAFVQMYCRIAAHKFFFFCDPHRRGKACIKKVLLSNCXXXXXXXXXXXXX 275
           P L  L+  P  F + Y     ++ F++ +    G+   +++   N              
Sbjct: 34  PGLEFLQSTPE-FQERYAETVTYRIFYYINRSGNGRITFRELKRGNLIDAMLHADEE--- 89

Query: 276 XVTDTEQAENWFSLTSAQRICDMFLALDKDANGTLSKQELREYADGTLTEIFIERVYDEH 335
              D  +   +FS      I   F  LD D +  + K+ L  Y +  LT   ++R++ + 
Sbjct: 90  --EDINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLMRYGNHALTYRIVDRIFSQV 147

Query: 336 VRR--GKTGGGNSREMDFESFLDFVLALENKDTPEGMTYLFRCLDLQGRGYLTTADIHSL 393
            R+   K  G    +M +E F+ F+LA E+K +   + Y F+C+DL   G +T  ++   
Sbjct: 148 ARKFTSKVEG----KMGYEDFVYFILAEEDKSSVPSLEYWFKCIDLDANGIITRNEMQFF 203

Query: 394 FRDVHHK 400
           + +  H+
Sbjct: 204 YEEQLHR 210